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Role of phenazines and cyclic lipopeptides produced by pseudomonas sp CMR12a in induced systemic resistance on rice and bean

Zongwang Ma (UGent) , Hoang Gia Khuong Hua (UGent) , Marc Ongena and Monica Höfte (UGent)
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Abstract
Pseudomonas sp. CMR12a produces two different classes of cyclic lipopeptides (CLPs) (orfamides and sessilins), which all play a role in direct antagonism against soilborne pathogens. Here we show that Pseudomonas sp. CMR12a is also able to induce systemic resistance to Magnaporthe oryzae on rice and to the web blight pathogen Rhizoctonia solani AG2-2 on bean. Plant assays with biosynthesis mutants of Pseudomonas sp. CMR12a impaired in the production of phenazines and/or CLPs and purified metabolites revealed that distinct bacterial determinants are responsible for inducing systemic resistance in these two pathosystems. In rice, mutants impaired in phenazine production completely lost their ability to induce systemic resistance, while a soil drench with pure phenazine-1-carboxamide (PCN) at a concentration of 0.1 or 1 mu M was active in inducing resistance against M. oryzae. In bean, mutants that only produced phenazines, sessilins or orfamides were still able to induce systemic resistance against Rhizoctonia web blight, but a balanced production of these metabolites was needed. This study not only shows that Pseudomonas sp. CMR12a can protect rice to blast disease and bean to web blight disease, but also displays that the determinants involved in induced systemic resistance are plant, pathogen and concentration dependent.
Keywords
PATTERN-RECOGNITION RECEPTORS, BIOLOGICAL-CONTROL, BACILLUS-SUBTILIS, FLUORESCENT PSEUDOMONADS, DISEASE RESISTANCE, MAGNAPORTHE-GRISEA, DEFENSE RESPONSES, AERUGINOSA 7NSK2, SALICYLIC-ACID, BLAST FUNGUS

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Chicago
Ma, Zongwang, Hoang Gia Khuong Hua, Marc Ongena, and Monica Höfte. 2016. “Role of Phenazines and Cyclic Lipopeptides Produced by Pseudomonas Sp CMR12a in Induced Systemic Resistance on Rice and Bean.” Environmental Microbiology Reports 8 (5): 896–904.
APA
Ma, Zongwang, Hua, H. G. K., Ongena, M., & Höfte, M. (2016). Role of phenazines and cyclic lipopeptides produced by pseudomonas sp CMR12a in induced systemic resistance on rice and bean. ENVIRONMENTAL MICROBIOLOGY REPORTS, 8(5), 896–904.
Vancouver
1.
Ma Z, Hua HGK, Ongena M, Höfte M. Role of phenazines and cyclic lipopeptides produced by pseudomonas sp CMR12a in induced systemic resistance on rice and bean. ENVIRONMENTAL MICROBIOLOGY REPORTS. 2016;8(5):896–904.
MLA
Ma, Zongwang, Hoang Gia Khuong Hua, Marc Ongena, et al. “Role of Phenazines and Cyclic Lipopeptides Produced by Pseudomonas Sp CMR12a in Induced Systemic Resistance on Rice and Bean.” ENVIRONMENTAL MICROBIOLOGY REPORTS 8.5 (2016): 896–904. Print.
@article{8517597,
  abstract     = {Pseudomonas sp. CMR12a produces two different classes of cyclic lipopeptides (CLPs) (orfamides and sessilins), which all play a role in direct antagonism against soilborne pathogens. Here we show that Pseudomonas sp. CMR12a is also able to induce systemic resistance to Magnaporthe oryzae on rice and to the web blight pathogen Rhizoctonia solani AG2-2 on bean. Plant assays with biosynthesis mutants of Pseudomonas sp. CMR12a impaired in the production of phenazines and/or CLPs and purified metabolites revealed that distinct bacterial determinants are responsible for inducing systemic resistance in these two pathosystems. In rice, mutants impaired in phenazine production completely lost their ability to induce systemic resistance, while a soil drench with pure phenazine-1-carboxamide (PCN) at a concentration of 0.1 or 1 mu M was active in inducing resistance against M. oryzae. In bean, mutants that only produced phenazines, sessilins or orfamides were still able to induce systemic resistance against Rhizoctonia web blight, but a balanced production of these metabolites was needed. This study not only shows that Pseudomonas sp. CMR12a can protect rice to blast disease and bean to web blight disease, but also displays that the determinants involved in induced systemic resistance are plant, pathogen and concentration dependent.},
  author       = {Ma, Zongwang and Hua, Hoang Gia Khuong and Ongena, Marc and H{\"o}fte, Monica},
  issn         = {1758-2229},
  journal      = {ENVIRONMENTAL MICROBIOLOGY REPORTS},
  language     = {eng},
  number       = {5},
  pages        = {896--904},
  title        = {Role of phenazines and cyclic lipopeptides produced by pseudomonas sp CMR12a in induced systemic resistance on rice and bean},
  url          = {http://dx.doi.org/10.1111/1758-2229.12454},
  volume       = {8},
  year         = {2016},
}

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