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High-fiber and high-protein diets shape different gut microbial communities, which ecologically behave similarly under stress conditions, as shown in a gastrointestinal simulator

Massimo Marzorati UGent, Ramiro Vilchez Vargas, Julie Vanden Bussche UGent, Pilar Truchado, Ruy Jauregui, Racha El Hage UGent, Dietmar H Pieper, Lynn Vanhaecke UGent and Tom Van de Wiele UGent (2017) MOLECULAR NUTRITION & FOOD RESEARCH. 61(1).
abstract
The aim of this work was to investigate the relationship between the structure of gut microbial communities fed with different diets (i.e. high-protein-HP-versus high-fiber-HF-diet) and their functional stability when challenged with mild and acute doses of a mix of amoxicillin, ciprofloxacin, and tetracycline. We made use of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME (R))-a continuous model of the gastrointestinal tract-coupled with 16S-targeted Illumina and metabolomics (i.e. UHPLC-HRMS) analyses. Independently of the diet, the sudden exposure to an acute stress led to a modification of the microbial community structure, selecting for species belonging to Bacillus spp.; Clostridium cluster XIVa; Enterococci; Bacteroides; and Enterobacteriaceae. The antibiotic treatment led to a decrease in the number of operational taxonomic units (at least -10%). Cluster analysis of untargeted metabolic data showed that the antibiotic treatment affected the microbial activity. The impact on metabolites production was lower when the community was preexposed to mild doses of the antibiotic mix. This effect was stronger in the proximal colon for the HF diet and in the distal colon for the HP diet. Different diets shaped different gut microbial communities, which ecologically behaved similarly under stress conditions.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CHAIN FATTY-ACIDS, INTESTINAL MICROBIOTA, ECOSYSTEM, DISEASE, REGION, MODEL, COLON, RESILIENCE, MODULATION, STABILITY, Antibiotic, Functionality, Gastrointestinal tract, SHIME (R), Stress
journal title
MOLECULAR NUTRITION & FOOD RESEARCH
Mol. Nutr. Food Res.
volume
61
issue
1
article number
1600150
pages
13 pages
Web of Science type
Article
Web of Science id
000394841100014
ISSN
1613-4125
DOI
10.1002/mnfr.201600150
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8518117
handle
http://hdl.handle.net/1854/LU-8518117
date created
2017-04-19 13:14:52
date last changed
2017-06-22 09:24:31
@article{8518117,
  abstract     = {The aim of this work was to investigate the relationship between the structure of gut microbial communities fed with different diets (i.e. high-protein-HP-versus high-fiber-HF-diet) and their functional stability when challenged with mild and acute doses of a mix of amoxicillin, ciprofloxacin, and tetracycline. We made use of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME (R))-a continuous model of the gastrointestinal tract-coupled with 16S-targeted Illumina and metabolomics (i.e. UHPLC-HRMS) analyses. Independently of the diet, the sudden exposure to an acute stress led to a modification of the microbial community structure, selecting for species belonging to Bacillus spp.; Clostridium cluster XIVa; Enterococci; Bacteroides; and Enterobacteriaceae. The antibiotic treatment led to a decrease in the number of operational taxonomic units (at least -10\%). Cluster analysis of untargeted metabolic data showed that the antibiotic treatment affected the microbial activity. The impact on metabolites production was lower when the community was preexposed to mild doses of the antibiotic mix. This effect was stronger in the proximal colon for the HF diet and in the distal colon for the HP diet. Different diets shaped different gut microbial communities, which ecologically behaved similarly under stress conditions.},
  articleno    = {1600150},
  author       = {Marzorati, Massimo and Vilchez Vargas, Ramiro and Vanden Bussche, Julie and Truchado, Pilar and Jauregui, Ruy and El Hage, Racha and Pieper, Dietmar H and Vanhaecke, Lynn and Van de Wiele, Tom},
  issn         = {1613-4125},
  journal      = {MOLECULAR NUTRITION \& FOOD RESEARCH},
  keyword      = {CHAIN FATTY-ACIDS,INTESTINAL MICROBIOTA,ECOSYSTEM,DISEASE,REGION,MODEL,COLON,RESILIENCE,MODULATION,STABILITY,Antibiotic,Functionality,Gastrointestinal tract,SHIME (R),Stress},
  language     = {eng},
  number       = {1},
  pages        = {13},
  title        = {High-fiber and high-protein diets shape different gut microbial communities, which ecologically behave similarly under stress conditions, as shown in a gastrointestinal simulator},
  url          = {http://dx.doi.org/10.1002/mnfr.201600150},
  volume       = {61},
  year         = {2017},
}

Chicago
Marzorati, Massimo, Ramiro Vilchez Vargas, Julie Vanden Bussche, Pilar Truchado, Ruy Jauregui, Racha El Hage, Dietmar H Pieper, Lynn Vanhaecke, and Tom Van de Wiele. 2017. “High-fiber and High-protein Diets Shape Different Gut Microbial Communities, Which Ecologically Behave Similarly Under Stress Conditions, as Shown in a Gastrointestinal Simulator.” Molecular Nutrition & Food Research 61 (1).
APA
Marzorati, M., Vilchez Vargas, R., Vanden Bussche, J., Truchado, P., Jauregui, R., El Hage, R., Pieper, D. H., et al. (2017). High-fiber and high-protein diets shape different gut microbial communities, which ecologically behave similarly under stress conditions, as shown in a gastrointestinal simulator. MOLECULAR NUTRITION & FOOD RESEARCH, 61(1).
Vancouver
1.
Marzorati M, Vilchez Vargas R, Vanden Bussche J, Truchado P, Jauregui R, El Hage R, et al. High-fiber and high-protein diets shape different gut microbial communities, which ecologically behave similarly under stress conditions, as shown in a gastrointestinal simulator. MOLECULAR NUTRITION & FOOD RESEARCH. 2017;61(1).
MLA
Marzorati, Massimo, Ramiro Vilchez Vargas, Julie Vanden Bussche, et al. “High-fiber and High-protein Diets Shape Different Gut Microbial Communities, Which Ecologically Behave Similarly Under Stress Conditions, as Shown in a Gastrointestinal Simulator.” MOLECULAR NUTRITION & FOOD RESEARCH 61.1 (2017): n. pag. Print.