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T84 monolayers are superior to Caco-2 as a model system of colonocytes

Sarah Devriese (UGent) , Lien Van den Bossche (UGent) , Sophie Van Welden (UGent) , Tom Holvoet (UGent) , Iris Pinheiro, Pieter Hindryckx (UGent) , Martine De Vos (UGent) and Debby Laukens (UGent)
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Abstract
Colonic adenocarcinoma-derived Caco-2 and T84 epithelial cell lines are frequently used as in vitro model systems of functional epithelial barriers. Both are utilised interchangeably despite evidence that differentiated Caco-2 cells are more reminiscent of small intestinal enterocytes than of colonocytes, whereas differentiated T84 cells are less well characterised. The aim of this study was, therefore, to further characterise and compare differentiated Caco-2 and T84 cells. The objectives were to (1) compare the brush border morphology, (2) measure the expression of enterocyte- and colonocyte-specific genes and (3) compare their response to butyrate, which is dependent on the monocarboxylate transporter 1 (MCT1), an apical protein expressed primarily in colonocytes. T84 microvilli were significantly shorter than those of Caco-2 cells, which is a characteristic difference between small intestinal enterocytes and colonocytes. Also, enterocyte-associated brush border enzymes expressed in differentiated Caco-2 cells were not increased during T84 maturation, whereas colonic markers such as MCT1 were more abundant in differentiated T84 cells compared to differentiated Caco-2 cells. Consequently, T84 cells displayed a dose-responsive improvement of barrier function towards butyrate, which was absent in Caco-2 cells. On the other hand, differences in epithelial toll-like receptor expression between Caco-2 and T84 monolayers did not result in a corresponding differential functional response. We conclude that differentiated Caco-2 and T84 cells have distinct morphological, biochemical and functional characteristics, suggesting that T84 cells do not acquire the biochemical signature of mature small intestinal enterocytes like Caco-2 cells, but retain much of their original colonic characteristics throughout differentiation. These findings can help investigators select the appropriate intestinal epithelial cell line for specific in vitro research purposes.
Keywords
Colonocyte, Enterocyte, Differentiation, Epithelial cells, MCT1, INTESTINAL BARRIER, CELL-LINE, HUMAN-COLON, BACTERIAL LIPOPOLYSACCHARIDE, ENTEROCYTIC DIFFERENTIATION, BUTYRATE, EXPRESSION, CULTURE, TRANSPORT, DISEASE

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Citation

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MLA
Devriese, Sarah et al. “T84 Monolayers Are Superior to Caco-2 as a Model System of Colonocytes.” HISTOCHEMISTRY AND CELL BIOLOGY 148.1 (2017): 85–93. Print.
APA
Devriese, S., Van den Bossche, L., Van Welden, S., Holvoet, T., Pinheiro, I., Hindryckx, P., De Vos, M., et al. (2017). T84 monolayers are superior to Caco-2 as a model system of colonocytes. HISTOCHEMISTRY AND CELL BIOLOGY, 148(1), 85–93.
Chicago author-date
Devriese, Sarah, Lien Van den Bossche, Sophie Van Welden, Tom Holvoet, Iris Pinheiro, Pieter Hindryckx, Martine De Vos, and Debby Laukens. 2017. “T84 Monolayers Are Superior to Caco-2 as a Model System of Colonocytes.” Histochemistry and Cell Biology 148 (1): 85–93.
Chicago author-date (all authors)
Devriese, Sarah, Lien Van den Bossche, Sophie Van Welden, Tom Holvoet, Iris Pinheiro, Pieter Hindryckx, Martine De Vos, and Debby Laukens. 2017. “T84 Monolayers Are Superior to Caco-2 as a Model System of Colonocytes.” Histochemistry and Cell Biology 148 (1): 85–93.
Vancouver
1.
Devriese S, Van den Bossche L, Van Welden S, Holvoet T, Pinheiro I, Hindryckx P, et al. T84 monolayers are superior to Caco-2 as a model system of colonocytes. HISTOCHEMISTRY AND CELL BIOLOGY. 2017;148(1):85–93.
IEEE
[1]
S. Devriese et al., “T84 monolayers are superior to Caco-2 as a model system of colonocytes,” HISTOCHEMISTRY AND CELL BIOLOGY, vol. 148, no. 1, pp. 85–93, 2017.
@article{8518632,
  abstract     = {Colonic adenocarcinoma-derived Caco-2 and T84 epithelial cell lines are frequently used as in vitro model systems of functional epithelial barriers. Both are utilised interchangeably despite evidence that differentiated Caco-2 cells are more reminiscent of small intestinal enterocytes than of colonocytes, whereas differentiated T84 cells are less well characterised. The aim of this study was, therefore, to further characterise and compare differentiated Caco-2 and T84 cells. The objectives were to (1) compare the brush border morphology, (2) measure the expression of enterocyte- and colonocyte-specific genes and (3) compare their response to butyrate, which is dependent on the monocarboxylate transporter 1 (MCT1), an apical protein expressed primarily in colonocytes. T84 microvilli were significantly shorter than those of Caco-2 cells, which is a characteristic difference between small intestinal enterocytes and colonocytes. Also, enterocyte-associated brush border enzymes expressed in differentiated Caco-2 cells were not increased during T84 maturation, whereas colonic markers such as MCT1 were more abundant in differentiated T84 cells compared to differentiated Caco-2 cells. Consequently, T84 cells displayed a dose-responsive improvement of barrier function towards butyrate, which was absent in Caco-2 cells. On the other hand, differences in epithelial toll-like receptor expression between Caco-2 and T84 monolayers did not result in a corresponding differential functional response. We conclude that differentiated Caco-2 and T84 cells have distinct morphological, biochemical and functional characteristics, suggesting that T84 cells do not acquire the biochemical signature of mature small intestinal enterocytes like Caco-2 cells, but retain much of their original colonic characteristics throughout differentiation. These findings can help investigators select the appropriate intestinal epithelial cell line for specific in vitro research purposes.},
  author       = {Devriese, Sarah and Van den Bossche, Lien and Van Welden, Sophie and Holvoet, Tom and Pinheiro, Iris and Hindryckx, Pieter and De Vos, Martine and Laukens, Debby},
  issn         = {0948-6143},
  journal      = {HISTOCHEMISTRY AND CELL BIOLOGY},
  keywords     = {Colonocyte,Enterocyte,Differentiation,Epithelial cells,MCT1,INTESTINAL BARRIER,CELL-LINE,HUMAN-COLON,BACTERIAL LIPOPOLYSACCHARIDE,ENTEROCYTIC DIFFERENTIATION,BUTYRATE,EXPRESSION,CULTURE,TRANSPORT,DISEASE},
  language     = {eng},
  number       = {1},
  pages        = {85--93},
  title        = {T84 monolayers are superior to Caco-2 as a model system of colonocytes},
  url          = {http://dx.doi.org/10.1007/s00418-017-1539-7},
  volume       = {148},
  year         = {2017},
}

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