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Proteome profiling of wheat shoots from different cultivars

Lam Dai Vu (UGent) , Inge Verstraeten (UGent) , Elisabeth Stes (UGent) , Michiel Van Bel (UGent) , Frederik Coppens (UGent) , Kris Gevaert (UGent) and Ive De Smet (UGent)
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Abstract
Wheat is a cereal grain and one of the world's major food crops. Recent advances in wheat genome sequencing are by now facilitating its genomic and proteomic analyses. However, little is known about possible differences in total protein levels of hexaploid versus tetraploid wheat cultivars, and also knowledge of phosphorylated wheat proteins is still limited. Here, we performed a detailed analysis of the proteome of seedling leaves from two hexaploid wheat cultivars (Triticum aestivum L. Pavon 76 and USU-Apogee) and one tetraploid wheat (T. turgidum ssp. durum cv. Senatore Cappelli). Our shotgun proteomics data revealed that, whereas we observed some significant differences, overall a high similarity between hexaploid and tetraploid varieties with respect to protein abundance was observed. In addition, already at the seedling stage, a small set of proteins was differential between the small (USU-Apogee) and larger hexaploid wheat cultivars (Pavon 76), which could potentially act as growth predictors. Finally, the phosphosites identified in this study can be retrieved from the in-house developed plant PTM-Viewer (bioinformatics.psb.ugent.be/webtools/ptm_viewer/), making this the first searchable repository for phosphorylated wheat proteins. This paves the way for further in depth, quantitative (phospho) proteome-wide differential analyses upon a specific trigger or environmental change.
Keywords
TRITICUM-AESTIVUM L., BREAD WHEAT, QUANTITATIVE PHOSPHOPROTEOMICS, MASS-SPECTROMETRY, PLANT PROTEOMICS, SEEDLING LEAVES, HEXAPLOID WHEAT, SALT STRESS, TOLERANCE, PROTEINS, wheat, proteome, phosphoproteome, mass spectrometry, leaf

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Chicago
Vu, Lam Dai, Inge Verstraeten, Elisabeth Stes, Michiel Van Bel, Frederik Coppens, Kris Gevaert, and Ive De Smet. 2017. “Proteome Profiling of Wheat Shoots from Different Cultivars.” Frontiers in Plant Science 8.
APA
Vu, L. D., Verstraeten, I., Stes, E., Van Bel, M., Coppens, F., Gevaert, K., & De Smet, I. (2017). Proteome profiling of wheat shoots from different cultivars. FRONTIERS IN PLANT SCIENCE, 8.
Vancouver
1.
Vu LD, Verstraeten I, Stes E, Van Bel M, Coppens F, Gevaert K, et al. Proteome profiling of wheat shoots from different cultivars. FRONTIERS IN PLANT SCIENCE. 2017;8.
MLA
Vu, Lam Dai, Inge Verstraeten, Elisabeth Stes, et al. “Proteome Profiling of Wheat Shoots from Different Cultivars.” FRONTIERS IN PLANT SCIENCE 8 (2017): n. pag. Print.
@article{8518217,
  abstract     = {Wheat is a cereal grain and one of the world's major food crops. Recent advances in wheat genome sequencing are by now facilitating its genomic and proteomic analyses. However, little is known about possible differences in total protein levels of hexaploid versus tetraploid wheat cultivars, and also knowledge of phosphorylated wheat proteins is still limited. Here, we performed a detailed analysis of the proteome of seedling leaves from two hexaploid wheat cultivars (Triticum aestivum L. Pavon 76 and USU-Apogee) and one tetraploid wheat (T. turgidum ssp. durum cv. Senatore Cappelli). Our shotgun proteomics data revealed that, whereas we observed some significant differences, overall a high similarity between hexaploid and tetraploid varieties with respect to protein abundance was observed. In addition, already at the seedling stage, a small set of proteins was differential between the small (USU-Apogee) and larger hexaploid wheat cultivars (Pavon 76), which could potentially act as growth predictors. Finally, the phosphosites identified in this study can be retrieved from the in-house developed plant PTM-Viewer (bioinformatics.psb.ugent.be/webtools/ptm\_viewer/), making this the first searchable repository for phosphorylated wheat proteins. This paves the way for further in depth, quantitative (phospho) proteome-wide differential analyses upon a specific trigger or environmental change.},
  articleno    = {332},
  author       = {Vu, Lam Dai and Verstraeten, Inge and Stes, Elisabeth and Van Bel, Michiel and Coppens, Frederik and Gevaert, Kris and De Smet, Ive},
  issn         = {1664-462X},
  journal      = {FRONTIERS IN PLANT SCIENCE},
  language     = {eng},
  pages        = {11},
  title        = {Proteome profiling of wheat shoots from different cultivars},
  url          = {http://dx.doi.org/10.3389/fpls.2017.00332},
  volume       = {8},
  year         = {2017},
}

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