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The cell density-dependent expression of stewartan exopolysaccharide in Pantoea stewartii ssp. stewartii is a function of EsaR-mediated repression of the rcsA gene

Timothy Minogue, Aurélien Carlier UGent, Maria D Koutsoudis and Susanne B Von Bodman (2005) MOLECULAR MICROBIOLOGY. 56(1). p.189-203
abstract
The LuxR-type quorum-sensing transcription factor EsaR functions as a repressor of exopolysaccharide (EPS) synthesis in the phytopathogenic bacterium Pantoea stewartii ssp. stewartii. The cell density-dependent expression of EPS is critical for Stewart's wilt disease development. Strains deficient in the synthesis of a diffusible acyl-homoserine lactone inducer remain repressed for EPS synthesis and are consequently avirulent. In contrast, disruption of the esaR gene leads to hypermucoidy and attenuated disease development. Ligand-free EsaR functions as a negative autoregulator of the esaR gene and responds to exogenous acyl-homoserine lactone for derepression. The focus of this study was to define the mechanism by which EsaR governs the expression of the cps locus, which encodes functions required for stewartan EPS synthesis and membrane translocation. Genetic and biochemical studies show that EsaR directly represses the transcription of the rcsA gene. RcsA encodes an essential coactivator for RcsA/RcsB-mediated transcriptional activation of cps genes. In vitro assays identify an EsaR DNA binding site within the rcsA promoter that is reasonably well conserved with the previously described esaR box. We also describe that RcsA positively controls its own expression. Interestingly, promoter proximal genes within the cps cluster are significantly more acyl-homoserine lactone responsive than genes located towards the middle or 3' end of the gene cluster. We will discuss a possible role of EsaR-mediated quorum sensing in the differential expression of the cps operon.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ESCHERICHIA-COLI K-12, CAPSULAR POLYSACCHARIDE SYNTHESIS, GRAM-POSITIVE BACTERIA, QUORUM-SENSING SIGNAL, K30 GROUP-1 CAPSULE, LUXR-LUXI FAMILY, ERWINIA-STEWARTII, NEGATIVE BACTERIA, VIBRIO-FISCHERI, OUTER-MEMBRANE
journal title
MOLECULAR MICROBIOLOGY
Mol. Microbiol.
volume
56
issue
1
pages
189 - 203
Web of Science type
Article
Web of Science id
000227499000016
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
6.203 (2005)
JCR rank
33/256 (2005)
JCR quartile
1 (2005)
ISSN
0950-382X
DOI
10.1111/j.1365-2958.2004.04529.x
language
English
UGent publication?
no
classification
A1
id
8517104
handle
http://hdl.handle.net/1854/LU-8517104
date created
2017-04-06 07:37:17
date last changed
2017-08-01 14:17:16
@article{8517104,
  abstract     = {The LuxR-type quorum-sensing transcription factor EsaR functions as a repressor of exopolysaccharide (EPS) synthesis in the phytopathogenic bacterium Pantoea stewartii ssp. stewartii. The cell density-dependent expression of EPS is critical for Stewart's wilt disease development. Strains deficient in the synthesis of a diffusible acyl-homoserine lactone inducer remain repressed for EPS synthesis and are consequently avirulent. In contrast, disruption of the esaR gene leads to hypermucoidy and attenuated disease development. Ligand-free EsaR functions as a negative autoregulator of the esaR gene and responds to exogenous acyl-homoserine lactone for derepression. The focus of this study was to define the mechanism by which EsaR governs the expression of the cps locus, which encodes functions required for stewartan EPS synthesis and membrane translocation. Genetic and biochemical studies show that EsaR directly represses the transcription of the rcsA gene. RcsA encodes an essential coactivator for RcsA/RcsB-mediated transcriptional activation of cps genes. In vitro assays identify an EsaR DNA binding site within the rcsA promoter that is reasonably well conserved with the previously described esaR box. We also describe that RcsA positively controls its own expression. Interestingly, promoter proximal genes within the cps cluster are significantly more acyl-homoserine lactone responsive than genes located towards the middle or 3' end of the gene cluster. We will discuss a possible role of EsaR-mediated quorum sensing in the differential expression of the cps operon.},
  author       = {Minogue, Timothy and Carlier, Aur{\'e}lien and Koutsoudis, Maria D and Von Bodman, Susanne B},
  issn         = {0950-382X},
  journal      = {MOLECULAR MICROBIOLOGY},
  keyword      = {ESCHERICHIA-COLI K-12,CAPSULAR POLYSACCHARIDE SYNTHESIS,GRAM-POSITIVE BACTERIA,QUORUM-SENSING SIGNAL,K30 GROUP-1 CAPSULE,LUXR-LUXI FAMILY,ERWINIA-STEWARTII,NEGATIVE BACTERIA,VIBRIO-FISCHERI,OUTER-MEMBRANE},
  language     = {eng},
  number       = {1},
  pages        = {189--203},
  title        = {The cell density-dependent expression of stewartan exopolysaccharide in Pantoea stewartii ssp. stewartii is a function of EsaR-mediated repression of the rcsA gene},
  url          = {http://dx.doi.org/10.1111/j.1365-2958.2004.04529.x},
  volume       = {56},
  year         = {2005},
}

Chicago
Minogue, Timothy, Aurélien Carlier, Maria D Koutsoudis, and Susanne B Von Bodman. 2005. “The Cell Density-dependent Expression of Stewartan Exopolysaccharide in Pantoea Stewartii Ssp. Stewartii Is a Function of EsaR-mediated Repression of the rcsA Gene.” Molecular Microbiology 56 (1): 189–203.
APA
Minogue, T., Carlier, A., Koutsoudis, M. D., & Von Bodman, S. B. (2005). The cell density-dependent expression of stewartan exopolysaccharide in Pantoea stewartii ssp. stewartii is a function of EsaR-mediated repression of the rcsA gene. MOLECULAR MICROBIOLOGY, 56(1), 189–203.
Vancouver
1.
Minogue T, Carlier A, Koutsoudis MD, Von Bodman SB. The cell density-dependent expression of stewartan exopolysaccharide in Pantoea stewartii ssp. stewartii is a function of EsaR-mediated repression of the rcsA gene. MOLECULAR MICROBIOLOGY. 2005;56(1):189–203.
MLA
Minogue, Timothy, Aurélien Carlier, Maria D Koutsoudis, et al. “The Cell Density-dependent Expression of Stewartan Exopolysaccharide in Pantoea Stewartii Ssp. Stewartii Is a Function of EsaR-mediated Repression of the rcsA Gene.” MOLECULAR MICROBIOLOGY 56.1 (2005): 189–203. Print.