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A multiplexed microfluidic platform for bone marker measurement : a proof-of-concept

Patricia Khashayar (UGent) , Ghassem Amoabediny, Bagher Larijani, Morteza Hosseini, Rik Verplancke (UGent) , David Schaubroeck (UGent) , Steven Van Put (UGent) , Farideh Razi, Michel De Keersmaecker (UGent) , Mieke Adriaens (UGent) , et al.
(2017) MICROMACHINES. 8(5).
Author
Organization
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Abstract
In this work, we report a microfluidic platform that can be easily translated into a biomarker diagnostic. This platform integrates microfluidic technology with electrochemical sensing and embodies a reaction/detection chamber to measure serum levels of different biomarkers. Microfabricated Au electrodes encased in a microfluidic chamber are functionalized to immobilize the antibodies, which can selectively capture the corresponding antigen. An oxidative peak is obtained using the chronoamperometry technique at room temperature. The magnitude of the response current varies linearly with the logarithmic concentration of the relative biomarker and, thus, is used to quantify the concentration of the relative biomarker in serum samples. We demonstrated the implementation, feasibility and specificity of this platform (Osteokit) in assaying serum levels of bone turnover markers (BTMs) using osteocalcin (limits of detection (LOD) = 1.94 ng/mL) and collagen type 1 cross-linked C-telopeptide (CTX) (LOD = 1.39 pg/mL). To our knowledge, this is the first such device fabricated to measure BTMs. Our results also showed that the sensitivity of Osteokit is comparable with the current states of art, electrochemiluminescence (ECLIA).
Keywords
biosensor, bone turnover markers, osteoporosis, gold nanoparticles, electrochemistry, CANCER DIAGNOSTICS, POSTMENOPAUSAL WOMEN, BIOCHEMICAL MARKERS, PARATHYROID-HORMONE, MINERAL DENSITY, PROTEIN, OSTEOPOROSIS, TURNOVER, SYSTEMS, SURFACES

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Citation

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MLA
Khashayar, Patricia, et al. “A Multiplexed Microfluidic Platform for Bone Marker Measurement : A Proof-of-Concept.” MICROMACHINES, vol. 8, no. 5, 2017, doi:10.3390/mi8050133.
APA
Khashayar, P., Amoabediny, G., Larijani, B., Hosseini, M., Verplancke, R., Schaubroeck, D., … Vanfleteren, J. (2017). A multiplexed microfluidic platform for bone marker measurement : a proof-of-concept. MICROMACHINES, 8(5). https://doi.org/10.3390/mi8050133
Chicago author-date
Khashayar, Patricia, Ghassem Amoabediny, Bagher Larijani, Morteza Hosseini, Rik Verplancke, David Schaubroeck, Steven Van Put, et al. 2017. “A Multiplexed Microfluidic Platform for Bone Marker Measurement : A Proof-of-Concept.” MICROMACHINES 8 (5). https://doi.org/10.3390/mi8050133.
Chicago author-date (all authors)
Khashayar, Patricia, Ghassem Amoabediny, Bagher Larijani, Morteza Hosseini, Rik Verplancke, David Schaubroeck, Steven Van Put, Farideh Razi, Michel De Keersmaecker, Mieke Adriaens, Stefan Goemaere, Tom Fiers, and Jan Vanfleteren. 2017. “A Multiplexed Microfluidic Platform for Bone Marker Measurement : A Proof-of-Concept.” MICROMACHINES 8 (5). doi:10.3390/mi8050133.
Vancouver
1.
Khashayar P, Amoabediny G, Larijani B, Hosseini M, Verplancke R, Schaubroeck D, et al. A multiplexed microfluidic platform for bone marker measurement : a proof-of-concept. MICROMACHINES. 2017;8(5).
IEEE
[1]
P. Khashayar et al., “A multiplexed microfluidic platform for bone marker measurement : a proof-of-concept,” MICROMACHINES, vol. 8, no. 5, 2017.
@article{8518819,
  abstract     = {{In this work, we report a microfluidic platform that can be easily translated into a biomarker diagnostic. This platform integrates microfluidic technology with electrochemical sensing and embodies a reaction/detection chamber to measure serum levels of different biomarkers. Microfabricated Au electrodes encased in a microfluidic chamber are functionalized to immobilize the antibodies, which can selectively capture the corresponding antigen. An oxidative peak is obtained using the chronoamperometry technique at room temperature. The magnitude of the response current varies linearly with the logarithmic concentration of the relative biomarker and, thus, is used to quantify the concentration of the relative biomarker in serum samples. We demonstrated the implementation, feasibility and specificity of this platform (Osteokit) in assaying serum levels of bone turnover markers (BTMs) using osteocalcin (limits of detection (LOD) = 1.94 ng/mL) and collagen type 1 cross-linked C-telopeptide (CTX) (LOD = 1.39 pg/mL). To our knowledge, this is the first such device fabricated to measure BTMs. Our results also showed that the sensitivity of Osteokit is comparable with the current states of art, electrochemiluminescence (ECLIA).}},
  articleno    = {{133}},
  author       = {{Khashayar, Patricia and Amoabediny, Ghassem and Larijani, Bagher and Hosseini, Morteza and Verplancke, Rik and Schaubroeck, David and Van Put, Steven and Razi, Farideh and De Keersmaecker, Michel and Adriaens, Mieke and Goemaere, Stefan and Fiers, Tom and Vanfleteren, Jan}},
  issn         = {{2072-666X}},
  journal      = {{MICROMACHINES}},
  keywords     = {{biosensor,bone turnover markers,osteoporosis,gold nanoparticles,electrochemistry,CANCER DIAGNOSTICS,POSTMENOPAUSAL WOMEN,BIOCHEMICAL MARKERS,PARATHYROID-HORMONE,MINERAL DENSITY,PROTEIN,OSTEOPOROSIS,TURNOVER,SYSTEMS,SURFACES}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{13}},
  title        = {{A multiplexed microfluidic platform for bone marker measurement : a proof-of-concept}},
  url          = {{http://doi.org/10.3390/mi8050133}},
  volume       = {{8}},
  year         = {{2017}},
}
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