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Evaluation of combination therapy for Burkholderia cenocepacia lung infection in different in vitro and in vivo models

Freija Van den Driessche, Bieke Vanhoutte, Gilles Brackman, Aurélie Crabbé UGent, Petra Rigole UGent, Jurgen Vercruysse, Glenn Verstraete UGent, Davie Cappoen, Chris Vervaet UGent, Paul Cos, et al. (2017) PLOS ONE. 12(3).
abstract
Burkholderia cenocepacia is an opportunistic pathogen responsible for life-threatening infections in cystic fibrosis patients. B. cenocepacia is extremely resistant towards antibiotics and therapy is complicated by its ability to form biofilms. We investigated the efficacy of an alternative antimicrobial strategy for B. cenocepacia lung infections using in vitro and in vivo models. A screening of the NIH Clinical Collection 1&2 was performed against B. cenocepacia biofilms formed in 96-well microtiter plates in the presence of tobramycin to identify repurposing candidates with potentiator activity. The efficacy of selected hits was evaluated in a three-dimensional (3D) organotypic human lung epithelial cell culture model. The in vivo effect was evaluated in the invertebrate Galleria mellonella and in a murine B. cenocepacia lung infection model. The screening resulted in 60 hits that potentiated the activity of tobramycin against B. cenocepacia biofilms, including four imidazoles of which econazole and miconazole were selected for further investigation. However, a potentiator effect was not observed in the 3D organotypic human lung epithelial cell culture model. Combination treatment was also not able to increase survival of infected G. mellonella. Also in mice, there was no added value for the combination treatment. Although potentiators of tobramycin with activity against biofilms of B. cenocepacia were identified in a repurposing screen, the in vitro activity could not be confirmed nor in a more sophisticated in vitro model, neither in vivo. This stresses the importance of validating hits resulting from in vitro studies in physiologically relevant model systems.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HOST-PATHOGEN INTERACTIONS, CYSTIC-FIBROSIS PATIENTS, CEPACIA COMPLEX, CELL-CULTURE, PSEUDOMONAS-AERUGINOSA, STAPHYLOCOCCUS-AUREUS, ANTIBIOTIC-RESISTANCE, MICROBIAL BIOFILMS, EPITHELIAL-CELLS, MICONAZOLE
journal title
PLOS ONE
PLoS One
volume
12
issue
3
article number
e0172723
pages
16 pages
Web of Science type
Article
Web of Science id
000395983500053
ISSN
1932-6203
DOI
10.1371/journal.pone.0172723
language
English
UGent publication?
yes
classification
A1
additional info
the first two authors contributed equally to this work
copyright statement
I have retained and own the full copyright for this publication
id
8517760
handle
http://hdl.handle.net/1854/LU-8517760
date created
2017-04-14 09:52:20
date last changed
2017-04-18 08:57:33
@article{8517760,
  abstract     = {Burkholderia cenocepacia is an opportunistic pathogen responsible for life-threatening infections in cystic fibrosis patients. B. cenocepacia is extremely resistant towards antibiotics and therapy is complicated by its ability to form biofilms. We investigated the efficacy of an alternative antimicrobial strategy for B. cenocepacia lung infections using in vitro and in vivo models. A screening of the NIH Clinical Collection 1\&2 was performed against B. cenocepacia biofilms formed in 96-well microtiter plates in the presence of tobramycin to identify repurposing candidates with potentiator activity. The efficacy of selected hits was evaluated in a three-dimensional (3D) organotypic human lung epithelial cell culture model. The in vivo effect was evaluated in the invertebrate Galleria mellonella and in a murine B. cenocepacia lung infection model. The screening resulted in 60 hits that potentiated the activity of tobramycin against B. cenocepacia biofilms, including four imidazoles of which econazole and miconazole were selected for further investigation. However, a potentiator effect was not observed in the 3D organotypic human lung epithelial cell culture model. Combination treatment was also not able to increase survival of infected G. mellonella. Also in mice, there was no added value for the combination treatment. Although potentiators of tobramycin with activity against biofilms of B. cenocepacia were identified in a repurposing screen, the in vitro activity could not be confirmed nor in a more sophisticated in vitro model, neither in vivo. This stresses the importance of validating hits resulting from in vitro studies in physiologically relevant model systems.},
  articleno    = {e0172723},
  author       = {Van den Driessche, Freija and Vanhoutte, Bieke and Brackman, Gilles and Crabb{\'e}, Aur{\'e}lie and Rigole, Petra and Vercruysse, Jurgen and Verstraete, Glenn and Cappoen, Davie and Vervaet, Chris and Cos, Paul and Coenye, Tom},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {HOST-PATHOGEN INTERACTIONS,CYSTIC-FIBROSIS PATIENTS,CEPACIA COMPLEX,CELL-CULTURE,PSEUDOMONAS-AERUGINOSA,STAPHYLOCOCCUS-AUREUS,ANTIBIOTIC-RESISTANCE,MICROBIAL BIOFILMS,EPITHELIAL-CELLS,MICONAZOLE},
  language     = {eng},
  number       = {3},
  pages        = {16},
  title        = {Evaluation of combination therapy for Burkholderia cenocepacia lung infection in different in vitro and in vivo models},
  url          = {http://dx.doi.org/10.1371/journal.pone.0172723},
  volume       = {12},
  year         = {2017},
}

Chicago
Van den Driessche, Freija, Bieke Vanhoutte, Gilles Brackman, Aurélie Crabbé, Petra Rigole, Jurgen Vercruysse, Glenn Verstraete, et al. 2017. “Evaluation of Combination Therapy for Burkholderia Cenocepacia Lung Infection in Different in Vitro and in Vivo Models.” Plos One 12 (3).
APA
Van den Driessche, Freija, Vanhoutte, B., Brackman, G., Crabbé, A., Rigole, P., Vercruysse, J., Verstraete, G., et al. (2017). Evaluation of combination therapy for Burkholderia cenocepacia lung infection in different in vitro and in vivo models. PLOS ONE, 12(3).
Vancouver
1.
Van den Driessche F, Vanhoutte B, Brackman G, Crabbé A, Rigole P, Vercruysse J, et al. Evaluation of combination therapy for Burkholderia cenocepacia lung infection in different in vitro and in vivo models. PLOS ONE. 2017;12(3).
MLA
Van den Driessche, Freija, Bieke Vanhoutte, Gilles Brackman, et al. “Evaluation of Combination Therapy for Burkholderia Cenocepacia Lung Infection in Different in Vitro and in Vivo Models.” PLOS ONE 12.3 (2017): n. pag. Print.