Programmed cell death-1 expression correlates with disease severity and IL-5 in chronic rhinosinusitis with nasal polyps
- Author
- I Kortekaas Krohn, S Bobic, J Dooley, Feng Lan (UGent) , Nan Zhang (UGent) , Claus Bachert (UGent) , B Steelant, DM Bullens, A Liston, JL Ceuppens, SF Seys and PW Hellings
- Organization
- Abstract
- Background: Programmed cell death-1 (PD-1) is a negative regulator of T-cell responses. Expression of PD-1 and its ligands PD-L1 and PD-L2 in chronic rhinosinusitis with nasal polyps (CRSwNP) is poorly studied. Methods: Expression of PD-1, PD-L1, PD-L2, TGF-beta, IL-5, and IL-10 mRNA was measured by real-time quantitative PCR on tissue homogenates of patients with CRSwNP (n = 21) and healthy controls (n = 21) and on primary epithelial cells. Disease severity was scored using the Lund-Mackay scores of maxillofacial computed tomography (CT) scans. Expression of PD-1 and PD-L1/L2 was evaluated at the cellular and tissue levels (n = 6) by flow cytometry and immunohistochemistry. Results: Programmed cell death-1 mRNA expression was increased in tissue homogenates from patients with CRSwNP compared with controls, irrespective of the atopy status. Importantly, expression of PD-1 correlated with the total CT scan scores (r = 0.5, P = 0.02). Additionally, a significant association was found between PD-1 mRNA and expression of IL-5 mRNA in control nasal tissue (r = 0.95, P < 0.0001) and in CRSwNP (r = 0.63, P = 0.002). PD-1 was expressed on different subsets of T cells and CD11b(-) dendritic cells. Both PD-1 and its ligands were expressed on primary epithelial cells from control nasal tissue and nasal polyp tissue. Conclusions: Higher PD-1 expression was found in CRSwNP than in nasal tissue from controls. This was associated with disease severity and tissue IL-5 expression but unrelated to the patients' atopy status.
- Keywords
- chronic rhinosinusitis, interleukin-5, nasal polyps, programmed cell death-1, radiologic severity, AIRWAY EPITHELIAL-CELLS, ALLERGIC DISEASES, B7 FAMILY, T-CELLS, IN-VIVO, ASTHMA, PD-1, ACTIVATION, INFECTION, RECEPTOR
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Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8513809
- MLA
- Kortekaas Krohn, I et al. “Programmed Cell Death-1 Expression Correlates with Disease Severity and IL-5 in Chronic Rhinosinusitis with Nasal Polyps.” ALLERGY 72.6 (2017): 985–993. Print.
- APA
- Kortekaas Krohn, I., Bobic, S., Dooley, J., Lan, F., Zhang, N., Bachert, C., Steelant, B., et al. (2017). Programmed cell death-1 expression correlates with disease severity and IL-5 in chronic rhinosinusitis with nasal polyps. ALLERGY, 72(6), 985–993.
- Chicago author-date
- Kortekaas Krohn, I, S Bobic, J Dooley, Feng Lan, Nan Zhang, Claus Bachert, B Steelant, et al. 2017. “Programmed Cell Death-1 Expression Correlates with Disease Severity and IL-5 in Chronic Rhinosinusitis with Nasal Polyps.” Allergy 72 (6): 985–993.
- Chicago author-date (all authors)
- Kortekaas Krohn, I, S Bobic, J Dooley, Feng Lan, Nan Zhang, Claus Bachert, B Steelant, DM Bullens, A Liston, JL Ceuppens, SF Seys, and PW Hellings. 2017. “Programmed Cell Death-1 Expression Correlates with Disease Severity and IL-5 in Chronic Rhinosinusitis with Nasal Polyps.” Allergy 72 (6): 985–993.
- Vancouver
- 1.Kortekaas Krohn I, Bobic S, Dooley J, Lan F, Zhang N, Bachert C, et al. Programmed cell death-1 expression correlates with disease severity and IL-5 in chronic rhinosinusitis with nasal polyps. ALLERGY. 2017;72(6):985–93.
- IEEE
- [1]I. Kortekaas Krohn et al., “Programmed cell death-1 expression correlates with disease severity and IL-5 in chronic rhinosinusitis with nasal polyps,” ALLERGY, vol. 72, no. 6, pp. 985–993, 2017.
@article{8513809,
abstract = {Background: Programmed cell death-1 (PD-1) is a negative regulator of T-cell responses. Expression of PD-1 and its ligands PD-L1 and PD-L2 in chronic rhinosinusitis with nasal polyps (CRSwNP) is poorly studied.
Methods: Expression of PD-1, PD-L1, PD-L2, TGF-beta, IL-5, and IL-10 mRNA was measured by real-time quantitative PCR on tissue homogenates of patients with CRSwNP (n = 21) and healthy controls (n = 21) and on primary epithelial cells. Disease severity was scored using the Lund-Mackay scores of maxillofacial computed tomography (CT) scans. Expression of PD-1 and PD-L1/L2 was evaluated at the cellular and tissue levels (n = 6) by flow cytometry and immunohistochemistry.
Results: Programmed cell death-1 mRNA expression was increased in tissue homogenates from patients with CRSwNP compared with controls, irrespective of the atopy status. Importantly, expression of PD-1 correlated with the total CT scan scores (r = 0.5, P = 0.02). Additionally, a significant association was found between PD-1 mRNA and expression of IL-5 mRNA in control nasal tissue (r = 0.95, P < 0.0001) and in CRSwNP (r = 0.63, P = 0.002). PD-1 was expressed on different subsets of T cells and CD11b(-) dendritic cells. Both PD-1 and its ligands were expressed on primary epithelial cells from control nasal tissue and nasal polyp tissue.
Conclusions: Higher PD-1 expression was found in CRSwNP than in nasal tissue from controls. This was associated with disease severity and tissue IL-5 expression but unrelated to the patients' atopy status.},
author = {Kortekaas Krohn, I and Bobic, S and Dooley, J and Lan, Feng and Zhang, Nan and Bachert, Claus and Steelant, B and Bullens, DM and Liston, A and Ceuppens, JL and Seys, SF and Hellings, PW},
issn = {0105-4538},
journal = {ALLERGY},
keywords = {chronic rhinosinusitis,interleukin-5,nasal polyps,programmed cell death-1,radiologic severity,AIRWAY EPITHELIAL-CELLS,ALLERGIC DISEASES,B7 FAMILY,T-CELLS,IN-VIVO,ASTHMA,PD-1,ACTIVATION,INFECTION,RECEPTOR},
language = {eng},
number = {6},
pages = {985--993},
title = {Programmed cell death-1 expression correlates with disease severity and IL-5 in chronic rhinosinusitis with nasal polyps},
url = {http://dx.doi.org/10.1111/all.13136},
volume = {72},
year = {2017},
}
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