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Forever young : the role of ubiquitin receptor DA1 and E3 ligase BIG BROTHER in controlling leaf growth and development

Hannes Vanhaeren UGent, Younjeong Nam, Liesbeth De Milde UGent, Eunyoung Chae, Veronique Storme UGent, Detlef Weigel, Nathalie Gonzalez Sanchez and Dirk Inzé UGent (2017) PLANT PHYSIOLOGY. 173(2). p.1269-1282
abstract
The final size of plant organs is determined by a combination of cell proliferation and cell expansion. Leaves account for a large part of above-ground biomass and provide energy to complete the plant's life cycle. Although the final size of leaves is remarkably constant under fixed environmental conditions, several genes have been described to enhance leaf growth when their expression is modulated. In Arabidopsis (Arabidopsis thaliana), mutations in DA1 and BB increase leaf size, an effect that is synergistically enhanced in the double mutant. Here, we show that overexpression of a dominant-negative version of DA1 enhances leaf size in a broad range of natural accessions of this species, indicating a highly conserved role of this protein in controlling organ size. We also found that during early stages of development, leaves of da1-1 and bb/eod1-2 mutants were already larger than the isogenic Col-0 wild type, but this phenotype was triggered by different cellular mechanisms. Later during development, da1-1 and bb/eod1-2 leaves showed a prolonged longevity, which was enhanced in the double mutant. Conversely, ectopic expression of DA1 or BB restricted growth and promoted leaf senescence. In concert, shortly upon induction of DA1 and BB expression, several marker genes for the transition from proliferation to expansion were highly up-regulated. Additionally, multiple genes involved in maintaining the mitotic cell cycle were rapidly down-regulated and senescence genes were strongly up-regulated, particularly upon BB induction. With these results, we demonstrate that DA1 and BB restrict leaf size and promote senescence through converging and different mechanisms.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ARABIDOPSIS-THALIANA, ORGAN SIZE, TRANSCRIPTION FACTORS, DEVELOPING, LEAVES, NATURAL VARIATION, GENE FAMILY, SMALL AUXIN, SENESCENCE, EXPRESSION, LONGEVITY
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
173
issue
2
pages
1269 - 1282
Web of Science type
Article
Web of Science id
000394140800027
ISSN
0032-0889
1532-2548
DOI
10.1104/pp.16.01410
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8518214
handle
http://hdl.handle.net/1854/LU-8518214
date created
2017-04-20 11:39:55
date last changed
2017-07-03 10:41:14
@article{8518214,
  abstract     = {The final size of plant organs is determined by a combination of cell proliferation and cell expansion. Leaves account for a large part of above-ground biomass and provide energy to complete the plant's life cycle. Although the final size of leaves is remarkably constant under fixed environmental conditions, several genes have been described to enhance leaf growth when their expression is modulated. In Arabidopsis (Arabidopsis thaliana), mutations in DA1 and BB increase leaf size, an effect that is synergistically enhanced in the double mutant. Here, we show that overexpression of a dominant-negative version of DA1 enhances leaf size in a broad range of natural accessions of this species, indicating a highly conserved role of this protein in controlling organ size. We also found that during early stages of development, leaves of da1-1 and bb/eod1-2 mutants were already larger than the isogenic Col-0 wild type, but this phenotype was triggered by different cellular mechanisms. Later during development, da1-1 and bb/eod1-2 leaves showed a prolonged longevity, which was enhanced in the double mutant. Conversely, ectopic expression of DA1 or BB restricted growth and promoted leaf senescence. In concert, shortly upon induction of DA1 and BB expression, several marker genes for the transition from proliferation to expansion were highly up-regulated. Additionally, multiple genes involved in maintaining the mitotic cell cycle were rapidly down-regulated and senescence genes were strongly up-regulated, particularly upon BB induction. With these results, we demonstrate that DA1 and BB restrict leaf size and promote senescence through converging and different mechanisms.},
  author       = {Vanhaeren, Hannes and Nam, Younjeong and De Milde, Liesbeth and Chae, Eunyoung and Storme, Veronique and Weigel, Detlef and Gonzalez Sanchez, Nathalie and Inz{\'e}, Dirk},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {ARABIDOPSIS-THALIANA,ORGAN SIZE,TRANSCRIPTION FACTORS,DEVELOPING,LEAVES,NATURAL VARIATION,GENE FAMILY,SMALL AUXIN,SENESCENCE,EXPRESSION,LONGEVITY},
  language     = {eng},
  number       = {2},
  pages        = {1269--1282},
  title        = {Forever young : the role of ubiquitin receptor DA1 and E3 ligase BIG BROTHER in controlling leaf growth and development},
  url          = {http://dx.doi.org/10.1104/pp.16.01410},
  volume       = {173},
  year         = {2017},
}

Chicago
Vanhaeren, Hannes, Younjeong Nam, Liesbeth De Milde, Eunyoung Chae, Veronique Storme, Detlef Weigel, Nathalie Gonzalez Sanchez, and Dirk Inzé. 2017. “Forever Young : the Role of Ubiquitin Receptor DA1 and E3 Ligase BIG BROTHER in Controlling Leaf Growth and Development.” Plant Physiology 173 (2): 1269–1282.
APA
Vanhaeren, H., Nam, Y., De Milde, L., Chae, E., Storme, V., Weigel, D., Gonzalez Sanchez, N., et al. (2017). Forever young : the role of ubiquitin receptor DA1 and E3 ligase BIG BROTHER in controlling leaf growth and development. PLANT PHYSIOLOGY, 173(2), 1269–1282.
Vancouver
1.
Vanhaeren H, Nam Y, De Milde L, Chae E, Storme V, Weigel D, et al. Forever young : the role of ubiquitin receptor DA1 and E3 ligase BIG BROTHER in controlling leaf growth and development. PLANT PHYSIOLOGY. 2017;173(2):1269–82.
MLA
Vanhaeren, Hannes, Younjeong Nam, Liesbeth De Milde, et al. “Forever Young : the Role of Ubiquitin Receptor DA1 and E3 Ligase BIG BROTHER in Controlling Leaf Growth and Development.” PLANT PHYSIOLOGY 173.2 (2017): 1269–1282. Print.