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Aeromicrobium choanae sp. nov., an actinobacterium isolated from the choana of a garden warbler

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Abstract
A Gram-stain-positive, non-spore-forming actinobacterium, strain 9H-4(T), isolated from the choana of a garden warbler (Sylvia borin) was studied to examine its taxonomic position. On the basis of 16S rRNA gene sequence analysis it was shown that strain 9H-4(T) belongs to the genus Aeromicrobium with Aeromicrobium flavum TYLN1(T) (98.7% similarity) and Aeromicrobium tamlense SSW1-57(T) (98.4 %) as the nearest neighbours and forms a separate branch in a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences. DNA-DNA hybridizations confirmed its novel species identity based on 39 and 46% DNA-DNA relatedness with A. flavum DSM 19355(T) and A. tamlense DSM 19087(T), respectively. The predominant menaquinone of strain 9H-4(T) was MK-9(H-4). The peptidoglycan contained LL-diaminopimelic acid as the diagnostic diamino acid. The major fatty acids (C-18 : 1 omega 9c, 10-methyl C-18 : 0, C-16 : 0, C-18 : 0, C-16 : 0 2-OH) were consistent with the fatty acid patterns reported for members of the genus Aeromicrobium. The DNA G+C content of strain 9H-4(T) was 70.8 mol%. The distinct genotypic, chemotaxonomic, physiological and biochemical characteristics support the classification of strain 9H-4(T) as a representative of a novel species of the genus Aeromicrobium, for which the name Aeromicrobium choanae sp. nov. is proposed. The type strain is 9H-4(T) (=ZIM B1021(T)=LMG 29165(T)=CCM 8650(T)).
Keywords
AD-HOC-COMMITTEE, DEOXYRIBONUCLEIC-ACID, GENUS AEROMICROBIUM, GINSENG FIELD, IDENTIFICATION, ACETOBACTER, SEQUENCE, HYBRIDIZATION, SOIL

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Chicago
Ber, Polona, Stefanie Van Trappen, Peter Vandamme, and Janja Trček. 2017. “Aeromicrobium Choanae Sp. Nov., an Actinobacterium Isolated from the Choana of a Garden Warbler.” International Journal of Systematic and Evolutionary Microbiology 67 (2): 432–440.
APA
Ber, Polona, Van Trappen, S., Vandamme, P., & Trček, J. (2017). Aeromicrobium choanae sp. nov., an actinobacterium isolated from the choana of a garden warbler. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 67(2), 432–440.
Vancouver
1.
Ber P, Van Trappen S, Vandamme P, Trček J. Aeromicrobium choanae sp. nov., an actinobacterium isolated from the choana of a garden warbler. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2017;67(2):432–40.
MLA
Ber, Polona, Stefanie Van Trappen, Peter Vandamme, et al. “Aeromicrobium Choanae Sp. Nov., an Actinobacterium Isolated from the Choana of a Garden Warbler.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 67.2 (2017): 432–440. Print.
@article{8519512,
  abstract     = {A Gram-stain-positive, non-spore-forming actinobacterium, strain 9H-4(T), isolated from the choana of a garden warbler (Sylvia borin) was studied to examine its taxonomic position. On the basis of 16S rRNA gene sequence analysis it was shown that strain 9H-4(T) belongs to the genus Aeromicrobium with Aeromicrobium flavum TYLN1(T) (98.7\% similarity) and Aeromicrobium tamlense SSW1-57(T) (98.4 \%) as the nearest neighbours and forms a separate branch in a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences. DNA-DNA hybridizations confirmed its novel species identity based on 39 and 46\% DNA-DNA relatedness with A. flavum DSM 19355(T) and A. tamlense DSM 19087(T), respectively. The predominant menaquinone of strain 9H-4(T) was MK-9(H-4). The peptidoglycan contained LL-diaminopimelic acid as the diagnostic diamino acid. The major fatty acids (C-18 : 1 omega 9c, 10-methyl C-18 : 0, C-16 : 0, C-18 : 0, C-16 : 0 2-OH) were consistent with the fatty acid patterns reported for members of the genus Aeromicrobium. The DNA G+C content of strain 9H-4(T) was 70.8 mol\%. The distinct genotypic, chemotaxonomic, physiological and biochemical characteristics support the classification of strain 9H-4(T) as a representative of a novel species of the genus Aeromicrobium, for which the name Aeromicrobium choanae sp. nov. is proposed. The type strain is 9H-4(T) (=ZIM B1021(T)=LMG 29165(T)=CCM 8650(T)).},
  author       = {Ber, Polona and Van Trappen, Stefanie and Vandamme, Peter and Tr\v{c}ek, Janja},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {AD-HOC-COMMITTEE,DEOXYRIBONUCLEIC-ACID,GENUS AEROMICROBIUM,GINSENG FIELD,IDENTIFICATION,ACETOBACTER,SEQUENCE,HYBRIDIZATION,SOIL},
  language     = {eng},
  number       = {2},
  pages        = {432--440},
  title        = {Aeromicrobium choanae sp. nov., an actinobacterium isolated from the choana of a garden warbler},
  url          = {http://dx.doi.org/10.1099/ijsem.0.001632},
  volume       = {67},
  year         = {2017},
}

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