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Volatile indicators of contamination in tissue cultures

(2017) Acta Horticulturae. 1155. p.239-244
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Abstract
Microbial contamination is the most important reason for losses in both scientific and commercial micropropagation systems. Latent and epiphytic bacteria can hitchhike imperceptibly during many subcultures. They reduce growth, multiplication and rooting or alter the response to in vitro plant growth regulators. In vitro plants, culture vessel, medium and possible endogenous bacteria release a set of volatile aromatic molecules (biogenic volatile organic compounds, BVOC). Because of its high throughput rate and extreme sensitivity, Selected Ion Flow Tube Mass Spectrometry (SIFT-MS) was chosen to distinguish between presumed clean and artificially Escherichia coli contaminated cultures of Ficus benjamina and Spathiphyllum wallisii. Contaminated cultures of F. benjamina and S. wallisii yielded a net BVOC fingerprint that was both different from each other and from the BVOC spectrum of E. coli. This suggests that they release volatiles that result from the specific interaction with E. coli. The availability of a true 100% bacteria free in vitro plant will be necessary to validate this technology for commercial use.
Keywords
Ficus benjamina, latent bacteria, micropropagation, SIFT-MS, Spathiphyllum wallisii, TUBE MASS-SPECTROMETRY, BACTERIAL VOLATILES, ORGANIC-COMPOUNDS, ESCHERICHIA-COLI, SIFT-MS, PLANT

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Citation

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Chicago
Werbrouck, Stefaan, Pieter Verholle, and Herman Van Langenhove. 2017. “Volatile Indicators of Contamination in Tissue Cultures.” In Acta Horticulturae, ed. M Beruto and EA Ozudogru, 1155:239–244. Leuven, Belgium: International Society for Horticultural Science (ISHS).
APA
Werbrouck, Stefaan, Verholle, P., & Van Langenhove, H. (2017). Volatile indicators of contamination in tissue cultures. In M Beruto & E. Ozudogru (Eds.), Acta Horticulturae (Vol. 1155, pp. 239–244). Presented at the 6th International symposium on Production and Establishment of Micropropagated Plants, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Werbrouck S, Verholle P, Van Langenhove H. Volatile indicators of contamination in tissue cultures. In: Beruto M, Ozudogru E, editors. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2017. p. 239–44.
MLA
Werbrouck, Stefaan, Pieter Verholle, and Herman Van Langenhove. “Volatile Indicators of Contamination in Tissue Cultures.” Acta Horticulturae. Ed. M Beruto & EA Ozudogru. Vol. 1155. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2017. 239–244. Print.
@inproceedings{8521165,
  abstract     = {Microbial contamination is the most important reason for losses in both scientific and commercial micropropagation systems. Latent and epiphytic bacteria can hitchhike imperceptibly during many subcultures. They reduce growth, multiplication and rooting or alter the response to in vitro plant growth regulators. In vitro plants, culture vessel, medium and possible endogenous bacteria release a set of volatile aromatic molecules (biogenic volatile organic compounds, BVOC). Because of its high throughput rate and extreme sensitivity, Selected Ion Flow Tube Mass Spectrometry (SIFT-MS) was chosen to distinguish between presumed clean and artificially Escherichia coli contaminated cultures of Ficus benjamina and Spathiphyllum wallisii. Contaminated cultures of F. benjamina and S. wallisii yielded a net BVOC fingerprint that was both different from each other and from the BVOC spectrum of E. coli. This suggests that they release volatiles that result from the specific interaction with E. coli. The availability of a true 100\% bacteria free in vitro plant will be necessary to validate this technology for commercial use.},
  author       = {Werbrouck, Stefaan and Verholle, Pieter and Van Langenhove, Herman},
  booktitle    = {Acta Horticulturae},
  editor       = {Beruto, M and Ozudogru, EA},
  isbn         = {9789462611511},
  issn         = {0567-7572},
  keyword      = {Ficus benjamina,latent bacteria,micropropagation,SIFT-MS,Spathiphyllum wallisii,TUBE MASS-SPECTROMETRY,BACTERIAL VOLATILES,ORGANIC-COMPOUNDS,ESCHERICHIA-COLI,SIFT-MS,PLANT},
  language     = {eng},
  location     = {Sanremo, Italy},
  pages        = {239--244},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {Volatile indicators of contamination in tissue cultures},
  url          = {http://dx.doi.org/10.17660/actahortic.2017.1155.34},
  volume       = {1155},
  year         = {2017},
}

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