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A novel siderophore-independent strategy of iron uptake in the genus Burkholderia

Anugraha Mathew, Leo Eberl and Aurélien Carlier UGent (2013) MOLECULAR MICROBIOLOGY. 91(4). p.805-820
abstract
Like many other bacteria, Burkholderia sp. take up iron in its ferric form via siderophore-dependent transporters. We observed that mutant strains of B.cenocepaciaH111 unable to synthesize siderophores did not exhibit any growth defect under iron limited conditions. This finding suggested that this opportunistic pathogen can adopt an alternative iron uptake strategy to compensate for the loss of siderophores. We identified a putative iron uptake locus, ftr(Bcc)ABCD, in the genome of B.cenocepaciaH111, which is also conserved in other members of the genus Burkholderia. Mutants deficient in both siderophore-dependent and Ftr(Bcc)ABCD systems failed to grow under iron-limited conditions and radiolabelled iron transport assays showed that these mutants were impaired in iron uptake. In addition, expression of ftr(Bcc)ABCD can restore growth of an E.coli strain lacking all known high-affinity iron transport systems under iron-limited conditions. We show that all four proteins encoded by ftr(Bcc)ABCD are essential for iron uptake. Furthermore, our results indicate that the expression of ftr(Bcc)ABCD is regulated at the transcriptional level by iron concentration. This study provides evidence of an alternative, siderophore-independent, iron uptake system in Burkholderia species.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ESCHERICHIA-COLI K-12, CEPACIA COMPLEX, PSEUDOMONAS-AERUGINOSA, CYSTIC-FIBROSIS, BRUCELLA-ABORTUS, ORNIBACTIN BIOSYNTHESIS, SP-NOV, CENOCEPACIA, TRANSPORTER, VIRULENCE
journal title
MOLECULAR MICROBIOLOGY
Mol. Microbiol.
volume
91
issue
4
pages
805 - 820
Web of Science type
Article
Web of Science id
000331383100015
JCR category
MICROBIOLOGY
JCR impact factor
5.026 (2013)
JCR rank
19/119 (2013)
JCR quartile
1 (2013)
ISSN
0950-382X
DOI
10.1111/mmi.12499
language
English
UGent publication?
no
classification
A1
id
8517093
handle
http://hdl.handle.net/1854/LU-8517093
date created
2017-04-06 07:29:16
date last changed
2017-08-01 14:26:59
@article{8517093,
  abstract     = {Like many other bacteria, Burkholderia sp. take up iron in its ferric form via siderophore-dependent transporters. We observed that mutant strains of B.cenocepaciaH111 unable to synthesize siderophores did not exhibit any growth defect under iron limited conditions. This finding suggested that this opportunistic pathogen can adopt an alternative iron uptake strategy to compensate for the loss of siderophores. We identified a putative iron uptake locus, ftr(Bcc)ABCD, in the genome of B.cenocepaciaH111, which is also conserved in other members of the genus Burkholderia. Mutants deficient in both siderophore-dependent and Ftr(Bcc)ABCD systems failed to grow under iron-limited conditions and radiolabelled iron transport assays showed that these mutants were impaired in iron uptake. In addition, expression of ftr(Bcc)ABCD can restore growth of an E.coli strain lacking all known high-affinity iron transport systems under iron-limited conditions. We show that all four proteins encoded by ftr(Bcc)ABCD are essential for iron uptake. Furthermore, our results indicate that the expression of ftr(Bcc)ABCD is regulated at the transcriptional level by iron concentration. This study provides evidence of an alternative, siderophore-independent, iron uptake system in Burkholderia species.},
  author       = {Mathew, Anugraha and Eberl, Leo and Carlier, Aur{\'e}lien},
  issn         = {0950-382X},
  journal      = {MOLECULAR MICROBIOLOGY},
  keyword      = {ESCHERICHIA-COLI K-12,CEPACIA COMPLEX,PSEUDOMONAS-AERUGINOSA,CYSTIC-FIBROSIS,BRUCELLA-ABORTUS,ORNIBACTIN BIOSYNTHESIS,SP-NOV,CENOCEPACIA,TRANSPORTER,VIRULENCE},
  language     = {eng},
  number       = {4},
  pages        = {805--820},
  title        = {A novel siderophore-independent strategy of iron uptake in the genus Burkholderia},
  url          = {http://dx.doi.org/10.1111/mmi.12499},
  volume       = {91},
  year         = {2013},
}

Chicago
Mathew, Anugraha, Leo Eberl, and Aurélien Carlier. 2013. “A Novel Siderophore-independent Strategy of Iron Uptake in the Genus Burkholderia.” Molecular Microbiology 91 (4): 805–820.
APA
Mathew, A., Eberl, L., & Carlier, A. (2013). A novel siderophore-independent strategy of iron uptake in the genus Burkholderia. MOLECULAR MICROBIOLOGY, 91(4), 805–820.
Vancouver
1.
Mathew A, Eberl L, Carlier A. A novel siderophore-independent strategy of iron uptake in the genus Burkholderia. MOLECULAR MICROBIOLOGY. 2013;91(4):805–20.
MLA
Mathew, Anugraha, Leo Eberl, and Aurélien Carlier. “A Novel Siderophore-independent Strategy of Iron Uptake in the Genus Burkholderia.” MOLECULAR MICROBIOLOGY 91.4 (2013): 805–820. Print.