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Modulation of the allergen-induced human IgE response in Hu-SCID mice : inhibitory effect of human recombinant IFN-gamma and allergen-derived lipopeptide

(2001) EUROPEAN CYTOKINE NETWORK. 12(3). p.453-461
Author
Organization
Abstract
We have previously established a model to study the in vivo human IgE response using humanized SCID mice. Allergic SCID mice were obtained following intraperitonal injection with mononuclear cells from Dermatophagoides pteronyssinus (Dpt)-sensitive patients, and sensitization by Dpt allergen intraperitonal injection (immunization) or Dpt aerosol (inhalation). Human serum IgE was measured in allergic SCID mice after administration of human recombinant IFN-gamma or the lipopeptide LP 52-71 (derived from peptide p52-71 from Der p 1, Dpt major allergen, coupled to a lipophilic moiety), during the immunization or the inhalation phase. IFN-gamma inhibited human IgE production when given at the time of immunization, but not during inhalation. This effect was long-lasting as Dpt aerosol, given one month after immunization and IFN-gamma administration, failed to increase IgE levels. Unlike Dpt or p52-71, LP 52-71 failed to induce human IgE production at day 14 and 21 after its injection, but did inhibit the development of the IgE response after a secondary Dpt-challenge. Moreover, LP 52-71 administration 14 days after Dpt inhalation decreased IgE levels, in contrast to peptide 52-71, which increased IgE levels. Thus, taken together these results indicate that the development of the human IgE response in allergic SCID mice can be modulated by modified allergen and a Th1 cytokine.
Keywords
SCID mouse, IgE, IFN-gamma, lipopeptide, house dust mite, DER-P-I, IMMUNOGLOBULIN-E PRODUCTION, DUST MITE ALLERGEN, IMMUNE-RESPONSE, T-CELLS, INTERFERON-GAMMA, DERMATOPHAGOIDES-PTERONYSSINUS, AIRWAYS RESPONSIVENESS, LYMPHOCYTE-RESPONSES, ANTIGEN

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Chicago
Duez, Catherine, Helene Gras-Masse, Hamida Hammad, Hikmat Akoum, Alain Didierlaurant, Claude Andre, Andre-Bernard Tonnel, and Joel Pestel. 2001. “Modulation of the Allergen-induced Human IgE Response in Hu-SCID Mice : Inhibitory Effect of Human Recombinant IFN-gamma and Allergen-derived Lipopeptide.” European Cytokine Network 12 (3): 453–461.
APA
Duez, C., Gras-Masse, H., Hammad, H., Akoum, H., Didierlaurant, A., Andre, C., Tonnel, A.-B., et al. (2001). Modulation of the allergen-induced human IgE response in Hu-SCID mice : inhibitory effect of human recombinant IFN-gamma and allergen-derived lipopeptide. EUROPEAN CYTOKINE NETWORK, 12(3), 453–461.
Vancouver
1.
Duez C, Gras-Masse H, Hammad H, Akoum H, Didierlaurant A, Andre C, et al. Modulation of the allergen-induced human IgE response in Hu-SCID mice : inhibitory effect of human recombinant IFN-gamma and allergen-derived lipopeptide. EUROPEAN CYTOKINE NETWORK. 2001;12(3):453–61.
MLA
Duez, Catherine et al. “Modulation of the Allergen-induced Human IgE Response in Hu-SCID Mice : Inhibitory Effect of Human Recombinant IFN-gamma and Allergen-derived Lipopeptide.” EUROPEAN CYTOKINE NETWORK 12.3 (2001): 453–461. Print.
@article{8515505,
  abstract     = {We have previously established a model to study the in vivo human IgE response using humanized SCID mice. Allergic SCID mice were obtained following intraperitonal injection with mononuclear cells from Dermatophagoides pteronyssinus (Dpt)-sensitive patients, and sensitization by Dpt allergen intraperitonal injection (immunization) or Dpt aerosol (inhalation). Human serum IgE was measured in allergic SCID mice after administration of human recombinant IFN-gamma or the lipopeptide LP 52-71 (derived from peptide p52-71 from Der p 1, Dpt major allergen, coupled to a lipophilic moiety), during the immunization or the inhalation phase. IFN-gamma inhibited human IgE production when given at the time of immunization, but not during inhalation. This effect was long-lasting as Dpt aerosol, given one month after immunization and IFN-gamma administration, failed to increase IgE levels. Unlike Dpt or p52-71, LP 52-71 failed to induce human IgE production at day 14 and 21 after its injection, but did inhibit the development of the IgE response after a secondary Dpt-challenge. Moreover, LP 52-71 administration 14 days after Dpt inhalation decreased IgE levels, in contrast to peptide 52-71, which increased IgE levels. Thus, taken together these results indicate that the development of the human IgE response in allergic SCID mice can be modulated by modified allergen and a Th1 cytokine.},
  author       = {Duez, Catherine and Gras-Masse, Helene and Hammad, Hamida and Akoum, Hikmat and Didierlaurant, Alain and Andre, Claude and Tonnel, Andre-Bernard and Pestel, Joel},
  issn         = {1148-5493},
  journal      = {EUROPEAN CYTOKINE NETWORK},
  keywords     = {SCID mouse,IgE,IFN-gamma,lipopeptide,house dust mite,DER-P-I,IMMUNOGLOBULIN-E PRODUCTION,DUST MITE ALLERGEN,IMMUNE-RESPONSE,T-CELLS,INTERFERON-GAMMA,DERMATOPHAGOIDES-PTERONYSSINUS,AIRWAYS RESPONSIVENESS,LYMPHOCYTE-RESPONSES,ANTIGEN},
  language     = {eng},
  number       = {3},
  pages        = {453--461},
  title        = {Modulation of the allergen-induced human IgE response in Hu-SCID mice : inhibitory effect of human recombinant IFN-gamma and allergen-derived lipopeptide},
  volume       = {12},
  year         = {2001},
}

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