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Silencing CHALCONE SYNTHASE in maize impedes the incorporation of tricin into lignin and increases lignin content

Nubia Barbosa Eloy, Wannes Voorend, Wu Lan, Marina Saleme de Lyra Soriano UGent, Igor Cesarino, Ruben Vanholme UGent, Rebecca Smith, Geert Goeminne UGent, Andreas Pallidis, Kris Morreel UGent, et al. (2017) PLANT PHYSIOLOGY. 173(2). p.998-1016
abstract
Lignin is a phenolic heteropolymer that is deposited in secondary-thickened cell walls, where it provides mechanical strength. A recent structural characterization of cell walls from monocot species showed that the flavone tricin is part of the native lignin polymer, where it is hypothesized to initiate lignin chains. In this study, we investigated the consequences of altered tricin levels on lignin structure and cell wall recalcitrance by phenolic profiling, nuclear magnetic resonance, and saccharification assays of the naturally silenced maize (Zea mays) C2-Idf (inhibitor diffuse) mutant, defective in the CHALCONE SYNTHASE Colorless2 (C2) gene. We show that the C2-Idf mutant produces highly reduced levels of apigenin-and tricin-related flavonoids, resulting in a strongly reduced incorporation of tricin into the lignin polymer. Moreover, the lignin was enriched in beta-beta and beta-5 units, lending support to the contention that tricin acts to initiate lignin chains and that, in the absence of tricin, more monolignol dimerization reactions occur. In addition, the C2-Idf mutation resulted in strikingly higher Klason lignin levels in the leaves. As a consequence, the leaves of C2-Idf mutants had significantly reduced saccharification efficiencies compared with those of control plants. These findings are instructive for lignin engineering strategies to improve biomass processing and biochemical production.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CELL-WALL COMPOSITION, ZEA-MAYS L, CAFFEOYL SHIKIMATE ESTERASE, PHENYLALANINE-AMMONIA-LYASE, FERMENTABLE SUGAR YIELDS, ACID O-METHYLTRANSFERASE, PANICUM-VIRGATUM L., COBRA-LIKE PROTEIN, ARABIDOPSIS-THALIANA, DOWN-REGULATION
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
173
issue
2
pages
998 - 1016
Web of Science type
Article
Web of Science id
000394140800008
ISSN
0032-0889
1532-2548
DOI
10.1104/pp.16.01108
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8518199
handle
http://hdl.handle.net/1854/LU-8518199
date created
2017-04-20 10:54:42
date last changed
2017-07-03 10:08:36
@article{8518199,
  abstract     = {Lignin is a phenolic heteropolymer that is deposited in secondary-thickened cell walls, where it provides mechanical strength. A recent structural characterization of cell walls from monocot species showed that the flavone tricin is part of the native lignin polymer, where it is hypothesized to initiate lignin chains. In this study, we investigated the consequences of altered tricin levels on lignin structure and cell wall recalcitrance by phenolic profiling, nuclear magnetic resonance, and saccharification assays of the naturally silenced maize (Zea mays) C2-Idf (inhibitor diffuse) mutant, defective in the CHALCONE SYNTHASE Colorless2 (C2) gene. We show that the C2-Idf mutant produces highly reduced levels of apigenin-and tricin-related flavonoids, resulting in a strongly reduced incorporation of tricin into the lignin polymer. Moreover, the lignin was enriched in beta-beta and beta-5 units, lending support to the contention that tricin acts to initiate lignin chains and that, in the absence of tricin, more monolignol dimerization reactions occur. In addition, the C2-Idf mutation resulted in strikingly higher Klason lignin levels in the leaves. As a consequence, the leaves of C2-Idf mutants had significantly reduced saccharification efficiencies compared with those of control plants. These findings are instructive for lignin engineering strategies to improve biomass processing and biochemical production.},
  author       = {Eloy, Nubia Barbosa and Voorend, Wannes and Lan, Wu and Saleme de Lyra Soriano, Marina and Cesarino, Igor and Vanholme, Ruben and Smith, Rebecca and Goeminne, Geert and Pallidis, Andreas and Morreel, Kris and Nicomedes, Jos{\'e} Junior and Ralph, John and Boerjan, Wout},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {CELL-WALL COMPOSITION,ZEA-MAYS L,CAFFEOYL SHIKIMATE ESTERASE,PHENYLALANINE-AMMONIA-LYASE,FERMENTABLE SUGAR YIELDS,ACID O-METHYLTRANSFERASE,PANICUM-VIRGATUM L.,COBRA-LIKE PROTEIN,ARABIDOPSIS-THALIANA,DOWN-REGULATION},
  language     = {eng},
  number       = {2},
  pages        = {998--1016},
  title        = {Silencing CHALCONE SYNTHASE in maize impedes the incorporation of tricin into lignin and increases lignin content},
  url          = {http://dx.doi.org/10.1104/pp.16.01108},
  volume       = {173},
  year         = {2017},
}

Chicago
Eloy, Nubia Barbosa, Wannes Voorend, Wu Lan, Marina de Lyra Soriano Saleme, Igor Cesarino, Ruben Vanholme, Rebecca Smith, et al. 2017. “Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content.” Plant Physiology 173 (2): 998–1016.
APA
Eloy, N. B., Voorend, W., Lan, W., de Lyra Soriano Saleme, M., Cesarino, I., Vanholme, R., Smith, R., et al. (2017). Silencing CHALCONE SYNTHASE in maize impedes the incorporation of tricin into lignin and increases lignin content. PLANT PHYSIOLOGY, 173(2), 998–1016.
Vancouver
1.
Eloy NB, Voorend W, Lan W, de Lyra Soriano Saleme M, Cesarino I, Vanholme R, et al. Silencing CHALCONE SYNTHASE in maize impedes the incorporation of tricin into lignin and increases lignin content. PLANT PHYSIOLOGY. 2017;173(2):998–1016.
MLA
Eloy, Nubia Barbosa, Wannes Voorend, Wu Lan, et al. “Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content.” PLANT PHYSIOLOGY 173.2 (2017): 998–1016. Print.