Advanced search
1 file | 535.81 KB Add to list
Author
Organization
Abstract
Three isolates (A19(T), C21 and F12) with spiral-shaped cells and one bipolar sheathed flagellum were obtained from gastric mucosa and caecal contents of three different wild boars (Sus scrofa) and subjected to a polyphasic taxonomic study. A genus-specific PCR showed that these isolates belonged to the genus Helicobacter. Phylogenetic analysis based on 16S rRNA, 60-kDa heat-shock protein (HSP60) and atpA genes demonstrated they formed a novel lineage within this genus. Pairwise 16S rRNA, HSP60 and atpA gene sequence comparisons of the three isolates revealed 99.7, 99.4 and 99.9% similarity, respectively, among the three isolates; the 16S rRNA gene of isolate A19(T) shared 98.5% sequence similarity with its nearest validly named neighbouring species, Helicobacter mastomyrinus (to the type strain MIT 97-5577(T)). The taxonomic uniqueness of the wild boar isolates was confirmed by protein analysis performed by matrix-assisted laser desorption/ionization time-of-flight MS and by a distinctive biochemical profile. These data indicated that isolates A19(T), C21 and F12 represent a novel taxon, for which the name Helicobacter apri sp. nov. is proposed, with isolate A19(T) (= DSM 28990(T) = LMG 28471(T)) as the type strain.
Keywords
GEN-NOV, CAMPYLOBACTER, FECES, BIRD

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 535.81 KB

Citation

Please use this url to cite or link to this publication:

MLA
Zanoni, Renato Giulio, et al. “Helicobacter Apri Sp. Nov., Isolated from Wild Boars.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 66, no. 8, 2016, pp. 2876–82, doi:10.1099/ijsem.0.001071.
APA
Zanoni, R. G., Piva, S., Florio, D., Bassi, P., Mion, D., Cnockaert, M., … Vandamme, P. (2016). Helicobacter apri sp. nov., isolated from wild boars. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 66(8), 2876–2882. https://doi.org/10.1099/ijsem.0.001071
Chicago author-date
Zanoni, Renato Giulio, Silvia Piva, Daniela Florio, Patrizia Bassi, Domenico Mion, Margo Cnockaert, Andrea Luchetti, and Peter Vandamme. 2016. “Helicobacter Apri Sp. Nov., Isolated from Wild Boars.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 66 (8): 2876–82. https://doi.org/10.1099/ijsem.0.001071.
Chicago author-date (all authors)
Zanoni, Renato Giulio, Silvia Piva, Daniela Florio, Patrizia Bassi, Domenico Mion, Margo Cnockaert, Andrea Luchetti, and Peter Vandamme. 2016. “Helicobacter Apri Sp. Nov., Isolated from Wild Boars.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 66 (8): 2876–2882. doi:10.1099/ijsem.0.001071.
Vancouver
1.
Zanoni RG, Piva S, Florio D, Bassi P, Mion D, Cnockaert M, et al. Helicobacter apri sp. nov., isolated from wild boars. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2016;66(8):2876–82.
IEEE
[1]
R. G. Zanoni et al., “Helicobacter apri sp. nov., isolated from wild boars,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 66, no. 8, pp. 2876–2882, 2016.
@article{8515578,
  abstract     = {{Three isolates (A19(T), C21 and F12) with spiral-shaped cells and one bipolar sheathed flagellum were obtained from gastric mucosa and caecal contents of three different wild boars (Sus scrofa) and subjected to a polyphasic taxonomic study. A genus-specific PCR showed that these isolates belonged to the genus Helicobacter. Phylogenetic analysis based on 16S rRNA, 60-kDa heat-shock protein (HSP60) and atpA genes demonstrated they formed a novel lineage within this genus. Pairwise 16S rRNA, HSP60 and atpA gene sequence comparisons of the three isolates revealed 99.7, 99.4 and 99.9% similarity, respectively, among the three isolates; the 16S rRNA gene of isolate A19(T) shared 98.5% sequence similarity with its nearest validly named neighbouring species, Helicobacter mastomyrinus (to the type strain MIT 97-5577(T)). The taxonomic uniqueness of the wild boar isolates was confirmed by protein analysis performed by matrix-assisted laser desorption/ionization time-of-flight MS and by a distinctive biochemical profile. These data indicated that isolates A19(T), C21 and F12 represent a novel taxon, for which the name Helicobacter apri sp. nov. is proposed, with isolate A19(T) (= DSM 28990(T) = LMG 28471(T)) as the type strain.}},
  author       = {{Zanoni, Renato Giulio and Piva, Silvia and Florio, Daniela and Bassi, Patrizia and Mion, Domenico and Cnockaert, Margo and Luchetti, Andrea and Vandamme, Peter}},
  issn         = {{1466-5026}},
  journal      = {{INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY}},
  keywords     = {{GEN-NOV,CAMPYLOBACTER,FECES,BIRD}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{2876--2882}},
  title        = {{Helicobacter apri sp. nov., isolated from wild boars}},
  url          = {{http://doi.org/10.1099/ijsem.0.001071}},
  volume       = {{66}},
  year         = {{2016}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: