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The mutation nrpb1-A325V in the largest subunit of RNA polymerase II suppresses compromised growth of Arabidopsis plants deficient in a function of the general transcription factor IIF

Elena Babiychuk UGent, Hoang Khai Trinh UGent, Klaas Vandepoele UGent, Eveline Van De Slijke UGent, Danny Geelen UGent, Geert De Jaeger UGent, Junichi Obokata and Sergei Kushnir UGent (2017) PLANT JOURNAL. 89(4). p.730-745
abstract
The evolutionarily conserved 12-subunit RNA polymerase II (Pol II) is a central catalytic component that drives RNA synthesis during the transcription cycle that consists of transcription initiation, elongation, and termination. A diverse set of general transcription factors, including a multifunctional TFIIF, govern Pol II selectivity, kinetic properties, and transcription coupling with posttranscriptional processes. Here, we show that TFIIF of Arabidopsis (Arabidopsis thaliana) resembles the metazoan complex that is composed of the TFIIF and TFIIF polypeptides. Arabidopsis has two TFIIF subunits, of which TFIIF1/MAN1 is essential and TFIIF2/MAN2 is not. In the partial loss-of-function mutant allele man1-1, the winged helix domain of Arabidopsis TFIIF1/MAN1 was dispensable for plant viability, whereas the cellular organization of the shoot and root apical meristems were abnormal. Forward genetic screening identified an epistatic interaction between the largest Pol II subunit nrpb1-A325V variant and the man1-1 mutation. The suppression of the man1-1 mutant developmental defects by a mutation in Pol II suggests a link between TFIIF functions in Arabidopsis transcription cycle and the maintenance of cellular organization in the shoot and root apical meristems.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
START SITE UTILIZATION, DNA-BINDING DOMAIN, PREINITIATION COMPLEX, ROOT-MERISTEM, SACCHAROMYCES-CEREVISIAE, CELLULAR-ORGANIZATION, STRUCTURAL BASIS, TERMINAL DOMAIN, HIGH-RESOLUTION, CORE PROMOTER, Arabidopsis thaliana, TFIIF, RNA polymerase II, stem cells, meristem, cell identity, At4g12610, At1g75510, At3g52270, At4g35800
journal title
PLANT JOURNAL
Plant J.
volume
89
issue
4
pages
730 - 745
Web of Science type
Article
Web of Science id
000395802500007
ISSN
0960-7412
DOI
10.1111/tpj.13417
project
Bioinformatics: from nucleotids to networks (N2N)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8518186
handle
http://hdl.handle.net/1854/LU-8518186
date created
2017-04-20 10:03:36
date last changed
2017-08-02 09:53:12
@article{8518186,
  abstract     = {The evolutionarily conserved 12-subunit RNA polymerase II (Pol II) is a central catalytic component that drives RNA synthesis during the transcription cycle that consists of transcription initiation, elongation, and termination. A diverse set of general transcription factors, including a multifunctional TFIIF, govern Pol II selectivity, kinetic properties, and transcription coupling with posttranscriptional processes. Here, we show that TFIIF of Arabidopsis (Arabidopsis thaliana) resembles the metazoan complex that is composed of the TFIIF and TFIIF polypeptides. Arabidopsis has two TFIIF subunits, of which TFIIF1/MAN1 is essential and TFIIF2/MAN2 is not. In the partial loss-of-function mutant allele man1-1, the winged helix domain of Arabidopsis TFIIF1/MAN1 was dispensable for plant viability, whereas the cellular organization of the shoot and root apical meristems were abnormal. Forward genetic screening identified an epistatic interaction between the largest Pol II subunit nrpb1-A325V variant and the man1-1 mutation. The suppression of the man1-1 mutant developmental defects by a mutation in Pol II suggests a link between TFIIF functions in Arabidopsis transcription cycle and the maintenance of cellular organization in the shoot and root apical meristems.},
  author       = {Babiychuk, Elena and Trinh, Hoang Khai and Vandepoele, Klaas and Van De Slijke, Eveline and Geelen, Danny and De Jaeger, Geert and Obokata, Junichi and Kushnir, Sergei},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {START SITE UTILIZATION,DNA-BINDING DOMAIN,PREINITIATION COMPLEX,ROOT-MERISTEM,SACCHAROMYCES-CEREVISIAE,CELLULAR-ORGANIZATION,STRUCTURAL BASIS,TERMINAL DOMAIN,HIGH-RESOLUTION,CORE PROMOTER,Arabidopsis thaliana,TFIIF,RNA polymerase II,stem cells,meristem,cell identity,At4g12610,At1g75510,At3g52270,At4g35800},
  language     = {eng},
  number       = {4},
  pages        = {730--745},
  title        = {The mutation nrpb1-A325V in the largest subunit of RNA polymerase II suppresses compromised growth of Arabidopsis plants deficient in a function of the general transcription factor IIF},
  url          = {http://dx.doi.org/10.1111/tpj.13417},
  volume       = {89},
  year         = {2017},
}

Chicago
Babiychuk, Elena, Hoang Khai Trinh, Klaas Vandepoele, Eveline Van De Slijke, Danny Geelen, Geert De Jaeger, Junichi Obokata, and Sergei Kushnir. 2017. “The Mutation nrpb1-A325V in the Largest Subunit of RNA Polymerase II Suppresses Compromised Growth of Arabidopsis Plants Deficient in a Function of the General Transcription Factor IIF.” Plant Journal 89 (4): 730–745.
APA
Babiychuk, E., Trinh, H. K., Vandepoele, K., Van De Slijke, E., Geelen, D., De Jaeger, G., Obokata, J., et al. (2017). The mutation nrpb1-A325V in the largest subunit of RNA polymerase II suppresses compromised growth of Arabidopsis plants deficient in a function of the general transcription factor IIF. PLANT JOURNAL, 89(4), 730–745.
Vancouver
1.
Babiychuk E, Trinh HK, Vandepoele K, Van De Slijke E, Geelen D, De Jaeger G, et al. The mutation nrpb1-A325V in the largest subunit of RNA polymerase II suppresses compromised growth of Arabidopsis plants deficient in a function of the general transcription factor IIF. PLANT JOURNAL. 2017;89(4):730–45.
MLA
Babiychuk, Elena, Hoang Khai Trinh, Klaas Vandepoele, et al. “The Mutation nrpb1-A325V in the Largest Subunit of RNA Polymerase II Suppresses Compromised Growth of Arabidopsis Plants Deficient in a Function of the General Transcription Factor IIF.” PLANT JOURNAL 89.4 (2017): 730–745. Print.