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Three-dimensional reconstruction of the tissue-specific multielemental distribution within Ceriodaphnia dubia via multimodal registration using laser ablation ICP-mass spectrometry and X-ray spectroscopic techniques

Stijn Van Malderen (UGent) , Brecht Laforce (UGent) , Thibaut Van Acker (UGent) , Charlotte Nys (UGent) , Maarten De Rijcke (UGent) , Riet De Rycke (UGent) , Michiel De Bruyne (UGent) , Matthieu Boone (UGent) , Karel De Schamphelaere (UGent) , Olga Borovinskaya, et al.
(2017) ANALYTICAL CHEMISTRY. 89(7). p.4161-4168
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Abstract
In this work, the three-dimensional elemental, distribution profile within the freshwater crustacean Ceriodaphnia dubia was constructed at a spatial resolution down to S mu m via a data, fusion approach employing state-of-the-art laser ablation inductively coupled plasma-time-of-flight mass spectrometry (LAICP-TOFMS) and laboratory-based absorption microcomputed tomography (mu-CT). C. dubia was exposed to elevated Cu, Ni, and Zn concentrations, chemically fixed, dehydrated, stained, and embedded, prior to mu-CT analysis. Subsequently, the sample was cut into 5 pm thin sections that were subjected to LA-ICPTOFMS imaging. Multimodal image registration was performed to spatially align the 2D LA-ICP-TOFMS images relative to the Corresponding slices of the 3D mu-CT reconstruction. Mass channels corresponding to the isotopes of a single element were merged to improve the signal-to-noise ratios within the elemental images. In order to aid the visual interpretation of the data, LA-ICP-TOEMS data wete projected onto the mu-CT voxels representing tissue. Additionally, the image resolution and elemental sensitivity were compared to those obtained with synchrotron radiation based 3D confocal mu-X-ray fluorescence imaging upon a chemically fixed and air-dried C. dubia specimen.
Keywords
DAPHNIA-MAGNA, GEOLOGICAL SAMPLES, MIXTURE TOXICITY, HIGH-RESOLUTION, HIGH-SPEED, ZINC, NICKEL, ZN, INSTRUMENTATION, ASSIMILATION

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Chicago
Van Malderen, Stijn, Brecht Laforce, Thibaut Van Acker, Charlotte Nys, Maarten De Rijcke, Riet De Rycke, Michiel De Bruyne, et al. 2017. “Three-dimensional Reconstruction of the Tissue-specific Multielemental Distribution Within Ceriodaphnia Dubia via Multimodal Registration Using Laser Ablation ICP-mass Spectrometry and X-ray Spectroscopic Techniques.” Analytical Chemistry 89 (7): 4161–4168.
APA
Van Malderen, Stijn, Laforce, B., Van Acker, T., Nys, C., De Rijcke, M., De Rycke, R., De Bruyne, M., et al. (2017). Three-dimensional reconstruction of the tissue-specific multielemental distribution within Ceriodaphnia dubia via multimodal registration using laser ablation ICP-mass spectrometry and X-ray spectroscopic techniques. ANALYTICAL CHEMISTRY, 89(7), 4161–4168.
Vancouver
1.
Van Malderen S, Laforce B, Van Acker T, Nys C, De Rijcke M, De Rycke R, et al. Three-dimensional reconstruction of the tissue-specific multielemental distribution within Ceriodaphnia dubia via multimodal registration using laser ablation ICP-mass spectrometry and X-ray spectroscopic techniques. ANALYTICAL CHEMISTRY. 2017;89(7):4161–8.
MLA
Van Malderen, Stijn, Brecht Laforce, Thibaut Van Acker, et al. “Three-dimensional Reconstruction of the Tissue-specific Multielemental Distribution Within Ceriodaphnia Dubia via Multimodal Registration Using Laser Ablation ICP-mass Spectrometry and X-ray Spectroscopic Techniques.” ANALYTICAL CHEMISTRY 89.7 (2017): 4161–4168. Print.
@article{8515288,
  abstract     = {In this work, the three-dimensional elemental, distribution profile within the freshwater crustacean Ceriodaphnia dubia was constructed at a spatial resolution down to S mu m via a data, fusion approach employing state-of-the-art laser ablation inductively coupled plasma-time-of-flight mass spectrometry (LAICP-TOFMS) and laboratory-based absorption microcomputed tomography (mu-CT). C. dubia was exposed to elevated Cu, Ni, and Zn concentrations, chemically fixed, dehydrated, stained, and embedded, prior to mu-CT analysis. Subsequently, the sample was cut into 5 pm thin sections that were subjected to LA-ICPTOFMS imaging. Multimodal image registration was performed to spatially align the 2D LA-ICP-TOFMS images relative to the Corresponding slices of the 3D mu-CT reconstruction. Mass channels corresponding to the isotopes of a single element were merged to improve the signal-to-noise ratios within the elemental images. In order to aid the visual interpretation of the data, LA-ICP-TOEMS data wete projected onto the mu-CT voxels representing tissue. Additionally, the image resolution and elemental sensitivity were compared to those obtained with synchrotron radiation based 3D confocal mu-X-ray fluorescence imaging upon a chemically fixed and air-dried C. dubia specimen.},
  author       = {Van Malderen, Stijn and Laforce, Brecht and Van Acker, Thibaut and Nys, Charlotte and De Rijcke, Maarten and De Rycke, Riet and De Bruyne, Michiel and Boone, Matthieu and De Schamphelaere, Karel and Borovinskaya, Olga and De Samber, Bj{\"o}rn and Vincze, Laszlo and Vanhaecke, Frank},
  issn         = {0003-2700},
  journal      = {ANALYTICAL CHEMISTRY},
  keyword      = {DAPHNIA-MAGNA,GEOLOGICAL SAMPLES,MIXTURE TOXICITY,HIGH-RESOLUTION,HIGH-SPEED,ZINC,NICKEL,ZN,INSTRUMENTATION,ASSIMILATION},
  language     = {eng},
  number       = {7},
  pages        = {4161--4168},
  title        = {Three-dimensional reconstruction of the tissue-specific multielemental distribution within Ceriodaphnia dubia via multimodal registration using laser ablation ICP-mass spectrometry and X-ray spectroscopic techniques},
  url          = {http://dx.doi.org/10.1021/acs.analchem.7b00111},
  volume       = {89},
  year         = {2017},
}

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