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The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111

Nadine Schmid, Gabriella Pessi, Yinyue Deng, Claudio Aguilar, Aurélien Carlier UGent, Alexander Grunau, Ulrich Omasits, Lian-Hui Zhang, Christian H Ahrens and Leo Eberl (2012) PLOS ONE. 7(11).
abstract
Quorum sensing in Burkholderia cenocepacia H111 involves two signalling systems that depend on different signal molecules, namely N-acyl homoserine lactones (AHLs) and the diffusible signal factor cis-2-dodecenoic acid (BDSF). Previous studies have shown that AHLs and BDSF control similar phenotypic traits, including biofilm formation, proteolytic activity and pathogenicity. In this study we mapped the BDSF stimulon by RNA-Seq and shotgun proteomics analysis. We demonstrate that a set of the identified BDSF-regulated genes or proteins are also controlled by AHLs, suggesting that the two regulons partially overlap. The detailed analysis of two mutually regulated operons, one encoding three lectins and the other one encoding the large surface protein BapA and its type I secretion machinery, revealed that both AHLs and BDSF are required for full expression, suggesting that the two signalling systems operate in parallel. In accordance with this, we show that both AHLs and BDSF are required for biofilm formation and protease production.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CYSTIC-FIBROSIS PATIENTS, SOYBEAN ROOT-NODULES, LUXR-LUXI FAMILY, CEPACIA COMPLEX, TRANSCRIPTIONAL REGULATORS, QUANTITATIVE PROTEOMICS, PSEUDOMONAS-AERUGINOSA, IDENTIFICATION, VIRULENCE, SIGNAL
journal title
PLOS ONE
PLoS One
volume
7
issue
11
article number
e49966
pages
12 pages
Web of Science type
Article
Web of Science id
000311535700059
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.73 (2012)
JCR rank
7/56 (2012)
JCR quartile
1 (2012)
ISSN
1932-6203
DOI
10.1371/journal.pone.0049966
language
English
UGent publication?
no
classification
A1
id
8517097
handle
http://hdl.handle.net/1854/LU-8517097
date created
2017-04-06 07:32:29
date last changed
2017-04-18 09:52:25
@article{8517097,
  abstract     = {Quorum sensing in Burkholderia cenocepacia H111 involves two signalling systems that depend on different signal molecules, namely N-acyl homoserine lactones (AHLs) and the diffusible signal factor cis-2-dodecenoic acid (BDSF). Previous studies have shown that AHLs and BDSF control similar phenotypic traits, including biofilm formation, proteolytic activity and pathogenicity. In this study we mapped the BDSF stimulon by RNA-Seq and shotgun proteomics analysis. We demonstrate that a set of the identified BDSF-regulated genes or proteins are also controlled by AHLs, suggesting that the two regulons partially overlap. The detailed analysis of two mutually regulated operons, one encoding three lectins and the other one encoding the large surface protein BapA and its type I secretion machinery, revealed that both AHLs and BDSF are required for full expression, suggesting that the two signalling systems operate in parallel. In accordance with this, we show that both AHLs and BDSF are required for biofilm formation and protease production.},
  articleno    = {e49966},
  author       = {Schmid, Nadine and Pessi, Gabriella and Deng, Yinyue and Aguilar, Claudio and Carlier, Aur{\'e}lien and Grunau, Alexander and Omasits, Ulrich and Zhang, Lian-Hui and Ahrens, Christian H and Eberl, Leo},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {CYSTIC-FIBROSIS PATIENTS,SOYBEAN ROOT-NODULES,LUXR-LUXI FAMILY,CEPACIA COMPLEX,TRANSCRIPTIONAL REGULATORS,QUANTITATIVE PROTEOMICS,PSEUDOMONAS-AERUGINOSA,IDENTIFICATION,VIRULENCE,SIGNAL},
  language     = {eng},
  number       = {11},
  pages        = {12},
  title        = {The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111},
  url          = {http://dx.doi.org/10.1371/journal.pone.0049966},
  volume       = {7},
  year         = {2012},
}

Chicago
Schmid, Nadine, Gabriella Pessi, Yinyue Deng, Claudio Aguilar, Aurélien Carlier, Alexander Grunau, Ulrich Omasits, Lian-Hui Zhang, Christian H Ahrens, and Leo Eberl. 2012. “The AHL- and BDSF-dependent Quorum Sensing Systems Control Specific and Overlapping Sets of Genes in Burkholderia Cenocepacia H111.” Plos One 7 (11).
APA
Schmid, Nadine, Pessi, G., Deng, Y., Aguilar, C., Carlier, A., Grunau, A., Omasits, U., et al. (2012). The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111. PLOS ONE, 7(11).
Vancouver
1.
Schmid N, Pessi G, Deng Y, Aguilar C, Carlier A, Grunau A, et al. The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111. PLOS ONE. 2012;7(11).
MLA
Schmid, Nadine, Gabriella Pessi, Yinyue Deng, et al. “The AHL- and BDSF-dependent Quorum Sensing Systems Control Specific and Overlapping Sets of Genes in Burkholderia Cenocepacia H111.” PLOS ONE 7.11 (2012): n. pag. Print.