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A deep nasopharyngeal swab versus nonendoscopic bronchoalveolar lavage for isolation of bacterial pathogens from preweaned calves with respiratory disease

Laura Van Driessche UGent, Bonnie Valgaeren, Linde Gille UGent, Filip Boyen UGent, Richard Ducatelle UGent, Freddy Haesebrouck UGent, Piet Deprez UGent and Bart Pardon UGent (2017) JOURNAL OF VETERINARY INTERNAL MEDICINE. 31(3). p.946-953
abstract
Background: Nonendoscopic bronchoalveolar lavage (BAL) is a practical alternative for a deep nasopharyngeal swab (DNS) to sample the airways of a large number of calves in a short period of time. The extent of commensal overgrowth and agreement of BAL with DNS culture results in preweaned calves are unknown. Objectives: To compare commensal overgrowth and bacterial culture results between DNS and BAL samples. Animals: A total of 183 preweaned calves (144 with bovine respiratory disease and 39 healthy animals). Methods: Cross-sectional study. Deep nasopharyngeal swab and BAL samples were taken from each calf and cultured to detect Pasteurellaceae and Mycoplasma bovis. Agreement and associations between culture results of DNS and BAL samples were determined by kappa statistics and logistic regression. Results: Bronchoalveolar lavage samples were less often polymicrobial, more frequently negative and yielded more pure cultures compared to DNS, leading to a clinically interpretable culture result in 79.2% of the cases compared to only in 31.2% of the DNS samples. Isolation rates were lower in healthy animals, but not different between DNS and BAL samples. Only Histophilus somni was more likely to be isolated from BAL samples. In clinical cases, a polymicrobial DNS culture result did not increase the probability of a polymicrobial BAL result by >= 30%, nor did it influence the probability of a negative culture. A significant herd effect was noted for all observed relationships. Conclusions and Clinical Relevance: Nonendoscopic BAL samples are far less overgrown by bacteria compared to DNS samples under the conditions of this study, facilitating clinical interpretation and resulting in a higher return on investment in bacteriologic culturing.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Bacteria, Bovine respiratory disease, Sampling, Comparison, VEAL CALVES, FEEDLOT CALVES, IMMUNOGLOBULIN, PASTEURELLA, HAEMOLYTICA, AGREEMENT, CATTLE, FLORA
journal title
JOURNAL OF VETERINARY INTERNAL MEDICINE
J. Vet. Intern. Med.
volume
31
issue
3
pages
946 - 953
Web of Science type
Article
Web of Science id
000405196800044
ISSN
1939-1676
DOI
10.1111/jvim.14668
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8519911
handle
http://hdl.handle.net/1854/LU-8519911
date created
2017-05-09 11:36:58
date last changed
2017-10-23 08:18:23
@article{8519911,
  abstract     = {Background: Nonendoscopic bronchoalveolar lavage (BAL) is a practical alternative for a deep nasopharyngeal swab (DNS) to sample the airways of a large number of calves in a short period of time. The extent of commensal overgrowth and agreement of BAL with DNS culture results in preweaned calves are unknown. 
Objectives: To compare commensal overgrowth and bacterial culture results between DNS and BAL samples. 
Animals: A total of 183 preweaned calves (144 with bovine respiratory disease and 39 healthy animals). 
Methods: Cross-sectional study. Deep nasopharyngeal swab and BAL samples were taken from each calf and cultured to detect Pasteurellaceae and Mycoplasma bovis. Agreement and associations between culture results of DNS and BAL samples were determined by kappa statistics and logistic regression. 
Results: Bronchoalveolar lavage samples were less often polymicrobial, more frequently negative and yielded more pure cultures compared to DNS, leading to a clinically interpretable culture result in 79.2\% of the cases compared to only in 31.2\% of the DNS samples. Isolation rates were lower in healthy animals, but not different between DNS and BAL samples. Only Histophilus somni was more likely to be isolated from BAL samples. In clinical cases, a polymicrobial DNS culture result did not increase the probability of a polymicrobial BAL result by {\textrangle}= 30\%, nor did it influence the probability of a negative culture. A significant herd effect was noted for all observed relationships. Conclusions and 
Clinical Relevance: Nonendoscopic BAL samples are far less overgrown by bacteria compared to DNS samples under the conditions of this study, facilitating clinical interpretation and resulting in a higher return on investment in bacteriologic culturing.},
  author       = {Van Driessche, Laura and Valgaeren, Bonnie and Gille, Linde and Boyen, Filip and Ducatelle, Richard and Haesebrouck, Freddy and Deprez, Piet and Pardon, Bart},
  issn         = {1939-1676},
  journal      = {JOURNAL OF VETERINARY INTERNAL MEDICINE},
  keyword      = {Bacteria,Bovine respiratory disease,Sampling,Comparison,VEAL CALVES,FEEDLOT CALVES,IMMUNOGLOBULIN,PASTEURELLA,HAEMOLYTICA,AGREEMENT,CATTLE,FLORA},
  language     = {eng},
  number       = {3},
  pages        = {946--953},
  title        = {A deep nasopharyngeal swab versus nonendoscopic bronchoalveolar lavage for isolation of bacterial pathogens from preweaned calves with respiratory disease},
  url          = {http://dx.doi.org/10.1111/jvim.14668},
  volume       = {31},
  year         = {2017},
}

Chicago
Van Driessche, Laura, Bonnie Valgaeren, Linde Gille, Filip Boyen, Richard Ducatelle, Freddy Haesebrouck, Piet Deprez, and Bart Pardon. 2017. “A Deep Nasopharyngeal Swab Versus Nonendoscopic Bronchoalveolar Lavage for Isolation of Bacterial Pathogens from Preweaned Calves with Respiratory Disease.” Journal of Veterinary Internal Medicine 31 (3): 946–953.
APA
Van Driessche, Laura, Valgaeren, B., Gille, L., Boyen, F., Ducatelle, R., Haesebrouck, F., Deprez, P., et al. (2017). A deep nasopharyngeal swab versus nonendoscopic bronchoalveolar lavage for isolation of bacterial pathogens from preweaned calves with respiratory disease. JOURNAL OF VETERINARY INTERNAL MEDICINE, 31(3), 946–953.
Vancouver
1.
Van Driessche L, Valgaeren B, Gille L, Boyen F, Ducatelle R, Haesebrouck F, et al. A deep nasopharyngeal swab versus nonendoscopic bronchoalveolar lavage for isolation of bacterial pathogens from preweaned calves with respiratory disease. JOURNAL OF VETERINARY INTERNAL MEDICINE. 2017;31(3):946–53.
MLA
Van Driessche, Laura, Bonnie Valgaeren, Linde Gille, et al. “A Deep Nasopharyngeal Swab Versus Nonendoscopic Bronchoalveolar Lavage for Isolation of Bacterial Pathogens from Preweaned Calves with Respiratory Disease.” JOURNAL OF VETERINARY INTERNAL MEDICINE 31.3 (2017): 946–953. Print.