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cis-Cinnamic acid is a novel, natural auxin efflux inhibitor that promotes lateral root formation

Ward Steenackers, Petr Klíma, Mussa Quareshy, Igor Cesarino, Robert Kumpf, Sander Corneillie UGent, Pedro Araujo, Tom Viaene, Geert Goeminne UGent, Moritz Nowack UGent, et al. (2017) PLANT PHYSIOLOGY. 173(1). p.552-565
abstract
Auxin steers numerous physiological processes in plants, making the tight control of its endogenous levels and spatiotemporal distribution a necessity. This regulation is achieved by different mechanisms, including auxin biosynthesis, metabolic conversions, degradation, and transport. Here, we introduce cis-cinnamic acid (c-CA) as a novel and unique addition to a small group of endogenous molecules affecting in planta auxin concentrations. c-CA is the photo-isomerization product of the phenylpropanoid pathway intermediate trans-CA (t-CA). When grown on c-CA-containing medium, an evolutionary diverse set of plant species were shown to exhibit phenotypes characteristic for high auxin levels, including inhibition of primary root growth, induction of root hairs, and promotion of adventitious and lateral rooting. By molecular docking and receptor binding assays, we showed that c-CA itself is neither an auxin nor an anti-auxin, and auxin profiling data revealed that c-CA does not significantly interfere with auxin biosynthesis. Single cell-based auxin accumulation assays showed that c-CA, and not t-CA, is a potent inhibitor of auxin efflux. Auxin signaling reporters detected changes in spatiotemporal distribution of the auxin response along the root of c-CA-treated plants, and long-distance auxin transport assays showed no inhibition of rootward auxin transport. Overall, these results suggest that the phenotypes of c-CA-treated plants are the consequence of a local change in auxin accumulation, induced by the inhibition of auxin efflux. This work reveals a novel mechanism how plants may regulate auxin levels and adds a novel, naturally occurring molecule to the chemical toolbox for the studies of auxin homeostasis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ARABIDOPSIS-THALIANA, PLANT DEVELOPMENT, CELL-SEPARATION, TRANSPORT, GENE, BIOSYNTHESIS, ACCUMULATION, EXPRESSION, GRADIENTS, RESPONSES
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
173
issue
1
pages
552 - 565
Web of Science type
Article
Web of Science id
000394135800041
ISSN
0032-0889
1532-2548
DOI
10.1104/pp.16.00943
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8516886
handle
http://hdl.handle.net/1854/LU-8516886
date created
2017-04-04 13:09:42
date last changed
2017-07-03 10:03:48
@article{8516886,
  abstract     = {Auxin steers numerous physiological processes in plants, making the tight control of its endogenous levels and spatiotemporal distribution a necessity. This regulation is achieved by different mechanisms, including auxin biosynthesis, metabolic conversions, degradation, and transport. Here, we introduce cis-cinnamic acid (c-CA) as a novel and unique addition to a small group of endogenous molecules affecting in planta auxin concentrations. c-CA is the photo-isomerization product of the phenylpropanoid pathway intermediate trans-CA (t-CA). When grown on c-CA-containing medium, an evolutionary diverse set of plant species were shown to exhibit phenotypes characteristic for high auxin levels, including inhibition of primary root growth, induction of root hairs, and promotion of adventitious and lateral rooting. By molecular docking and receptor binding assays, we showed that c-CA itself is neither an auxin nor an anti-auxin, and auxin profiling data revealed that c-CA does not significantly interfere with auxin biosynthesis. Single cell-based auxin accumulation assays showed that c-CA, and not t-CA, is a potent inhibitor of auxin efflux. Auxin signaling reporters detected changes in spatiotemporal distribution of the auxin response along the root of c-CA-treated plants, and long-distance auxin transport assays showed no inhibition of rootward auxin transport. Overall, these results suggest that the phenotypes of c-CA-treated plants are the consequence of a local change in auxin accumulation, induced by the inhibition of auxin efflux. This work reveals a novel mechanism how plants may regulate auxin levels and adds a novel, naturally occurring molecule to the chemical toolbox for the studies of auxin homeostasis.},
  author       = {Steenackers, Ward and Kl{\'i}ma, Petr and Quareshy, Mussa and Cesarino, Igor and Kumpf, Robert and Corneillie, Sander and Araujo, Pedro and Viaene, Tom and Goeminne, Geert and Nowack, Moritz and Ljung, Karin and Friml, Ji\v{r}{\'i} and Blakeslee, Joshua J and Nov{\'a}k, Ondrej and Za\v{z}{\'i}malov{\'a}, Eva and Napier, Richard and Boerjan, Wout and Vanholme, Bartel},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {ARABIDOPSIS-THALIANA,PLANT DEVELOPMENT,CELL-SEPARATION,TRANSPORT,GENE,BIOSYNTHESIS,ACCUMULATION,EXPRESSION,GRADIENTS,RESPONSES},
  language     = {eng},
  number       = {1},
  pages        = {552--565},
  title        = {cis-Cinnamic acid is a novel, natural auxin efflux inhibitor that promotes lateral root formation},
  url          = {http://dx.doi.org/10.1104/pp.16.00943},
  volume       = {173},
  year         = {2017},
}

Chicago
Steenackers, Ward, Petr Klíma, Mussa Quareshy, Igor Cesarino, Robert Kumpf, Sander Corneillie, Pedro Araujo, et al. 2017. “cis-Cinnamic Acid Is a Novel, Natural Auxin Efflux Inhibitor That Promotes Lateral Root Formation.” Plant Physiology 173 (1): 552–565.
APA
Steenackers, W., Klíma, P., Quareshy, M., Cesarino, I., Kumpf, R., Corneillie, S., Araujo, P., et al. (2017). cis-Cinnamic acid is a novel, natural auxin efflux inhibitor that promotes lateral root formation. PLANT PHYSIOLOGY, 173(1), 552–565.
Vancouver
1.
Steenackers W, Klíma P, Quareshy M, Cesarino I, Kumpf R, Corneillie S, et al. cis-Cinnamic acid is a novel, natural auxin efflux inhibitor that promotes lateral root formation. PLANT PHYSIOLOGY. 2017;173(1):552–65.
MLA
Steenackers, Ward, Petr Klíma, Mussa Quareshy, et al. “cis-Cinnamic Acid Is a Novel, Natural Auxin Efflux Inhibitor That Promotes Lateral Root Formation.” PLANT PHYSIOLOGY 173.1 (2017): 552–565. Print.