Ghent University Academic Bibliography

Advanced

The impact of space flight on survival and interaction of Cupriavidus metallidurans CH34 with basalt, a volcanic moon analog rock

Bo Byloos, Ilse Coninx, Olivier Van Hoey, Charles Cockell, Natasha Nicholson, Vyacheslav Ilyin, Rob Van Houdt, Nico Boon UGent and Natalie Leys (2017) FRONTIERS IN MICROBIOLOGY. 8.
abstract
Microbe-mineral interactions have become of interest for space exploration as microorganisms could be used to biomine from extra-terrestrial material and extract elements useful as micronutrients in life support systems. This research aimed to identify the impact of space flight on the long-term survival of Cupriavidus metallidurans CH34 in mineral water and the interaction with basalt, a lunar-type rock in preparation for the ESA spaceflight experiment, BIOROCK. Therefore, C. metallidurans CH34 cells were suspended in mineral water supplemented with or without crushed basalt and send for 3 months on board the Russian FOTON-M4 capsule. Long-term storage had a significant impact on cell physiology and energy status (by flow cytometry analysis, plate count and intracellular ATP measurements) as 60% of cells stored on ground lost their cell membrane potential, only 17% were still active, average ATP levels per cell were significantly lower and cultivability dropped to 1%. The cells stored in the presence of basalt and exposed to space flight conditions during storage however showed less dramatic changes in physiology, with only 16% of the cells lost their cell membrane potential and 24% were still active, leading to a higher cultivability (50%) and indicating a general positive effect of basalt and space flight on survival. Microbe-mineral interactions and biofilm formation was altered by spaceflight as less biofilm was formed on the basalt during flight conditions. Leaching from basalt also changed (measured with ICP-OES), showing that cells release more copper from basalt and the presence of cells also impacted iron and magnesium concentration irrespective of the presence of basalt. The flight conditions thus could counteract some of the detrimental effects observed after the 3 month storage conditions.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
microbe-mineral interactions, space flight, Cupriavidus metallidurans CH34, basalt, FOTON, FLOW-CYTOMETRY, RALSTONIA-EUTROPHA, SIMULATED MICROGRAVITY, ALCALIGENES-EUTROPHUS, MICROBIAL VIABILITY, BIOFILM FORMATION, GENE-EXPRESSION, BACTERIA, ENVIRONMENTS, STATION
journal title
FRONTIERS IN MICROBIOLOGY
Front. Microbiol.
volume
8
article number
671
pages
14 pages
Web of Science type
Article
Web of Science id
000400237200001
ISSN
1664-302X
DOI
10.3389/fmicb.2017.00671
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8522539
handle
http://hdl.handle.net/1854/LU-8522539
date created
2017-06-06 09:21:38
date last changed
2017-06-20 13:43:11
@article{8522539,
  abstract     = {Microbe-mineral interactions have become of interest for space exploration as microorganisms could be used to biomine from extra-terrestrial material and extract elements useful as micronutrients in life support systems. This research aimed to identify the impact of space flight on the long-term survival of Cupriavidus metallidurans CH34 in mineral water and the interaction with basalt, a lunar-type rock in preparation for the ESA spaceflight experiment, BIOROCK. Therefore, C. metallidurans CH34 cells were suspended in mineral water supplemented with or without crushed basalt and send for 3 months on board the Russian FOTON-M4 capsule. Long-term storage had a significant impact on cell physiology and energy status (by flow cytometry analysis, plate count and intracellular ATP measurements) as 60\% of cells stored on ground lost their cell membrane potential, only 17\% were still active, average ATP levels per cell were significantly lower and cultivability dropped to 1\%. The cells stored in the presence of basalt and exposed to space flight conditions during storage however showed less dramatic changes in physiology, with only 16\% of the cells lost their cell membrane potential and 24\% were still active, leading to a higher cultivability (50\%) and indicating a general positive effect of basalt and space flight on survival. Microbe-mineral interactions and biofilm formation was altered by spaceflight as less biofilm was formed on the basalt during flight conditions. Leaching from basalt also changed (measured with ICP-OES), showing that cells release more copper from basalt and the presence of cells also impacted iron and magnesium concentration irrespective of the presence of basalt. The flight conditions thus could counteract some of the detrimental effects observed after the 3 month storage conditions.},
  articleno    = {671},
  author       = {Byloos, Bo and Coninx, Ilse and Van Hoey, Olivier and Cockell, Charles and Nicholson, Natasha and Ilyin, Vyacheslav and Van Houdt, Rob and Boon, Nico and Leys, Natalie},
  issn         = {1664-302X},
  journal      = {FRONTIERS IN MICROBIOLOGY},
  keyword      = {microbe-mineral interactions,space flight,Cupriavidus metallidurans CH34,basalt,FOTON,FLOW-CYTOMETRY,RALSTONIA-EUTROPHA,SIMULATED MICROGRAVITY,ALCALIGENES-EUTROPHUS,MICROBIAL VIABILITY,BIOFILM FORMATION,GENE-EXPRESSION,BACTERIA,ENVIRONMENTS,STATION},
  language     = {eng},
  pages        = {14},
  title        = {The impact of space flight on survival and interaction of Cupriavidus metallidurans CH34 with basalt, a volcanic moon analog rock},
  url          = {http://dx.doi.org/10.3389/fmicb.2017.00671},
  volume       = {8},
  year         = {2017},
}

Chicago
Byloos, Bo, Ilse Coninx, Olivier Van Hoey, Charles Cockell, Natasha Nicholson, Vyacheslav Ilyin, Rob Van Houdt, Nico Boon, and Natalie Leys. 2017. “The Impact of Space Flight on Survival and Interaction of Cupriavidus Metallidurans CH34 with Basalt, a Volcanic Moon Analog Rock.” Frontiers in Microbiology 8.
APA
Byloos, B., Coninx, I., Van Hoey, O., Cockell, C., Nicholson, N., Ilyin, V., Van Houdt, R., et al. (2017). The impact of space flight on survival and interaction of Cupriavidus metallidurans CH34 with basalt, a volcanic moon analog rock. FRONTIERS IN MICROBIOLOGY, 8.
Vancouver
1.
Byloos B, Coninx I, Van Hoey O, Cockell C, Nicholson N, Ilyin V, et al. The impact of space flight on survival and interaction of Cupriavidus metallidurans CH34 with basalt, a volcanic moon analog rock. FRONTIERS IN MICROBIOLOGY. 2017;8.
MLA
Byloos, Bo, Ilse Coninx, Olivier Van Hoey, et al. “The Impact of Space Flight on Survival and Interaction of Cupriavidus Metallidurans CH34 with Basalt, a Volcanic Moon Analog Rock.” FRONTIERS IN MICROBIOLOGY 8 (2017): n. pag. Print.