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A reassortant H9N2 influenza virus containing 2009 pandemic H1N1 internal-protein genes acquired enhanced pig-to-pig transmission after serial passages in swine

José Carlos Mancera Gracia, Silvie Van den Hoecke, Juergen A Richt, Wenjun Ma, Xavier Saelens UGent and Kristien Van Reeth UGent (2017) SCIENTIFIC REPORTS. 7.
abstract
Avian H9N2 and 2009 pandemic H1N1 (pH1N1) influenza viruses can infect pigs and humans, raising the concern that H9N2: pH1N1 reassortant viruses could emerge. Such reassortants demonstrated increased replication and transmissibility in pig, but were still inefficient when compared to pH1N1. Here, we evaluated if a reassortant virus containing the hemagglutinin and neuraminidase of A/quail/ Hong Kong/G1/1997 (H9N2) in the A/California/04/2009 (pH1N1) backbone could become better adapted to pigs by serial passaging. The tropism of the original H9N2: pH1N1 (P0) virus was restricted to the nasal mucosa, with no virus detected in the trachea or lungs. Nevertheless, after seven passages the H9N2: pH1N1 (P7) virus replicated in the entire respiratory tract. We also compared the transmissibility of H9N2: pH1N1 (P0), H9N2: pH1N1 (P7) and pH1N1. While only 2/6 direct-contact pigs showed nasal virus excretion of H9N2: pH1N1 (P0) >= five days, 4/6 direct-contact animals shed the H9N2: pH1N1 (P7). Interestingly, those four animals shed virus with titers similar to those of the pH1N1, which readily transmitted to all six contact animals. The broader tissue tropism and the increased post-transmission replication after seven passages were associated with the HA-D225G substitution. Our data demonstrate that the pH1N1 internal-protein genes together with the serial passages favour H9N2 virus adaptation to pigs.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
A VIRUSES, AVIAN H9N2, REPLICATION, CHINA, HEMAGGLUTININ, EVOLUTION, ADAPTATION, POLYMERASE, RECEPTORS, DIVERSITY
journal title
SCIENTIFIC REPORTS
Sci. Rep.
volume
7
article number
1323
pages
10 pages
Web of Science type
Article
Web of Science id
000400449000035
ISSN
2045-2322
DOI
10.1038/s41598-017-01512-x
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8519308
handle
http://hdl.handle.net/1854/LU-8519308
date created
2017-05-03 07:51:58
date last changed
2018-06-21 12:21:30
@article{8519308,
  abstract     = {Avian H9N2 and 2009 pandemic H1N1 (pH1N1) influenza viruses can infect pigs and humans, raising the concern that H9N2: pH1N1 reassortant viruses could emerge. Such reassortants demonstrated increased replication and transmissibility in pig, but were still inefficient when compared to pH1N1. Here, we evaluated if a reassortant virus containing the hemagglutinin and neuraminidase of A/quail/ Hong Kong/G1/1997 (H9N2) in the A/California/04/2009 (pH1N1) backbone could become better adapted to pigs by serial passaging. The tropism of the original H9N2: pH1N1 (P0) virus was restricted to the nasal mucosa, with no virus detected in the trachea or lungs. Nevertheless, after seven passages the H9N2: pH1N1 (P7) virus replicated in the entire respiratory tract. We also compared the transmissibility of H9N2: pH1N1 (P0), H9N2: pH1N1 (P7) and pH1N1. While only 2/6 direct-contact pigs showed nasal virus excretion of H9N2: pH1N1 (P0) {\textrangle}= five days, 4/6 direct-contact animals shed the H9N2: pH1N1 (P7). Interestingly, those four animals shed virus with titers similar to those of the pH1N1, which readily transmitted to all six contact animals. The broader tissue tropism and the increased post-transmission replication after seven passages were associated with the HA-D225G substitution. Our data demonstrate that the pH1N1 internal-protein genes together with the serial passages favour H9N2 virus adaptation to pigs.},
  articleno    = {1323},
  author       = {Mancera Gracia, Jos{\'e} Carlos and Van den Hoecke, Silvie and Richt, Juergen A and Ma, Wenjun and Saelens, Xavier and Van Reeth, Kristien},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keyword      = {A VIRUSES,AVIAN H9N2,REPLICATION,CHINA,HEMAGGLUTININ,EVOLUTION,ADAPTATION,POLYMERASE,RECEPTORS,DIVERSITY},
  language     = {eng},
  pages        = {10},
  title        = {A reassortant H9N2 influenza virus containing 2009 pandemic H1N1 internal-protein genes acquired enhanced pig-to-pig transmission after serial passages in swine},
  url          = {http://dx.doi.org/10.1038/s41598-017-01512-x},
  volume       = {7},
  year         = {2017},
}

Chicago
Mancera Gracia, José Carlos, Silvie Van den Hoecke, Juergen A Richt, Wenjun Ma, Xavier Saelens, and Kristien Van Reeth. 2017. “A Reassortant H9N2 Influenza Virus Containing 2009 Pandemic H1N1 Internal-protein Genes Acquired Enhanced Pig-to-pig Transmission After Serial Passages in Swine.” Scientific Reports 7.
APA
Mancera Gracia, J. C., Van den Hoecke, S., Richt, J. A., Ma, W., Saelens, X., & Van Reeth, K. (2017). A reassortant H9N2 influenza virus containing 2009 pandemic H1N1 internal-protein genes acquired enhanced pig-to-pig transmission after serial passages in swine. SCIENTIFIC REPORTS, 7.
Vancouver
1.
Mancera Gracia JC, Van den Hoecke S, Richt JA, Ma W, Saelens X, Van Reeth K. A reassortant H9N2 influenza virus containing 2009 pandemic H1N1 internal-protein genes acquired enhanced pig-to-pig transmission after serial passages in swine. SCIENTIFIC REPORTS. 2017;7.
MLA
Mancera Gracia, José Carlos, Silvie Van den Hoecke, Juergen A Richt, et al. “A Reassortant H9N2 Influenza Virus Containing 2009 Pandemic H1N1 Internal-protein Genes Acquired Enhanced Pig-to-pig Transmission After Serial Passages in Swine.” SCIENTIFIC REPORTS 7 (2017): n. pag. Print.