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Effect of sample temperature, pH, and matrix on the percentage protein binding of protein-bound uraemic toxins

Olivier Deltombe (UGent) , Annemieke Dhondt (UGent) , Wim Van Biesen (UGent) , Griet Glorieux (UGent) and Sunny Eloot (UGent)
(2017) ANALYTICAL METHODS. 9(12). p.1935-1940
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Organization
Abstract
While studying and trying to optimise dialysis clearances of protein-bound uraemic toxins (PBUTs), the percentage protein binding (% PB) may be an important parameter and can be calculated from measured free and total concentrations. Since different parameters may alter this % PB, we investigated whether the ultrafiltration temperature, sample pH, and sample matrix (i. e. serum or plasma) affects the % PB of PBUTs. Pre-dialysis serum and plasma samples were obtained from 10 stable haemodialysis patients. Ultrafiltration was performed at 37 degrees C for fresh samples and at 4 degrees C, room temperature, and 37 degrees C for thawed samples (all n = 10). Total and free serum/ plasma concentrations of hippuric acid, indole-3-acetic acid, indoxyl sulphate, and p-cresylsulphate were simultaneously measured by high-performance liquid chromatography with ultraviolet and fluorescence detection. No differences in % PB were found between fresh and thawed samples at 37 degrees C or between serum and plasma samples prepared at the same temperatures. However, in both serum and plasma samples, the free concentration increased with increasing ultrafiltration temperatures and resulted in a decrease in % PB from 4 degrees C to 37 degrees C. In conclusion, the % PB of PBUTs can be determined in both thawed serum and plasma samples and ultrafiltration should be performed at 37 degrees C.
Keywords
CHRONIC KIDNEY-DISEASE, HUMAN SERUM-ALBUMIN, INDOXYL SULFATE, EQUILIBRIUM DIALYSIS, MASS-SPECTROMETRY, PLASMA, HEMODIALYSIS, CRESYLSULPHATE, SOLUTES, DRUGS

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MLA
Deltombe, Olivier, Annemieke Dhondt, Wim Van Biesen, et al. “Effect of Sample Temperature, pH, and Matrix on the Percentage Protein Binding of Protein-bound Uraemic Toxins.” ANALYTICAL METHODS 9.12 (2017): 1935–1940. Print.
APA
Deltombe, O., Dhondt, A., Van Biesen, W., Glorieux, G., & Eloot, S. (2017). Effect of sample temperature, pH, and matrix on the percentage protein binding of protein-bound uraemic toxins. ANALYTICAL METHODS, 9(12), 1935–1940.
Chicago author-date
Deltombe, Olivier, Annemieke Dhondt, Wim Van Biesen, Griet Glorieux, and Sunny Eloot. 2017. “Effect of Sample Temperature, pH, and Matrix on the Percentage Protein Binding of Protein-bound Uraemic Toxins.” Analytical Methods 9 (12): 1935–1940.
Chicago author-date (all authors)
Deltombe, Olivier, Annemieke Dhondt, Wim Van Biesen, Griet Glorieux, and Sunny Eloot. 2017. “Effect of Sample Temperature, pH, and Matrix on the Percentage Protein Binding of Protein-bound Uraemic Toxins.” Analytical Methods 9 (12): 1935–1940.
Vancouver
1.
Deltombe O, Dhondt A, Van Biesen W, Glorieux G, Eloot S. Effect of sample temperature, pH, and matrix on the percentage protein binding of protein-bound uraemic toxins. ANALYTICAL METHODS. 2017;9(12):1935–40.
IEEE
[1]
O. Deltombe, A. Dhondt, W. Van Biesen, G. Glorieux, and S. Eloot, “Effect of sample temperature, pH, and matrix on the percentage protein binding of protein-bound uraemic toxins,” ANALYTICAL METHODS, vol. 9, no. 12, pp. 1935–1940, 2017.
@article{8519609,
  abstract     = {While studying and trying to optimise dialysis clearances of protein-bound uraemic toxins (PBUTs), the percentage protein binding (% PB) may be an important parameter and can be calculated from measured free and total concentrations. Since different parameters may alter this % PB, we investigated whether the ultrafiltration temperature, sample pH, and sample matrix (i. e. serum or plasma) affects the % PB of PBUTs. Pre-dialysis serum and plasma samples were obtained from 10 stable haemodialysis patients. Ultrafiltration was performed at 37 degrees C for fresh samples and at 4 degrees C, room temperature, and 37 degrees C for thawed samples (all n = 10). Total and free serum/ plasma concentrations of hippuric acid, indole-3-acetic acid, indoxyl sulphate, and p-cresylsulphate were simultaneously measured by high-performance liquid chromatography with ultraviolet and fluorescence detection. No differences in % PB were found between fresh and thawed samples at 37 degrees C or between serum and plasma samples prepared at the same temperatures. However, in both serum and plasma samples, the free concentration increased with increasing ultrafiltration temperatures and resulted in a decrease in % PB from 4 degrees C to 37 degrees C. In conclusion, the % PB of PBUTs can be determined in both thawed serum and plasma samples and ultrafiltration should be performed at 37 degrees C.},
  author       = {Deltombe, Olivier and Dhondt, Annemieke and Van Biesen, Wim and Glorieux, Griet and Eloot, Sunny},
  issn         = {1759-9660},
  journal      = {ANALYTICAL METHODS},
  keywords     = {CHRONIC KIDNEY-DISEASE,HUMAN SERUM-ALBUMIN,INDOXYL SULFATE,EQUILIBRIUM DIALYSIS,MASS-SPECTROMETRY,PLASMA,HEMODIALYSIS,CRESYLSULPHATE,SOLUTES,DRUGS},
  language     = {eng},
  number       = {12},
  pages        = {1935--1940},
  title        = {Effect of sample temperature, pH, and matrix on the percentage protein binding of protein-bound uraemic toxins},
  url          = {http://dx.doi.org/10.1039/c7ay00054e},
  volume       = {9},
  year         = {2017},
}

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