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Dietary supplement based on stilbenes : a focus on gut microbial metabolism by the in vitro simulator M-SHIME®

Camilla Giuliani, Massimo Marzorati UGent, Marzia Innocenti, Ramiro Vilchez Vargas, Marius Vital, Dietmar H Pieper, Tom Van de Wiele UGent and Nadia Mulinacci (2016) FOOD & FUNCTION. 7(11). p.4564-4575
abstract
Polyphenols and intestinal microbiota can influence each other, modifying metabolism and gut wellness. Data on this mutual effect need to be improved. Several studies on the biological activities of resveratrol and derivatives have been carried out, but the effects of a continuous administration of stilbenes on gut microbiota have not yet been investigated. This study evaluated the effects of an extract from Vitis vinifera, containing a combination of t-resveratrol and epsilon-viniferin, on intestinal microbiota, using the advanced gastrointestinal simulator M-SHIME (R). A triple M-SHIME (R) experiment was performed using two concentrations of the extract (i.e. 1 and 2 g L-1), simulating a continuous daily intake. The effects were evaluated in terms of microbial functionality (SCFA and NH4+) and composition (DGGE and Illumina sequencing), since the microbiological aspect has been less considered so far. The treatment induced changes in microbial functionality and composition. In fact, the levels of SCFA and NH4+ suffered a strong decrease (i.e. inhibition of the saccharolytic and proteolytic activity), while DGGE and Illumina showed important modifications of the microbiota composition, associated with an imbalance of the colonic microbiota (i. e. increase in the relative abundance of Enterobacteriaceae). HPLC-DAD-TOF-MS analyses demonstrated that the metabolism of t-resveratrol and other stilbenes was inhibited by continuous administration. Our results suggest M-SHIME (R) as an explorative tool to define the dosage of food supplements, in particular to simulate effective continuous administration in humans.
Please use this url to cite or link to this publication:
author
organization
alternative title
Dietary supplement based on stilbenes : a focus on gut microbial metabolism by the in vitro simulator M-SHIME (R)
year
type
journalArticle (original)
publication status
published
subject
keyword
GRADIENT GEL-ELECTROPHORESIS, 16S RIBOSOMAL-RNA, ACTIVATED-SLUDGE, RESVERATROL, POLYPHENOLS, COMMUNITY, BIOAVAILABILITY, MODEL, PCR, BIOAUGMENTATION
journal title
FOOD & FUNCTION
Food Funct.
volume
7
issue
11
pages
4564 - 4575
Web of Science type
Article
Web of Science id
000388363000009
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
3.247 (2016)
JCR rank
15/129 (2016)
JCR quartile
1 (2016)
ISSN
2042-6496
2042-650X
DOI
10.1039/c6fo00784h
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8518638
handle
http://hdl.handle.net/1854/LU-8518638
date created
2017-04-25 12:59:50
date last changed
2018-04-12 12:54:37
@article{8518638,
  abstract     = {Polyphenols and intestinal microbiota can influence each other, modifying metabolism and gut wellness. Data on this mutual effect need to be improved. Several studies on the biological activities of resveratrol and derivatives have been carried out, but the effects of a continuous administration of stilbenes on gut microbiota have not yet been investigated. This study evaluated the effects of an extract from Vitis vinifera, containing a combination of t-resveratrol and epsilon-viniferin, on intestinal microbiota, using the advanced gastrointestinal simulator M-SHIME (R). A triple M-SHIME (R) experiment was performed using two concentrations of the extract (i.e. 1 and 2 g L-1), simulating a continuous daily intake. The effects were evaluated in terms of microbial functionality (SCFA and NH4+) and composition (DGGE and Illumina sequencing), since the microbiological aspect has been less considered so far. The treatment induced changes in microbial functionality and composition. In fact, the levels of SCFA and NH4+ suffered a strong decrease (i.e. inhibition of the saccharolytic and proteolytic activity), while DGGE and Illumina showed important modifications of the microbiota composition, associated with an imbalance of the colonic microbiota (i. e. increase in the relative abundance of Enterobacteriaceae). HPLC-DAD-TOF-MS analyses demonstrated that the metabolism of t-resveratrol and other stilbenes was inhibited by continuous administration. Our results suggest M-SHIME (R) as an explorative tool to define the dosage of food supplements, in particular to simulate effective continuous administration in humans.},
  author       = {Giuliani, Camilla and Marzorati, Massimo and Innocenti, Marzia and Vilchez Vargas, Ramiro and Vital, Marius and Pieper, Dietmar H and Van de Wiele, Tom and Mulinacci, Nadia},
  issn         = {2042-6496},
  journal      = {FOOD \& FUNCTION},
  keyword      = {GRADIENT GEL-ELECTROPHORESIS,16S RIBOSOMAL-RNA,ACTIVATED-SLUDGE,RESVERATROL,POLYPHENOLS,COMMUNITY,BIOAVAILABILITY,MODEL,PCR,BIOAUGMENTATION},
  language     = {eng},
  number       = {11},
  pages        = {4564--4575},
  title        = {Dietary supplement based on stilbenes : a focus on gut microbial metabolism by the in vitro simulator M-SHIME{\textregistered}},
  url          = {http://dx.doi.org/10.1039/c6fo00784h},
  volume       = {7},
  year         = {2016},
}

Chicago
Giuliani, Camilla, Massimo Marzorati, Marzia Innocenti, Ramiro Vilchez Vargas, Marius Vital, Dietmar H Pieper, Tom Van de Wiele, and Nadia Mulinacci. 2016. “Dietary Supplement Based on Stilbenes : a Focus on Gut Microbial Metabolism by the in Vitro Simulator M-SHIME®.” Food & Function 7 (11): 4564–4575.
APA
Giuliani, C., Marzorati, M., Innocenti, M., Vilchez Vargas, R., Vital, M., Pieper, D. H., Van de Wiele, T., et al. (2016). Dietary supplement based on stilbenes : a focus on gut microbial metabolism by the in vitro simulator M-SHIME®. FOOD & FUNCTION, 7(11), 4564–4575.
Vancouver
1.
Giuliani C, Marzorati M, Innocenti M, Vilchez Vargas R, Vital M, Pieper DH, et al. Dietary supplement based on stilbenes : a focus on gut microbial metabolism by the in vitro simulator M-SHIME®. FOOD & FUNCTION. 2016;7(11):4564–75.
MLA
Giuliani, Camilla, Massimo Marzorati, Marzia Innocenti, et al. “Dietary Supplement Based on Stilbenes : a Focus on Gut Microbial Metabolism by the in Vitro Simulator M-SHIME®.” FOOD & FUNCTION 7.11 (2016): 4564–4575. Print.