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Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders

(2017) PLOS ONE. 12(4).
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Abstract
Brown rats (Rattus norvegicus) have been identified as potential carriers of Yersinia enterocolitica and Y. pseudotuberculosis, the etiological agents of yersiniosis, the third most reported bacterial zoonosis in Europe. Enteropathogenic Yersinia spp. are most often isolated from rats during yersiniosis cases in animals and humans, and from rats inhabiting farms and slaughterhouses. Information is however lacking regarding the extent to which rats act as carriers of these Yersinia spp.. In 2013, 1088 brown rats across Flanders, Belgium, were tested for the presence of Yersinia species by isolation method. Identification was performed using MALDI-TOF MS, PCR on chromosomal-and plasmid-borne virulence genes, biotyping and serotyping. Yersinia spp. were isolated from 38.4% of the rats. Of these, 53.4% were designated Y. enterocolitica, 0.7% Y. pseudotuberculosis and 49.0% other Yersinia species. Two Y. enterocolitica possessing the virF-, ail- and ystA-gene were isolated. Additionally, the ystB-gene was identified in 94.1% of the other Y. enterocolitica isolates, suggestive for biotype 1A. Three of these latter isolates simultaneously possessed the ail-virulence gene. Significantly more Y. enterocolitica were isolated during winter and spring compared to summer. Based on our findings we can conclude that brown rats are frequent carriers for various Yersinia spp., including Y. pseudotuberculosis and (human pathogenic) Y. enterocolitica which are more often isolated during winter and spring.
Keywords
ENTEROCOLITICA BIOTYPE 1A, TOF MASS-SPECTROMETRY, YST GENES, PSEUDOTUBERCULOSIS, IDENTIFICATION, WILDLIFE, STRAINS, FINLAND, MAMMALS

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Citation

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Chicago
Rouffaer, Lieze, Kristof Baert, Anne-Marie Van den Abeele, Ivo Cox, Gerty Vanantwerpen, Lieven De Zutter, Diederik Strubbe, et al. 2017. “Low Prevalence of Human Enteropathogenic Yersinia Spp. in Brown Rats (Rattus Norvegicus) in Flanders.” Plos One 12 (4).
APA
Rouffaer, L., Baert, K., Van den Abeele, A.-M., Cox, I., Vanantwerpen, G., De Zutter, L., Strubbe, D., et al. (2017). Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders. PLOS ONE, 12(4).
Vancouver
1.
Rouffaer L, Baert K, Van den Abeele A-M, Cox I, Vanantwerpen G, De Zutter L, et al. Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders. PLOS ONE. 2017;12(4).
MLA
Rouffaer, Lieze, Kristof Baert, Anne-Marie Van den Abeele, et al. “Low Prevalence of Human Enteropathogenic Yersinia Spp. in Brown Rats (Rattus Norvegicus) in Flanders.” PLOS ONE 12.4 (2017): n. pag. Print.
@article{8522009,
  abstract     = {Brown rats (Rattus norvegicus) have been identified as potential carriers of Yersinia enterocolitica and Y. pseudotuberculosis, the etiological agents of yersiniosis, the third most reported bacterial zoonosis in Europe. Enteropathogenic Yersinia spp. are most often isolated from rats during yersiniosis cases in animals and humans, and from rats inhabiting farms and slaughterhouses. Information is however lacking regarding the extent to which rats act as carriers of these Yersinia spp.. In 2013, 1088 brown rats across Flanders, Belgium, were tested for the presence of Yersinia species by isolation method. Identification was performed using MALDI-TOF MS, PCR on chromosomal-and plasmid-borne virulence genes, biotyping and serotyping. Yersinia spp. were isolated from 38.4\% of the rats. Of these, 53.4\% were designated Y. enterocolitica, 0.7\% Y. pseudotuberculosis and 49.0\% other Yersinia species. Two Y. enterocolitica possessing the virF-, ail- and ystA-gene were isolated. Additionally, the ystB-gene was identified in 94.1\% of the other Y. enterocolitica isolates, suggestive for biotype 1A. Three of these latter isolates simultaneously possessed the ail-virulence gene. Significantly more Y. enterocolitica were isolated during winter and spring compared to summer. Based on our findings we can conclude that brown rats are frequent carriers for various Yersinia spp., including Y. pseudotuberculosis and (human pathogenic) Y. enterocolitica which are more often isolated during winter and spring.},
  articleno    = { e0175648},
  author       = {Rouffaer, Lieze and Baert, Kristof and Van den Abeele, Anne-Marie and Cox, Ivo and Vanantwerpen, Gerty and De Zutter, Lieven and Strubbe, Diederik and Vranckx, Katleen and Lens, Luc and Haesebrouck, Freddy and Delm{\'e}e, Michel and Pasmans, Frank and Martel, An},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  language     = {eng},
  number       = {4},
  pages        = {8},
  title        = {Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders},
  url          = {http://dx.doi.org/10.1371/journal.pone.0175648},
  volume       = {12},
  year         = {2017},
}

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