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Aeromicrobium choanae sp. nov., an actinobacterium isolated from the choana of a garden warbler

Polona Ber, Stefanie Van Trappen, Peter Vandamme UGent and Janja Trček (2017) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 67(2). p.432-440
abstract
A Gram-stain-positive, non-spore-forming actinobacterium, strain 9H-4(T), isolated from the choana of a garden warbler (Sylvia borin) was studied to examine its taxonomic position. On the basis of 16S rRNA gene sequence analysis it was shown that strain 9H-4(T) belongs to the genus Aeromicrobium with Aeromicrobium flavum TYLN1(T) (98.7% similarity) and Aeromicrobium tamlense SSW1-57(T) (98.4 %) as the nearest neighbours and forms a separate branch in a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences. DNA-DNA hybridizations confirmed its novel species identity based on 39 and 46% DNA-DNA relatedness with A. flavum DSM 19355(T) and A. tamlense DSM 19087(T), respectively. The predominant menaquinone of strain 9H-4(T) was MK-9(H-4). The peptidoglycan contained LL-diaminopimelic acid as the diagnostic diamino acid. The major fatty acids (C-18 : 1 omega 9c, 10-methyl C-18 : 0, C-16 : 0, C-18 : 0, C-16 : 0 2-OH) were consistent with the fatty acid patterns reported for members of the genus Aeromicrobium. The DNA G+C content of strain 9H-4(T) was 70.8 mol%. The distinct genotypic, chemotaxonomic, physiological and biochemical characteristics support the classification of strain 9H-4(T) as a representative of a novel species of the genus Aeromicrobium, for which the name Aeromicrobium choanae sp. nov. is proposed. The type strain is 9H-4(T) (=ZIM B1021(T)=LMG 29165(T)=CCM 8650(T)).
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
AD-HOC-COMMITTEE, DEOXYRIBONUCLEIC-ACID, GENUS AEROMICROBIUM, GINSENG FIELD, IDENTIFICATION, ACETOBACTER, SEQUENCE, HYBRIDIZATION, SOIL
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
67
issue
2
pages
432 - 440
Web of Science type
Article
Web of Science id
000397002800028
ISSN
1466-5026
1466-5034
DOI
10.1099/ijsem.0.001632
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8519512
handle
http://hdl.handle.net/1854/LU-8519512
date created
2017-05-04 08:43:32
date last changed
2017-05-30 12:33:02
@article{8519512,
  abstract     = {A Gram-stain-positive, non-spore-forming actinobacterium, strain 9H-4(T), isolated from the choana of a garden warbler (Sylvia borin) was studied to examine its taxonomic position. On the basis of 16S rRNA gene sequence analysis it was shown that strain 9H-4(T) belongs to the genus Aeromicrobium with Aeromicrobium flavum TYLN1(T) (98.7\% similarity) and Aeromicrobium tamlense SSW1-57(T) (98.4 \%) as the nearest neighbours and forms a separate branch in a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences. DNA-DNA hybridizations confirmed its novel species identity based on 39 and 46\% DNA-DNA relatedness with A. flavum DSM 19355(T) and A. tamlense DSM 19087(T), respectively. The predominant menaquinone of strain 9H-4(T) was MK-9(H-4). The peptidoglycan contained LL-diaminopimelic acid as the diagnostic diamino acid. The major fatty acids (C-18 : 1 omega 9c, 10-methyl C-18 : 0, C-16 : 0, C-18 : 0, C-16 : 0 2-OH) were consistent with the fatty acid patterns reported for members of the genus Aeromicrobium. The DNA G+C content of strain 9H-4(T) was 70.8 mol\%. The distinct genotypic, chemotaxonomic, physiological and biochemical characteristics support the classification of strain 9H-4(T) as a representative of a novel species of the genus Aeromicrobium, for which the name Aeromicrobium choanae sp. nov. is proposed. The type strain is 9H-4(T) (=ZIM B1021(T)=LMG 29165(T)=CCM 8650(T)).},
  author       = {Ber, Polona and Van Trappen, Stefanie and Vandamme, Peter and Tr\v{c}ek, Janja},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {AD-HOC-COMMITTEE,DEOXYRIBONUCLEIC-ACID,GENUS AEROMICROBIUM,GINSENG FIELD,IDENTIFICATION,ACETOBACTER,SEQUENCE,HYBRIDIZATION,SOIL},
  language     = {eng},
  number       = {2},
  pages        = {432--440},
  title        = {Aeromicrobium choanae sp. nov., an actinobacterium isolated from the choana of a garden warbler},
  url          = {http://dx.doi.org/10.1099/ijsem.0.001632},
  volume       = {67},
  year         = {2017},
}

Chicago
Ber, Polona, Stefanie Van Trappen, Peter Vandamme, and Janja Trček. 2017. “Aeromicrobium Choanae Sp. Nov., an Actinobacterium Isolated from the Choana of a Garden Warbler.” International Journal of Systematic and Evolutionary Microbiology 67 (2): 432–440.
APA
Ber, Polona, Van Trappen, S., Vandamme, P., & Trček, J. (2017). Aeromicrobium choanae sp. nov., an actinobacterium isolated from the choana of a garden warbler. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 67(2), 432–440.
Vancouver
1.
Ber P, Van Trappen S, Vandamme P, Trček J. Aeromicrobium choanae sp. nov., an actinobacterium isolated from the choana of a garden warbler. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2017;67(2):432–40.
MLA
Ber, Polona, Stefanie Van Trappen, Peter Vandamme, et al. “Aeromicrobium Choanae Sp. Nov., an Actinobacterium Isolated from the Choana of a Garden Warbler.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 67.2 (2017): 432–440. Print.