Advanced search
1 file | 213.71 KB

Infrared analysis of lipoproteins in the detection of alcohol biomarkers

Sander De Bruyne (UGent) , Tinne Monteyne (UGent) , Marijn Speeckaert (UGent) and Joris Delanghe (UGent)
Author
Organization
Abstract
Background: Alcoholism is a major public health problem. Alcohol causes modifications in the composition and concentration of lipoproteins and influences the enzymes and transfer proteins that transform lipoproteins in plasma. Alcohol is associated with the presence of alcohol biomarkers (fatty acid ethyl esters [FAEEs] and phosphatidylethanol [PEth]) in lipoproteins. We explore the possibilities of detecting alcohol biomarkers in non-high-density-lipoproteins (non-HDLs) precipitated from serum using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). Methods: Analyzes were carried out on stored serum samples, with known % carbohydrate-deficient transferrin (CDT) values, included in a driver's license regranting program under the control of the Belgian Institute of Road Safety. The study consisted of 127 control samples (CDT <= 1.3%) and 114 alcoholic samples (CDT > 1.3%). Liver enzymes, CRP, triglycerides, total, HDL- and LDL-cholesterol values were determined. Non-HDLs were precipitated with sodium phosphotungstate and MgCl2 and analyzed using ATR-FTIR in the range from 4500 cm(-1) to 450 cm(-1) using a Perkin Elmer ATR-FTIR Spectrometer Two. Results: The area under the curve of the 1130-990 cm(-1) region (AUC(1130-990 cm-1)) was able to discriminate controls from alcoholics (p < 0.0001) due to the presence of FAEEs in lipoproteins. Multiple regression analysis significantly predicted the AUC(1130-990 cm-1) (adj. r(2) = 0.13, p < 0.0001). Significant correlations were found between AUC(1130-990 cm-1) and CDT values (r = 0.32, p < 0.0001), AST/ALT ratio (r = 0.21, p = 0.001). GGT showed no significant correlation. Conclusions: Infrared analysis of lipoproteins is a potential tool in the detection of alcohol biomarkers.
Keywords
alcoholism, diagnostic markers, fatty acid ethyl esters, infrared spectroscopy, lipoproteins, CARBOHYDRATE-DEFICIENT TRANSFERRIN, PHOSPHATIDYLETHANOL PETH, ETHANOL METABOLITES, FT-IR, MARKERS, BLOOD, DRIVERS, PROGRAM

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 213.71 KB

Citation

Please use this url to cite or link to this publication:

Chicago
De Bruyne, Sander, Tinne Monteyne, Marijn Speeckaert, and Joris Delanghe. 2017. “Infrared Analysis of Lipoproteins in the Detection of Alcohol Biomarkers.” Clinical Chemistry and Laboratory Medicine 55 (6): 876–881.
APA
De Bruyne, Sander, Monteyne, T., Speeckaert, M., & Delanghe, J. (2017). Infrared analysis of lipoproteins in the detection of alcohol biomarkers. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 55(6), 876–881.
Vancouver
1.
De Bruyne S, Monteyne T, Speeckaert M, Delanghe J. Infrared analysis of lipoproteins in the detection of alcohol biomarkers. CLINICAL CHEMISTRY AND LABORATORY MEDICINE. 2017;55(6):876–81.
MLA
De Bruyne, Sander, Tinne Monteyne, Marijn Speeckaert, et al. “Infrared Analysis of Lipoproteins in the Detection of Alcohol Biomarkers.” CLINICAL CHEMISTRY AND LABORATORY MEDICINE 55.6 (2017): 876–881. Print.
@article{8519914,
  abstract     = {Background: Alcoholism is a major public health problem. Alcohol causes modifications in the composition and concentration of lipoproteins and influences the enzymes and transfer proteins that transform lipoproteins in plasma. Alcohol is associated with the presence of alcohol biomarkers (fatty acid ethyl esters [FAEEs] and phosphatidylethanol [PEth]) in lipoproteins. We explore the possibilities of detecting alcohol biomarkers in non-high-density-lipoproteins (non-HDLs) precipitated from serum using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). 
Methods: Analyzes were carried out on stored serum samples, with known \% carbohydrate-deficient transferrin (CDT) values, included in a driver's license regranting program under the control of the Belgian Institute of Road Safety. The study consisted of 127 control samples (CDT {\textlangle}= 1.3\%) and 114 alcoholic samples (CDT {\textrangle} 1.3\%). Liver enzymes, CRP, triglycerides, total, HDL- and LDL-cholesterol values were determined. Non-HDLs were precipitated with sodium phosphotungstate and MgCl2 and analyzed using ATR-FTIR in the range from 4500 cm(-1) to 450 cm(-1) using a Perkin Elmer ATR-FTIR Spectrometer Two. 
Results: The area under the curve of the 1130-990 cm(-1) region (AUC(1130-990 cm-1)) was able to discriminate controls from alcoholics (p {\textlangle} 0.0001) due to the presence of FAEEs in lipoproteins. Multiple regression analysis significantly predicted the AUC(1130-990 cm-1) (adj. r(2) = 0.13, p {\textlangle} 0.0001). Significant correlations were found between AUC(1130-990 cm-1) and CDT values (r = 0.32, p {\textlangle} 0.0001), AST/ALT ratio (r = 0.21, p = 0.001). GGT showed no significant correlation. 
Conclusions: Infrared analysis of lipoproteins is a potential tool in the detection of alcohol biomarkers.},
  author       = {De Bruyne, Sander and Monteyne, Tinne and Speeckaert, Marijn and Delanghe, Joris},
  issn         = {1434-6621},
  journal      = {CLINICAL CHEMISTRY AND LABORATORY MEDICINE},
  keyword      = {alcoholism,diagnostic markers,fatty acid ethyl esters,infrared spectroscopy,lipoproteins,CARBOHYDRATE-DEFICIENT TRANSFERRIN,PHOSPHATIDYLETHANOL PETH,ETHANOL METABOLITES,FT-IR,MARKERS,BLOOD,DRIVERS,PROGRAM},
  language     = {eng},
  number       = {6},
  pages        = {876--881},
  title        = {Infrared analysis of lipoproteins in the detection of alcohol biomarkers},
  url          = {http://dx.doi.org/10.1515/cclm-2016-0668},
  volume       = {55},
  year         = {2017},
}

Altmetric
View in Altmetric
Web of Science
Times cited: