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Dietary supplement based on stilbenes : a focus on gut microbial metabolism by the in vitro simulator M-SHIME®

(2016) FOOD & FUNCTION. 7(11). p.4564-4575
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Abstract
Polyphenols and intestinal microbiota can influence each other, modifying metabolism and gut wellness. Data on this mutual effect need to be improved. Several studies on the biological activities of resveratrol and derivatives have been carried out, but the effects of a continuous administration of stilbenes on gut microbiota have not yet been investigated. This study evaluated the effects of an extract from Vitis vinifera, containing a combination of t-resveratrol and epsilon-viniferin, on intestinal microbiota, using the advanced gastrointestinal simulator M-SHIME (R). A triple M-SHIME (R) experiment was performed using two concentrations of the extract (i.e. 1 and 2 g L-1), simulating a continuous daily intake. The effects were evaluated in terms of microbial functionality (SCFA and NH4+) and composition (DGGE and Illumina sequencing), since the microbiological aspect has been less considered so far. The treatment induced changes in microbial functionality and composition. In fact, the levels of SCFA and NH4+ suffered a strong decrease (i.e. inhibition of the saccharolytic and proteolytic activity), while DGGE and Illumina showed important modifications of the microbiota composition, associated with an imbalance of the colonic microbiota (i. e. increase in the relative abundance of Enterobacteriaceae). HPLC-DAD-TOF-MS analyses demonstrated that the metabolism of t-resveratrol and other stilbenes was inhibited by continuous administration. Our results suggest M-SHIME (R) as an explorative tool to define the dosage of food supplements, in particular to simulate effective continuous administration in humans.
Keywords
GRADIENT GEL-ELECTROPHORESIS, 16S RIBOSOMAL-RNA, ACTIVATED-SLUDGE, RESVERATROL, POLYPHENOLS, COMMUNITY, BIOAVAILABILITY, MODEL, PCR, BIOAUGMENTATION

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Chicago
Giuliani, Camilla, Massimo Marzorati, Marzia Innocenti, Ramiro Vilchez Vargas, Marius Vital, Dietmar H Pieper, Tom Van de Wiele, and Nadia Mulinacci. 2016. “Dietary Supplement Based on Stilbenes : a Focus on Gut Microbial Metabolism by the in Vitro Simulator M-SHIME®.” Food & Function 7 (11): 4564–4575.
APA
Giuliani, C., Marzorati, M., Innocenti, M., Vilchez Vargas, R., Vital, M., Pieper, D. H., Van de Wiele, T., et al. (2016). Dietary supplement based on stilbenes : a focus on gut microbial metabolism by the in vitro simulator M-SHIME®. FOOD & FUNCTION, 7(11), 4564–4575.
Vancouver
1.
Giuliani C, Marzorati M, Innocenti M, Vilchez Vargas R, Vital M, Pieper DH, et al. Dietary supplement based on stilbenes : a focus on gut microbial metabolism by the in vitro simulator M-SHIME®. FOOD & FUNCTION. 2016;7(11):4564–75.
MLA
Giuliani, Camilla, Massimo Marzorati, Marzia Innocenti, et al. “Dietary Supplement Based on Stilbenes : a Focus on Gut Microbial Metabolism by the in Vitro Simulator M-SHIME®.” FOOD & FUNCTION 7.11 (2016): 4564–4575. Print.
@article{8518638,
  abstract     = {Polyphenols and intestinal microbiota can influence each other, modifying metabolism and gut wellness. Data on this mutual effect need to be improved. Several studies on the biological activities of resveratrol and derivatives have been carried out, but the effects of a continuous administration of stilbenes on gut microbiota have not yet been investigated. This study evaluated the effects of an extract from Vitis vinifera, containing a combination of t-resveratrol and epsilon-viniferin, on intestinal microbiota, using the advanced gastrointestinal simulator M-SHIME (R). A triple M-SHIME (R) experiment was performed using two concentrations of the extract (i.e. 1 and 2 g L-1), simulating a continuous daily intake. The effects were evaluated in terms of microbial functionality (SCFA and NH4+) and composition (DGGE and Illumina sequencing), since the microbiological aspect has been less considered so far. The treatment induced changes in microbial functionality and composition. In fact, the levels of SCFA and NH4+ suffered a strong decrease (i.e. inhibition of the saccharolytic and proteolytic activity), while DGGE and Illumina showed important modifications of the microbiota composition, associated with an imbalance of the colonic microbiota (i. e. increase in the relative abundance of Enterobacteriaceae). HPLC-DAD-TOF-MS analyses demonstrated that the metabolism of t-resveratrol and other stilbenes was inhibited by continuous administration. Our results suggest M-SHIME (R) as an explorative tool to define the dosage of food supplements, in particular to simulate effective continuous administration in humans.},
  author       = {Giuliani, Camilla and Marzorati, Massimo and Innocenti, Marzia and Vilchez Vargas, Ramiro and Vital, Marius and Pieper, Dietmar H and Van de Wiele, Tom and Mulinacci, Nadia},
  issn         = {2042-6496},
  journal      = {FOOD \& FUNCTION},
  keyword      = {GRADIENT GEL-ELECTROPHORESIS,16S RIBOSOMAL-RNA,ACTIVATED-SLUDGE,RESVERATROL,POLYPHENOLS,COMMUNITY,BIOAVAILABILITY,MODEL,PCR,BIOAUGMENTATION},
  language     = {eng},
  number       = {11},
  pages        = {4564--4575},
  title        = {Dietary supplement based on stilbenes : a focus on gut microbial metabolism by the in vitro simulator M-SHIME{\textregistered}},
  url          = {http://dx.doi.org/10.1039/c6fo00784h},
  volume       = {7},
  year         = {2016},
}

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