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Ubiquitylation activates a peptidase that promotes cleavage and destabilization of its activating E3 ligases and diverse growth regulatory proteins to limit cell proliferation in Arabidopsis

(2017) GENES & DEVELOPMENT. 31(2). p.197-208
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
The characteristic shapes and sizes of organs are established by cell proliferation patterns and final cell sizes, but the underlying molecular mechanisms coordinating these are poorly understood. Here we characterize a ubiquitin-activated peptidase called DA1 that limits the duration of cell proliferation during organ growth in Arabidopsis thaliana. The peptidase is activated by two RING E3 ligases, Big Brother (BB) and DA2, which are subsequently cleaved by the activated peptidase and destabilized. In the case of BB, cleavage leads to destabilization by the RING E3 ligase PROTEOLYSIS 1 (PRT1) of the N-end rule pathway. DA1 peptidase activity also cleaves the deubiquitylase UBP15, which promotes cell proliferation, and the transcription factors TEOSINTE BRANCED 1/ CYCLOIDEA/PCF 15 (TCP15) and TCP22, which promote cell proliferation and repress endoreduplication. We propose that DA1 peptidase activity regulates the duration of cell proliferation and the transition to endoreduplication and differentiation during organ formation in plants by coordinating the destabilization of regulatory proteins.
Keywords
ubiquitylation, organ size, Arabidopsis, ubiquitin-activated peptidase, N-end rule-mediated degradation, END RULE PATHWAY, UBIQUITIN-BINDING PROTEINS, ORGAN SIZE, COUPLED MONOUBIQUITINATION, GENE FAMILY, LEAF DEVELOPMENT, BIG BROTHER, THALIANA, DA1, CYCLE

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Citation

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Chicago
Dong, Hui, Jack Dumenil, Fu-Hao Lu, Li Na, Hannes Vanhaeren, Christin Naumann, Maria Klecker, et al. 2017. “Ubiquitylation Activates a Peptidase That Promotes Cleavage and Destabilization of Its Activating E3 Ligases and Diverse Growth Regulatory Proteins to Limit Cell Proliferation in Arabidopsis.” Genes & Development 31 (2): 197–208.
APA
Dong, H., Dumenil, J., Lu, F.-H., Na, L., Vanhaeren, H., Naumann, C., Klecker, M., et al. (2017). Ubiquitylation activates a peptidase that promotes cleavage and destabilization of its activating E3 ligases and diverse growth regulatory proteins to limit cell proliferation in Arabidopsis. GENES & DEVELOPMENT, 31(2), 197–208.
Vancouver
1.
Dong H, Dumenil J, Lu F-H, Na L, Vanhaeren H, Naumann C, et al. Ubiquitylation activates a peptidase that promotes cleavage and destabilization of its activating E3 ligases and diverse growth regulatory proteins to limit cell proliferation in Arabidopsis. GENES & DEVELOPMENT. 2017;31(2):197–208.
MLA
Dong, Hui, Jack Dumenil, Fu-Hao Lu, et al. “Ubiquitylation Activates a Peptidase That Promotes Cleavage and Destabilization of Its Activating E3 Ligases and Diverse Growth Regulatory Proteins to Limit Cell Proliferation in Arabidopsis.” GENES & DEVELOPMENT 31.2 (2017): 197–208. Print.
@article{8516857,
  abstract     = {The characteristic shapes and sizes of organs are established by cell proliferation patterns and final cell sizes, but the underlying molecular mechanisms coordinating these are poorly understood. Here we characterize a ubiquitin-activated peptidase called DA1 that limits the duration of cell proliferation during organ growth in Arabidopsis thaliana. The peptidase is activated by two RING E3 ligases, Big Brother (BB) and DA2, which are subsequently cleaved by the activated peptidase and destabilized. In the case of BB, cleavage leads to destabilization by the RING E3 ligase PROTEOLYSIS 1 (PRT1) of the N-end rule pathway. DA1 peptidase activity also cleaves the deubiquitylase UBP15, which promotes cell proliferation, and the transcription factors TEOSINTE BRANCED 1/ CYCLOIDEA/PCF 15 (TCP15) and TCP22, which promote cell proliferation and repress endoreduplication. We propose that DA1 peptidase activity regulates the duration of cell proliferation and the transition to endoreduplication and differentiation during organ formation in plants by coordinating the destabilization of regulatory proteins.},
  author       = {Dong, Hui and Dumenil, Jack and Lu, Fu-Hao and Na, Li and Vanhaeren, Hannes and Naumann, Christin and Klecker, Maria and Prior, Rachel and Smith, Caroline and McKenzie, Neil and Saalbach, Gerhard and Chen, Liangliang and Xia, Tian and Gonzalez Sanchez, Nathalie and Seguela, Mathilde and Inz{\'e}, Dirk and Dissmeyer, Nico and Li, Yunhai and Bevan, Michael W},
  issn         = {0890-9369},
  journal      = {GENES \& DEVELOPMENT},
  keyword      = {ubiquitylation,organ size,Arabidopsis,ubiquitin-activated peptidase,N-end rule-mediated degradation,END RULE PATHWAY,UBIQUITIN-BINDING PROTEINS,ORGAN SIZE,COUPLED MONOUBIQUITINATION,GENE FAMILY,LEAF DEVELOPMENT,BIG BROTHER,THALIANA,DA1,CYCLE},
  language     = {eng},
  number       = {2},
  pages        = {197--208},
  title        = {Ubiquitylation activates a peptidase that promotes cleavage and destabilization of its activating E3 ligases and diverse growth regulatory proteins to limit cell proliferation in Arabidopsis},
  url          = {http://dx.doi.org/10.1101/gad.292235.116},
  volume       = {31},
  year         = {2017},
}

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