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Inhibition of Connexin43 hemichannels impairs spatial short-term memory without affecting spatial working memory

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Abstract
Astrocytes are active players in higher brain function as they can release gliotransmitters, which are essential for synaptic plasticity. Various mechanisms have been proposed for gliotransmission, including vesicular mechanisms as well as non-vesicular ones, for example by passive diffusion via connexin hemichannels (HCs). We here investigated whether interfering with connexin43 (Cx43) HCs influenced hippocampal spatial memory. We made use of the peptide Gap19 that blocks HCs but not gap junction channels and is specific for Cx43. To this end, we microinfused transactivator of transcription linked Gap19 (TAT-Gap19) into the brain ventricle of male NMRI mice and assessed spatial memory in a Y maze. We found that the in vivo blockade of Cx43 HCs did not affect the locomotor activity or spatial working memory in a spontaneous alternation Y maze task. Cx43 blockade did however significantly impair the spatial short-term memory in a delayed spontaneous alternation Y maze task. These results indicate that Cx43 HCs play a role in spatial short-term memory.
Keywords
SCOPOLAMINE-INDUCED AMNESIA, GAP-JUNCTION CHANNELS, PREFRONTAL CORTEX, ASTROCYTES, MICE, HIPPOCAMPUS, RELEASE, RATS, GLIOTRANSMITTERS, RECOGNITION, connexin43, Y maze, TAT-Gap19, working memory, short-term memory

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Chicago
Walrave, Laura, Mathieu Vinken, Giulia Albertini, Dimitri De Bundel, Luc Leybaert, and Ilse J. Smolders. 2016. “Inhibition of Connexin43 Hemichannels Impairs Spatial Short-term Memory Without Affecting Spatial Working Memory.” Frontiers in Cellular Neuroscience 10.
APA
Walrave, L., Vinken, M., Albertini, G., De Bundel, D., Leybaert, L., & Smolders, I. J. (2016). Inhibition of Connexin43 hemichannels impairs spatial short-term memory without affecting spatial working memory. FRONTIERS IN CELLULAR NEUROSCIENCE, 10.
Vancouver
1.
Walrave L, Vinken M, Albertini G, De Bundel D, Leybaert L, Smolders IJ. Inhibition of Connexin43 hemichannels impairs spatial short-term memory without affecting spatial working memory. FRONTIERS IN CELLULAR NEUROSCIENCE. 2016;10.
MLA
Walrave, Laura, Mathieu Vinken, Giulia Albertini, et al. “Inhibition of Connexin43 Hemichannels Impairs Spatial Short-term Memory Without Affecting Spatial Working Memory.” FRONTIERS IN CELLULAR NEUROSCIENCE 10 (2016): n. pag. Print.
@article{8521850,
  abstract     = {Astrocytes are active players in higher brain function as they can release gliotransmitters, which are essential for synaptic plasticity. Various mechanisms have been proposed for gliotransmission, including vesicular mechanisms as well as non-vesicular ones, for example by passive diffusion via connexin hemichannels (HCs). We here investigated whether interfering with connexin43 (Cx43) HCs influenced hippocampal spatial memory. We made use of the peptide Gap19 that blocks HCs but not gap junction channels and is specific for Cx43. To this end, we microinfused transactivator of transcription linked Gap19 (TAT-Gap19) into the brain ventricle of male NMRI mice and assessed spatial memory in a Y maze. We found that the in vivo blockade of Cx43 HCs did not affect the locomotor activity or spatial working memory in a spontaneous alternation Y maze task. Cx43 blockade did however significantly impair the spatial short-term memory in a delayed spontaneous alternation Y maze task. These results indicate that Cx43 HCs play a role in spatial short-term memory.},
  articleno    = {288},
  author       = {Walrave, Laura and Vinken, Mathieu and Albertini, Giulia and De Bundel, Dimitri and Leybaert, Luc and Smolders, Ilse J.},
  issn         = {1662-5102},
  journal      = {FRONTIERS IN CELLULAR NEUROSCIENCE},
  keyword      = {SCOPOLAMINE-INDUCED AMNESIA,GAP-JUNCTION CHANNELS,PREFRONTAL CORTEX,ASTROCYTES,MICE,HIPPOCAMPUS,RELEASE,RATS,GLIOTRANSMITTERS,RECOGNITION,connexin43,Y maze,TAT-Gap19,working memory,short-term memory},
  language     = {eng},
  pages        = {10},
  title        = {Inhibition of Connexin43 hemichannels impairs spatial short-term memory without affecting spatial working memory},
  url          = {http://dx.doi.org/10.3389/fncel.2016.00288},
  volume       = {10},
  year         = {2016},
}

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