Programmed cell death-1 expression correlates with disease severity and IL-5 in chronic rhinosinusitis with nasal polyps
(2017) ALLERGY. 72(6). p.985-993- abstract
- Background: Programmed cell death-1 (PD-1) is a negative regulator of T-cell responses. Expression of PD-1 and its ligands PD-L1 and PD-L2 in chronic rhinosinusitis with nasal polyps (CRSwNP) is poorly studied. Methods: Expression of PD-1, PD-L1, PD-L2, TGF-beta, IL-5, and IL-10 mRNA was measured by real-time quantitative PCR on tissue homogenates of patients with CRSwNP (n = 21) and healthy controls (n = 21) and on primary epithelial cells. Disease severity was scored using the Lund-Mackay scores of maxillofacial computed tomography (CT) scans. Expression of PD-1 and PD-L1/L2 was evaluated at the cellular and tissue levels (n = 6) by flow cytometry and immunohistochemistry. Results: Programmed cell death-1 mRNA expression was increased in tissue homogenates from patients with CRSwNP compared with controls, irrespective of the atopy status. Importantly, expression of PD-1 correlated with the total CT scan scores (r = 0.5, P = 0.02). Additionally, a significant association was found between PD-1 mRNA and expression of IL-5 mRNA in control nasal tissue (r = 0.95, P < 0.0001) and in CRSwNP (r = 0.63, P = 0.002). PD-1 was expressed on different subsets of T cells and CD11b(-) dendritic cells. Both PD-1 and its ligands were expressed on primary epithelial cells from control nasal tissue and nasal polyp tissue. Conclusions: Higher PD-1 expression was found in CRSwNP than in nasal tissue from controls. This was associated with disease severity and tissue IL-5 expression but unrelated to the patients' atopy status.
Please use this url to cite or link to this publication:
http://hdl.handle.net/1854/LU-8513809
- author
- I Kortekaas Krohn, S Bobic, J Dooley, Feng Lan, Nan Zhang UGent, Claus Bachert UGent, B Steelant, DM Bullens, A Liston, JL Ceuppens, SF Seys and PW Hellings
- organization
- year
- 2017
- type
- journalArticle (original)
- publication status
- published
- subject
- keyword
- chronic rhinosinusitis, interleukin-5, nasal polyps, programmed cell death-1, radiologic severity, AIRWAY EPITHELIAL-CELLS, ALLERGIC DISEASES, B7 FAMILY, T-CELLS, IN-VIVO, ASTHMA, PD-1, ACTIVATION, INFECTION, RECEPTOR
- journal title
- ALLERGY
- Allergy
- volume
- 72
- issue
- 6
- pages
- 985 - 993
- Web of Science type
- Article
- Web of Science id
- 000402834300016
- ISSN
- 0105-4538
- DOI
- 10.1111/all.13136
- language
- English
- UGent publication?
- yes
- classification
- A1
- copyright statement
- I have transferred the copyright for this publication to the publisher
- id
- 8513809
- handle
- http://hdl.handle.net/1854/LU-8513809
- date created
- 2017-03-10 09:46:43
- date last changed
- 2017-09-06 14:00:35
@article{8513809, abstract = {Background: Programmed cell death-1 (PD-1) is a negative regulator of T-cell responses. Expression of PD-1 and its ligands PD-L1 and PD-L2 in chronic rhinosinusitis with nasal polyps (CRSwNP) is poorly studied. Methods: Expression of PD-1, PD-L1, PD-L2, TGF-beta, IL-5, and IL-10 mRNA was measured by real-time quantitative PCR on tissue homogenates of patients with CRSwNP (n = 21) and healthy controls (n = 21) and on primary epithelial cells. Disease severity was scored using the Lund-Mackay scores of maxillofacial computed tomography (CT) scans. Expression of PD-1 and PD-L1/L2 was evaluated at the cellular and tissue levels (n = 6) by flow cytometry and immunohistochemistry. Results: Programmed cell death-1 mRNA expression was increased in tissue homogenates from patients with CRSwNP compared with controls, irrespective of the atopy status. Importantly, expression of PD-1 correlated with the total CT scan scores (r = 0.5, P = 0.02). Additionally, a significant association was found between PD-1 mRNA and expression of IL-5 mRNA in control nasal tissue (r = 0.95, P {\textlangle} 0.0001) and in CRSwNP (r = 0.63, P = 0.002). PD-1 was expressed on different subsets of T cells and CD11b(-) dendritic cells. Both PD-1 and its ligands were expressed on primary epithelial cells from control nasal tissue and nasal polyp tissue. Conclusions: Higher PD-1 expression was found in CRSwNP than in nasal tissue from controls. This was associated with disease severity and tissue IL-5 expression but unrelated to the patients' atopy status.}, author = {Kortekaas Krohn, I and Bobic, S and Dooley, J and Lan, Feng and Zhang, Nan and Bachert, Claus and Steelant, B and Bullens, DM and Liston, A and Ceuppens, JL and Seys, SF and Hellings, PW}, issn = {0105-4538}, journal = {ALLERGY}, keyword = {chronic rhinosinusitis,interleukin-5,nasal polyps,programmed cell death-1,radiologic severity,AIRWAY EPITHELIAL-CELLS,ALLERGIC DISEASES,B7 FAMILY,T-CELLS,IN-VIVO,ASTHMA,PD-1,ACTIVATION,INFECTION,RECEPTOR}, language = {eng}, number = {6}, pages = {985--993}, title = {Programmed cell death-1 expression correlates with disease severity and IL-5 in chronic rhinosinusitis with nasal polyps}, url = {http://dx.doi.org/10.1111/all.13136}, volume = {72}, year = {2017}, }
- Chicago
- Kortekaas Krohn, I, S Bobic, J Dooley, Feng Lan, Nan Zhang, Claus Bachert, B Steelant, et al. 2017. “Programmed Cell Death-1 Expression Correlates with Disease Severity and IL-5 in Chronic Rhinosinusitis with Nasal Polyps.” Allergy 72 (6): 985–993.
- APA
- Kortekaas Krohn, I., Bobic, S., Dooley, J., Lan, F., Zhang, N., Bachert, C., Steelant, B., et al. (2017). Programmed cell death-1 expression correlates with disease severity and IL-5 in chronic rhinosinusitis with nasal polyps. ALLERGY, 72(6), 985–993.
- Vancouver
- 1.Kortekaas Krohn I, Bobic S, Dooley J, Lan F, Zhang N, Bachert C, et al. Programmed cell death-1 expression correlates with disease severity and IL-5 in chronic rhinosinusitis with nasal polyps. ALLERGY. 2017;72(6):985–93.
- MLA
- Kortekaas Krohn, I, S Bobic, J Dooley, et al. “Programmed Cell Death-1 Expression Correlates with Disease Severity and IL-5 in Chronic Rhinosinusitis with Nasal Polyps.” ALLERGY 72.6 (2017): 985–993. Print.