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Effect of poly-β-hydroxybutyrate on growth, enzyme activity and intestinal microbial community of Chinese mitten crab, Eriocheir sinensis (Milne-Edwards) juveniles

(2016) AQUACULTURE RESEARCH. 47(11). p.3644-3652
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Abstract
Poly-beta-hydroxybutyrate (PHB) is microbial carbon and energy storage polymer, which can be degraded into water-soluble beta-hydroxybutyric acid in the gastrointestinal tract of aquatic animals. A 60-day culture experiment was performed using Chinese mitten crab, Eriocheir sinensis (Milne-Edwards) juveniles with an average initial body weight of 0.74 +/- 0.06 g which were fed a diet supplemented with 0%, 0.5%, 1%, 3% or 5% PHB. A PHB dietary supplementation of 1% and 3% significantly improved the body weight gain, moulting frequency and concomitantly reduced 2nd-3rd moulting intervals of the crabs (P < 0.05). The dietary PHB level positively related to hepatopan-creatic pepsin, trypsin and lipase activity (P < 0.05). Increasing the dietary PHB also improved total superoxide dismutase activity, but reduced alkaline phosphatase and acid phosphatase activity in the serum of hemolymph (P < 0.05). A 16S rRNA gene analysis by denaturing gradient gel electrophoresis indicated that PHB supplementation led to a significantly higher range-weighted richness, diversity and evenness of the gut bacterial community when dosed at 3% in the feed. The beneficial effects of PHB are discussed in terms of immune defense, metabolism and gut microbiota of the crabs.
Keywords
GRADIENT GEL-ELECTROPHORESIS, 16S RIBOSOMAL-RNA, EUROPEAN SEA BASS, GASTROINTESTINAL-TRACT, PERFORMANCE, POPULATIONS, AQUACULTURE, ECOLOGY, LARVAE, PHB, poly-beta-hydroxybutyrate (PHB), Eriocheir sinensis (Milne-Edwards), growth, enzyme activity, gut microbiota

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MLA
Sui, Liying et al. “Effect of Poly-β-hydroxybutyrate on Growth, Enzyme Activity and Intestinal Microbial Community of Chinese Mitten Crab, Eriocheir Sinensis (Milne-Edwards) Juveniles.” AQUACULTURE RESEARCH 47.11 (2016): 3644–3652. Print.
APA
Sui, Liying, Ma, G., Lu, W., Deng, Y., Bossier, P., & De Schryver, P. (2016). Effect of poly-β-hydroxybutyrate on growth, enzyme activity and intestinal microbial community of Chinese mitten crab, Eriocheir sinensis (Milne-Edwards) juveniles. AQUACULTURE RESEARCH, 47(11), 3644–3652.
Chicago author-date
Sui, Liying, Guannan Ma, Wanlong Lu, Yuangao Deng, Peter Bossier, and Peter De Schryver. 2016. “Effect of Poly-β-hydroxybutyrate on Growth, Enzyme Activity and Intestinal Microbial Community of Chinese Mitten Crab, Eriocheir Sinensis (Milne-Edwards) Juveniles.” Aquaculture Research 47 (11): 3644–3652.
Chicago author-date (all authors)
Sui, Liying, Guannan Ma, Wanlong Lu, Yuangao Deng, Peter Bossier, and Peter De Schryver. 2016. “Effect of Poly-β-hydroxybutyrate on Growth, Enzyme Activity and Intestinal Microbial Community of Chinese Mitten Crab, Eriocheir Sinensis (Milne-Edwards) Juveniles.” Aquaculture Research 47 (11): 3644–3652.
Vancouver
1.
Sui L, Ma G, Lu W, Deng Y, Bossier P, De Schryver P. Effect of poly-β-hydroxybutyrate on growth, enzyme activity and intestinal microbial community of Chinese mitten crab, Eriocheir sinensis (Milne-Edwards) juveniles. AQUACULTURE RESEARCH. 2016;47(11):3644–52.
IEEE
[1]
L. Sui, G. Ma, W. Lu, Y. Deng, P. Bossier, and P. De Schryver, “Effect of poly-β-hydroxybutyrate on growth, enzyme activity and intestinal microbial community of Chinese mitten crab, Eriocheir sinensis (Milne-Edwards) juveniles,” AQUACULTURE RESEARCH, vol. 47, no. 11, pp. 3644–3652, 2016.
@article{8517559,
  abstract     = {Poly-beta-hydroxybutyrate (PHB) is microbial carbon and energy storage polymer, which can be degraded into water-soluble beta-hydroxybutyric acid in the gastrointestinal tract of aquatic animals. A 60-day culture experiment was performed using Chinese mitten crab, Eriocheir sinensis (Milne-Edwards) juveniles with an average initial body weight of 0.74 +/- 0.06 g which were fed a diet supplemented with 0%, 0.5%, 1%, 3% or 5% PHB. A PHB dietary supplementation of 1% and 3% significantly improved the body weight gain, moulting frequency and concomitantly reduced 2nd-3rd moulting intervals of the crabs (P < 0.05). The dietary PHB level positively related to hepatopan-creatic pepsin, trypsin and lipase activity (P < 0.05). Increasing the dietary PHB also improved total superoxide dismutase activity, but reduced alkaline phosphatase and acid phosphatase activity in the serum of hemolymph (P < 0.05). A 16S rRNA gene analysis by denaturing gradient gel electrophoresis indicated that PHB supplementation led to a significantly higher range-weighted richness, diversity and evenness of the gut bacterial community when dosed at 3% in the feed. The beneficial effects of PHB are discussed in terms of immune defense, metabolism and gut microbiota of the crabs.},
  author       = {Sui, Liying and Ma, Guannan and Lu, Wanlong and Deng, Yuangao and Bossier, Peter and De Schryver, Peter},
  issn         = {1355-557X},
  journal      = {AQUACULTURE RESEARCH},
  keywords     = {GRADIENT GEL-ELECTROPHORESIS,16S RIBOSOMAL-RNA,EUROPEAN SEA BASS,GASTROINTESTINAL-TRACT,PERFORMANCE,POPULATIONS,AQUACULTURE,ECOLOGY,LARVAE,PHB,poly-beta-hydroxybutyrate (PHB),Eriocheir sinensis (Milne-Edwards),growth,enzyme activity,gut microbiota},
  language     = {eng},
  number       = {11},
  pages        = {3644--3652},
  title        = {Effect of poly-β-hydroxybutyrate on growth, enzyme activity and intestinal microbial community of Chinese mitten crab, Eriocheir sinensis (Milne-Edwards) juveniles},
  url          = {http://dx.doi.org/10.1111/are.12817},
  volume       = {47},
  year         = {2016},
}

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