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The ER stress sensor PERK coordinates ER-plasma membrane contact site formation through interaction with filamin-A and F-actin remodeling

(2017) MOLECULAR CELL. 65(5). p.885-899
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Abstract
Loss of ER Ca2+ homeostasis triggers endoplasmic reticulum (ER) stress and drives ER-PM contact sites formation in order to refill ER-luminal Ca2+. Recent studies suggest that the ER stress sensor and mediator of the unfolded protein response (UPR) PERK regulates intracellular Ca2+ fluxes, but the mechanisms remain elusive. Here, using proximity-dependent biotin identification (BioID), we identified the actin-binding protein Filamin A (FLNA) as a key PERK interactor. Cells lacking PERK accumulate F-actin at the cell edges and display reduced ER-PM contacts. Following ER-Ca2+ store depletion, the PERK-FLNA interaction drives the expansion of ER-PM juxtapositions by regulating F-actin-assisted relocation of the ER-associated tethering proteins Stromal Interaction Molecule 1 (STIM1) and Extended Synaptotagmin-1 (E-Syt1) to the PM. Cytosolic Ca2+ elevation elicits rapid and UPR-independent PERK dimerization, which enforces PERK-FLNA-mediated ER-PM juxtapositions. Collectively, our data unravel an unprecedented role of PERK in the regulation of ER-PM appositions through the modulation of the actin cytoskeleton.
Keywords
UNFOLDED PROTEIN RESPONSE, ENDOPLASMIC-RETICULUM, KINASE, CYTOSKELETON, DIMERIZATION, CALCINEURIN, SECRETION, CELLS, STIM1, STORE

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MLA
van Vliet, Alexander R., et al. “The ER Stress Sensor PERK Coordinates ER-Plasma Membrane Contact Site Formation through Interaction with Filamin-A and F-Actin Remodeling.” MOLECULAR CELL, vol. 65, no. 5, 2017, pp. 885–99, doi:10.1016/j.molcel.2017.01.020.
APA
van Vliet, A. R., Giordano, F., Gerlo, S., Segura, I., Van Eygen, S., Molenberghs, G., … Agostinis, P. (2017). The ER stress sensor PERK coordinates ER-plasma membrane contact site formation through interaction with filamin-A and F-actin remodeling. MOLECULAR CELL, 65(5), 885–899. https://doi.org/10.1016/j.molcel.2017.01.020
Chicago author-date
Vliet, Alexander R van, Francesca Giordano, Sarah Gerlo, Inmaculada Segura, Sofie Van Eygen, Geert Molenberghs, Susana Rocha, et al. 2017. “The ER Stress Sensor PERK Coordinates ER-Plasma Membrane Contact Site Formation through Interaction with Filamin-A and F-Actin Remodeling.” MOLECULAR CELL 65 (5): 885–99. https://doi.org/10.1016/j.molcel.2017.01.020.
Chicago author-date (all authors)
van Vliet, Alexander R, Francesca Giordano, Sarah Gerlo, Inmaculada Segura, Sofie Van Eygen, Geert Molenberghs, Susana Rocha, Audrey Houcine, Rita Derua, Tom Verfaillie, Jeroen Vangindertael, Herlinde De Keersmaecker, Etienne Waelkens, Jan Tavernier, Johan Hofkens, Wim Annaert, Peter Carmeliet, Afshin Samali, Hideaki Mizuno, and Patrizia Agostinis. 2017. “The ER Stress Sensor PERK Coordinates ER-Plasma Membrane Contact Site Formation through Interaction with Filamin-A and F-Actin Remodeling.” MOLECULAR CELL 65 (5): 885–899. doi:10.1016/j.molcel.2017.01.020.
Vancouver
1.
van Vliet AR, Giordano F, Gerlo S, Segura I, Van Eygen S, Molenberghs G, et al. The ER stress sensor PERK coordinates ER-plasma membrane contact site formation through interaction with filamin-A and F-actin remodeling. MOLECULAR CELL. 2017;65(5):885–99.
IEEE
[1]
A. R. van Vliet et al., “The ER stress sensor PERK coordinates ER-plasma membrane contact site formation through interaction with filamin-A and F-actin remodeling,” MOLECULAR CELL, vol. 65, no. 5, pp. 885–899, 2017.
@article{8517041,
  abstract     = {{Loss of ER Ca2+ homeostasis triggers endoplasmic reticulum (ER) stress and drives ER-PM contact sites formation in order to refill ER-luminal Ca2+. Recent studies suggest that the ER stress sensor and mediator of the unfolded protein response (UPR) PERK regulates intracellular Ca2+ fluxes, but the mechanisms remain elusive. Here, using proximity-dependent biotin identification (BioID), we identified the actin-binding protein Filamin A (FLNA) as a key PERK interactor. Cells lacking PERK accumulate F-actin at the cell edges and display reduced ER-PM contacts. Following ER-Ca2+ store depletion, the PERK-FLNA interaction drives the expansion of ER-PM juxtapositions by regulating F-actin-assisted relocation of the ER-associated tethering proteins Stromal Interaction Molecule 1 (STIM1) and Extended Synaptotagmin-1 (E-Syt1) to the PM. Cytosolic Ca2+ elevation elicits rapid and UPR-independent PERK dimerization, which enforces PERK-FLNA-mediated ER-PM juxtapositions. Collectively, our data unravel an unprecedented role of PERK in the regulation of ER-PM appositions through the modulation of the actin cytoskeleton.}},
  author       = {{van Vliet, Alexander R and Giordano, Francesca and Gerlo, Sarah and Segura, Inmaculada and Van Eygen, Sofie and Molenberghs, Geert and Rocha, Susana and Houcine, Audrey and Derua, Rita and Verfaillie, Tom and Vangindertael, Jeroen and De Keersmaecker, Herlinde and Waelkens, Etienne and Tavernier, Jan and Hofkens, Johan and Annaert, Wim and Carmeliet, Peter and Samali, Afshin and Mizuno, Hideaki and Agostinis, Patrizia}},
  issn         = {{1097-2765}},
  journal      = {{MOLECULAR CELL}},
  keywords     = {{UNFOLDED PROTEIN RESPONSE,ENDOPLASMIC-RETICULUM,KINASE,CYTOSKELETON,DIMERIZATION,CALCINEURIN,SECRETION,CELLS,STIM1,STORE}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{885--899}},
  title        = {{The ER stress sensor PERK coordinates ER-plasma membrane contact site formation through interaction with filamin-A and F-actin remodeling}},
  url          = {{http://dx.doi.org/10.1016/j.molcel.2017.01.020}},
  volume       = {{65}},
  year         = {{2017}},
}

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