Quantitative microscopic analysis of plasma membrane receptor dynamics in living plant cells
- Author
- Yu Luo (UGent) and Eugenia Russinova (UGent)
- Organization
- Abstract
- Plasma membrane-localized receptors are essential for cellular communication and signal transduction. In Arabidopsis thaliana, BRASSINOSTEROID INSENSITIVE1 (BRI1) is one of the receptors that is activated by binding to its ligand, the brassinosteroid (BR) hormone, at the cell surface to regulate diverse plant developmental processes. The availability of BRI1 in the plasma membrane is related to its signaling output and is known to be controlled by the dynamic endomembrane trafficking. Advances in fluorescence labeling and confocal microscopy techniques enabled us to gain a better understanding of plasma membrane receptor dynamics in living cells. Here we describe different quantitative microscopy methods to monitor the relative steady-state levels of the BRI1 protein in the plasma membrane of root epidermal cells and its relative exocytosis and recycling rates. The methods can be applied also to analyze similar dynamics of other plasma membrane-localized receptors.
- Keywords
- BRI1, Brassinosteroids, Confocal microscopy, Exocytosis, FRAP, Fluorescence, Plasma membrane, Recycling
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8516867
- MLA
- Luo, Yu, and Eugenia Russinova. “Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells.” Brassinosteroids : Methods and Protocols, edited by Eugenia Russinova and Ana I Caño-Delgado, vol. 1564, Springer Humana Press, 2017, pp. 121–32, doi:10.1007/978-1-4939-6813-8_10.
- APA
- Luo, Y., & Russinova, E. (2017). Quantitative microscopic analysis of plasma membrane receptor dynamics in living plant cells. In E. Russinova & A. I. Caño-Delgado (Eds.), Brassinosteroids : methods and protocols (Vol. 1564, pp. 121–132). https://doi.org/10.1007/978-1-4939-6813-8_10
- Chicago author-date
- Luo, Yu, and Eugenia Russinova. 2017. “Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells.” In Brassinosteroids : Methods and Protocols, edited by Eugenia Russinova and Ana I Caño-Delgado, 1564:121–32. New York, NY, USA: Springer Humana Press. https://doi.org/10.1007/978-1-4939-6813-8_10.
- Chicago author-date (all authors)
- Luo, Yu, and Eugenia Russinova. 2017. “Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells.” In Brassinosteroids : Methods and Protocols, ed by. Eugenia Russinova and Ana I Caño-Delgado, 1564:121–132. New York, NY, USA: Springer Humana Press. doi:10.1007/978-1-4939-6813-8_10.
- Vancouver
- 1.Luo Y, Russinova E. Quantitative microscopic analysis of plasma membrane receptor dynamics in living plant cells. In: Russinova E, Caño-Delgado AI, editors. Brassinosteroids : methods and protocols. New York, NY, USA: Springer Humana Press; 2017. p. 121–32.
- IEEE
- [1]Y. Luo and E. Russinova, “Quantitative microscopic analysis of plasma membrane receptor dynamics in living plant cells,” in Brassinosteroids : methods and protocols, vol. 1564, E. Russinova and A. I. Caño-Delgado, Eds. New York, NY, USA: Springer Humana Press, 2017, pp. 121–132.
@incollection{8516867,
abstract = {{Plasma membrane-localized receptors are essential for cellular communication and signal transduction. In Arabidopsis thaliana, BRASSINOSTEROID INSENSITIVE1 (BRI1) is one of the receptors that is activated by binding to its ligand, the brassinosteroid (BR) hormone, at the cell surface to regulate diverse plant developmental processes. The availability of BRI1 in the plasma membrane is related to its signaling output and is known to be controlled by the dynamic endomembrane trafficking. Advances in fluorescence labeling and confocal microscopy techniques enabled us to gain a better understanding of plasma membrane receptor dynamics in living cells. Here we describe different quantitative microscopy methods to monitor the relative steady-state levels of the BRI1 protein in the plasma membrane of root epidermal cells and its relative exocytosis and recycling rates. The methods can be applied also to analyze similar dynamics of other plasma membrane-localized receptors.}},
author = {{Luo, Yu and Russinova, Eugenia}},
booktitle = {{Brassinosteroids : methods and protocols}},
editor = {{Russinova, Eugenia and Caño-Delgado, Ana I}},
isbn = {{9781493968114}},
issn = {{1064-3745}},
keywords = {{BRI1,Brassinosteroids,Confocal microscopy,Exocytosis,FRAP,Fluorescence,Plasma membrane,Recycling}},
language = {{eng}},
pages = {{121--132}},
publisher = {{Springer Humana Press}},
series = {{Methods in Molecular Biology}},
title = {{Quantitative microscopic analysis of plasma membrane receptor dynamics in living plant cells}},
url = {{http://doi.org/10.1007/978-1-4939-6813-8_10}},
volume = {{1564}},
year = {{2017}},
}
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