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Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM

Mathieu Ingouff, Benjamin Selles, Caroline Michaud, Thiet M Vu, Frédéric Berger, Andrea J Schorn, Daphné Autran, Matthias Van Durme, Moritz Nowack UGent, Robert A Martienssen, et al. (2017) GENES & DEVELOPMENT. 31(1). p.72-83
abstract
Cytosine methylation is a key epigenetic mark in many organisms, important for both transcriptional control and genome integrity. While relatively stable during somatic growth, DNA methylation is reprogrammed genome-wide during mammalian reproduction. Reprogramming is essential for zygotic totipotency and to prevent transgenerational inheritance of epimutations. However, the extent of DNA methylation reprogramming in plants remains unclear. Here, we developed sensors reporting with single-cell resolution CG and non-CG methylation in Arabidopsis. Live imaging during reproduction revealed distinct and sex-specific dynamics for both contexts. We found that CHH methylation in the egg cell depends on DOMAINS REARRANGED METHYLASE 2 (DRM2) and RNA polymerase V (Pol V), two main actors of RNA-directed DNA methylation, but does not depend on Pol IV. Our sensors provide insight into global DNA methylation dynamics at the single-cell level with high temporal resolution and offer a powerful tool to track CG and non-CG methylation both during development and in response to environmental cues in all organisms with methylated DNA, as we illustrate in mouse embryonic stem cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
EPIGENETIC INHERITANCE, EARLY EMBRYOGENESIS, SMALL RNA, PLANT, THALIANA, GENOME, CHROMATIN, HETEROCHROMATIN, DEMETHYLATION, ENDOSPERM, DNA methylation, sensors, reprogramming, reproduction
journal title
GENES & DEVELOPMENT
Genes Dev.
volume
31
issue
1
pages
72 - 83
Web of Science type
Article
Web of Science id
000393726000009
ISSN
0890-9369
1549-5477
DOI
10.1101/gad.289397.116
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8516864
handle
http://hdl.handle.net/1854/LU-8516864
date created
2017-04-04 12:26:33
date last changed
2017-08-01 09:29:33
@article{8516864,
  abstract     = {Cytosine methylation is a key epigenetic mark in many organisms, important for both transcriptional control and genome integrity. While relatively stable during somatic growth, DNA methylation is reprogrammed genome-wide during mammalian reproduction. Reprogramming is essential for zygotic totipotency and to prevent transgenerational inheritance of epimutations. However, the extent of DNA methylation reprogramming in plants remains unclear. Here, we developed sensors reporting with single-cell resolution CG and non-CG methylation in Arabidopsis. Live imaging during reproduction revealed distinct and sex-specific dynamics for both contexts. We found that CHH methylation in the egg cell depends on DOMAINS REARRANGED METHYLASE 2 (DRM2) and RNA polymerase V (Pol V), two main actors of RNA-directed DNA methylation, but does not depend on Pol IV. Our sensors provide insight into global DNA methylation dynamics at the single-cell level with high temporal resolution and offer a powerful tool to track CG and non-CG methylation both during development and in response to environmental cues in all organisms with methylated DNA, as we illustrate in mouse embryonic stem cells.},
  author       = {Ingouff, Mathieu and Selles, Benjamin and Michaud, Caroline and Vu, Thiet M and Berger, Fr{\'e}d{\'e}ric and Schorn, Andrea J and Autran, Daphn{\'e} and Van Durme, Matthias and Nowack, Moritz and Martienssen, Robert A and Grimanelli, Daniel},
  issn         = {0890-9369},
  journal      = {GENES \& DEVELOPMENT},
  keyword      = {EPIGENETIC INHERITANCE,EARLY EMBRYOGENESIS,SMALL RNA,PLANT,THALIANA,GENOME,CHROMATIN,HETEROCHROMATIN,DEMETHYLATION,ENDOSPERM,DNA methylation,sensors,reprogramming,reproduction},
  language     = {eng},
  number       = {1},
  pages        = {72--83},
  title        = {Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM},
  url          = {http://dx.doi.org/10.1101/gad.289397.116},
  volume       = {31},
  year         = {2017},
}

Chicago
Ingouff, Mathieu, Benjamin Selles, Caroline Michaud, Thiet M Vu, Frédéric Berger, Andrea J Schorn, Daphné Autran, et al. 2017. “Live-cell Analysis of DNA Methylation During Sexual Reproduction in Arabidopsis Reveals Context and Sex-specific Dynamics Controlled by Noncanonical RdDM.” Genes & Development 31 (1): 72–83.
APA
Ingouff, M., Selles, B., Michaud, C., Vu, T. M., Berger, F., Schorn, A. J., Autran, D., et al. (2017). Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM. GENES & DEVELOPMENT, 31(1), 72–83.
Vancouver
1.
Ingouff M, Selles B, Michaud C, Vu TM, Berger F, Schorn AJ, et al. Live-cell analysis of DNA methylation during sexual reproduction in Arabidopsis reveals context and sex-specific dynamics controlled by noncanonical RdDM. GENES & DEVELOPMENT. 2017;31(1):72–83.
MLA
Ingouff, Mathieu, Benjamin Selles, Caroline Michaud, et al. “Live-cell Analysis of DNA Methylation During Sexual Reproduction in Arabidopsis Reveals Context and Sex-specific Dynamics Controlled by Noncanonical RdDM.” GENES & DEVELOPMENT 31.1 (2017): 72–83. Print.