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Effects of different sterilization methods on the physico-chemical and bioresponsive properties of plasma-treated polycaprolactone films

Rouba Ghobeira (UGent) , Charlot Philips (UGent) , Heidi Declercq (UGent) , Pieter Cools (UGent) , Nathalie De Geyter (UGent) , Maria Cornelissen (UGent) and Rino Morent (UGent)
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Abstract
For most tissue engineering applications, surface modification and sterilization of polymers are critical aspects determining the implant success. The first part of this study is thus dedicated to modifying polycaprolactone (PCL) surfaces via plasma treatment using a medium pressure dielectric barrier discharge, while the second part focuses on the sterilization of plasma-modified PCL. Chemical and physical surface changes are examined making use of water contact angle goniometry (WCA), x-ray photoelectron spectroscopy and atomic force microscopy. Bioresponsive properties are evaluated by performing cell culture tests. The results show that air and argon plasmas decrease the WCA significantly due to the incorporation of oxygen-containing functionalities onto the PCL surface, without modifying its morphology. Extended treatment times lead to PCL degradation, especially in the case of air plasma. In addition to surface modification, the plasma potential to sterilize PCL is studied with appropriate treatment times, but sterility has not been achieved so far. Therefore, plasmamodified films are subjected to UV, H2O2 plasma (HP) and ethylene oxide (EtO) sterilizations. UV exposure of 3 h does not alter the PCL physico-chemical properties. A decreased wettability is observed after EtO sterilization, attributable to the modification of PCL chain ends reacting with EtO molecules. HP sterilization increases the WCA of the plasma-treated samples, presumably due to the scission of the hydrophilic bonds generated during the prior plasma treatments. Moreover, HP modifies the PCL surface morphology. For all the sterilizations, an improved cell adhesion and proliferation is observed on plasma-treated films compared to untreated ones. EtO shows the lowest proliferation rate compared to HP and UV. Overall, of the three sterilizations, UV is the most effective, since the physical alterations provoked by HP might interfere with the structural integrity when it comes to 3D scaffolds, and the chemical modifications caused by EtO, in addition to its toxicity, interfere with PCL bioactivity.

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Citation

Please use this url to cite or link to this publication:

MLA
Ghobeira, Rouba et al. “Effects of Different Sterilization Methods on the Physico-chemical and Bioresponsive Properties of Plasma-treated Polycaprolactone Films.” BIOMEDICAL MATERIALS 12.1 (2017): n. pag. Print.
APA
Ghobeira, R., Philips, C., Declercq, H., Cools, P., De Geyter, N., Cornelissen, M., & Morent, R. (2017). Effects of different sterilization methods on the physico-chemical and bioresponsive properties of plasma-treated polycaprolactone films. BIOMEDICAL MATERIALS, 12(1).
Chicago author-date
Ghobeira, Rouba, Charlot Philips, Heidi Declercq, Pieter Cools, Nathalie De Geyter, Maria Cornelissen, and Rino Morent. 2017. “Effects of Different Sterilization Methods on the Physico-chemical and Bioresponsive Properties of Plasma-treated Polycaprolactone Films.” Biomedical Materials 12 (1).
Chicago author-date (all authors)
Ghobeira, Rouba, Charlot Philips, Heidi Declercq, Pieter Cools, Nathalie De Geyter, Maria Cornelissen, and Rino Morent. 2017. “Effects of Different Sterilization Methods on the Physico-chemical and Bioresponsive Properties of Plasma-treated Polycaprolactone Films.” Biomedical Materials 12 (1).
Vancouver
1.
Ghobeira R, Philips C, Declercq H, Cools P, De Geyter N, Cornelissen M, et al. Effects of different sterilization methods on the physico-chemical and bioresponsive properties of plasma-treated polycaprolactone films. BIOMEDICAL MATERIALS. 2017;12(1).
IEEE
[1]
R. Ghobeira et al., “Effects of different sterilization methods on the physico-chemical and bioresponsive properties of plasma-treated polycaprolactone films,” BIOMEDICAL MATERIALS, vol. 12, no. 1, 2017.
@article{8518630,
  abstract     = {For most tissue engineering applications, surface modification and sterilization of polymers are critical aspects determining the implant success. The first part of this study is thus dedicated to modifying polycaprolactone (PCL) surfaces via plasma treatment using a medium pressure dielectric barrier discharge, while the second part focuses on the sterilization of plasma-modified PCL. Chemical and physical surface changes are examined making use of water contact angle goniometry (WCA), x-ray photoelectron spectroscopy and atomic force microscopy. Bioresponsive properties are evaluated by performing cell culture tests. The results show that air and argon plasmas decrease the WCA significantly due to the incorporation of oxygen-containing functionalities onto the PCL surface, without modifying its morphology. Extended treatment times lead to PCL degradation, especially in the case of air plasma. In addition to surface modification, the plasma potential to sterilize PCL is studied with appropriate treatment times, but sterility has not been achieved so far. Therefore, plasmamodified films are subjected to UV, H2O2 plasma (HP) and ethylene oxide (EtO) sterilizations. UV exposure of 3 h does not alter the PCL physico-chemical properties. A decreased wettability is observed after EtO sterilization, attributable to the modification of PCL chain ends reacting with EtO molecules. HP sterilization increases the WCA of the plasma-treated samples, presumably due to the scission of the hydrophilic bonds generated during the prior plasma treatments. Moreover, HP modifies the PCL surface morphology. For all the sterilizations, an improved cell adhesion and proliferation is observed on plasma-treated films compared to untreated ones. EtO shows the lowest proliferation rate compared to HP and UV. Overall, of the three sterilizations, UV is the most effective, since the physical alterations provoked by HP might interfere with the structural integrity when it comes to 3D scaffolds, and the chemical modifications caused by EtO, in addition to its toxicity, interfere with PCL bioactivity.},
  articleno    = {015017},
  author       = {Ghobeira, Rouba and Philips, Charlot and Declercq, Heidi and Cools, Pieter and De Geyter, Nathalie and Cornelissen, Maria and Morent, Rino},
  issn         = {1748-6041},
  journal      = {BIOMEDICAL MATERIALS},
  language     = {eng},
  number       = {1},
  pages        = {19},
  title        = {Effects of different sterilization methods on the physico-chemical and bioresponsive properties of plasma-treated polycaprolactone films},
  url          = {http://dx.doi.org/10.1088/1748-605x/aa51d5},
  volume       = {12},
  year         = {2017},
}

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