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Characterization and genetic diversity of causal agent of stone fruit bacterial canker Pseudomonas cerasi, a new pathogen of cherry

Monika Kałużna, Anne Willems UGent, Joël F Pothier, Michela Ruinelli, Piotr Sobiczewski and Joanna Puławska (2016) Acta Horticulturae. 1149. p.9-14
abstract
Based on phenotypic tests, 49 out of 168 isolates of Pseudomonas syringae obtained from various organs of diseased tissue originating from various regions in Poland, were identified as P. syringae pv. morsprunorum race 1 (Psm1), 10 as race 2 of this pathovar (Psm2), 53 as pathovar syringae (Pss) and 56 as an atypical taxon. The pathogenicity test on immature sweet cherry fruits divided the tested strains into two groups: one with isolates causing black brown necrosis and the second containing isolates inducing water soaked superficial lesions. Phenotypic and genetic studies on toxins production by bacteria showed that all Pss produced syringomycin. However, only some Psm1 isolates have the gene for coronatine production. All strains belonging to Psm2 possessed genes encoding yersiniabactin. Results of genetic analyses (rep-PCRs, PCR MP) confirmed the homogeneity of isolates within pathovar morsprunorum and the atypical taxon and revealed diversity within pathovar syringae. A detailed polyphasic approach including phenotypic and genetic characterization applied for eight strains from the atypical taxon showed that the strains represent a novel species of the genus Pseudomonas for which Pseudomonas cerasi sp. nov. (non Griffin, 1911) is proposed.
Please use this url to cite or link to this publication:
author
organization
year
type
conference (proceedingsPaper)
publication status
published
subject
keyword
SYRINGAE PV SYRINGAE, GENUS PSEUDOMONAS, WILD CHERRY, PCR, STRAINS, PATHOVARS, IDENTIFICATION, DNA, DISCRIMINATION, MORSPRUNORUM, Pseudomonas sp., bacterial canker, diagnosis, diversity, DNA-DNA, hybridization, MLSA, MALDI TOF MS
in
Acta Horticulturae
Acta Hortic.
editor
H Ozakatan, A Akkopru and N Hustun
volume
1149
issue title
II International workshop on bacterial diseases of stone fruits and nuts
pages
9 - 14
publisher
International Society for Horticultural Science (ISHS)
place of publication
Leuven, Belgium
conference name
2nd International workshop on Bacterial Diseases of Stone Fruits and Nuts
conference location
Izmir, Turkey
conference start
2015-04-21
conference end
2015-04-24
Web of Science type
Proceedings Paper
Web of Science id
000392713600002
ISSN
0567-7572
ISBN
9789462611405
DOI
10.17660/ActaHortic.2016.1149.2
language
English
UGent publication?
yes
classification
P1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8513451
handle
http://hdl.handle.net/1854/LU-8513451
date created
2017-03-09 09:17:12
date last changed
2017-11-21 14:36:43
@inproceedings{8513451,
  abstract     = {Based on phenotypic tests, 49 out of 168 isolates of Pseudomonas syringae obtained from various organs of diseased tissue originating from various regions in Poland, were identified as P. syringae pv. morsprunorum race 1 (Psm1), 10 as race 2 of this pathovar (Psm2), 53 as pathovar syringae (Pss) and 56 as an atypical taxon. The pathogenicity test on immature sweet cherry fruits divided the tested strains into two groups: one with isolates causing black brown necrosis and the second containing isolates inducing water soaked superficial lesions. Phenotypic and genetic studies on toxins production by bacteria showed that all Pss produced syringomycin. However, only some Psm1 isolates have the gene for coronatine production. All strains belonging to Psm2 possessed genes encoding yersiniabactin. Results of genetic analyses (rep-PCRs, PCR MP) confirmed the homogeneity of isolates within pathovar morsprunorum and the atypical taxon and revealed diversity within pathovar syringae. A detailed polyphasic approach including phenotypic and genetic characterization applied for eight strains from the atypical taxon showed that the strains represent a novel species of the genus Pseudomonas for which Pseudomonas cerasi sp. nov. (non Griffin, 1911) is proposed.},
  author       = {Ka\unmatched{0142}u\.{z}na, Monika and Willems, Anne and Pothier, Jo{\"e}l F and Ruinelli, Michela and Sobiczewski, Piotr and Pu\unmatched{0142}awska, Joanna},
  booktitle    = {Acta Horticulturae},
  editor       = {Ozakatan, H and Akkopru, A and Hustun, N},
  isbn         = {9789462611405},
  issn         = {0567-7572},
  keyword      = {SYRINGAE PV SYRINGAE,GENUS PSEUDOMONAS,WILD CHERRY,PCR,STRAINS,PATHOVARS,IDENTIFICATION,DNA,DISCRIMINATION,MORSPRUNORUM,Pseudomonas sp.,bacterial canker,diagnosis,diversity,DNA-DNA,hybridization,MLSA,MALDI TOF MS},
  language     = {eng},
  location     = {Izmir, Turkey},
  pages        = {9--14},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {Characterization and genetic diversity of causal agent of stone fruit bacterial canker Pseudomonas cerasi, a new pathogen of cherry},
  url          = {http://dx.doi.org/10.17660/ActaHortic.2016.1149.2},
  volume       = {1149},
  year         = {2016},
}

Chicago
Kałużna, Monika, Anne Willems, Joël F Pothier, Michela Ruinelli, Piotr Sobiczewski, and Joanna Puławska. 2016. “Characterization and Genetic Diversity of Causal Agent of Stone Fruit Bacterial Canker Pseudomonas Cerasi, a New Pathogen of Cherry.” In Acta Horticulturae, ed. H Ozakatan, A Akkopru, and N Hustun, 1149:9–14. Leuven, Belgium: International Society for Horticultural Science (ISHS).
APA
Kałużna, M., Willems, A., Pothier, J. F., Ruinelli, M., Sobiczewski, P., & Puławska, J. (2016). Characterization and genetic diversity of causal agent of stone fruit bacterial canker Pseudomonas cerasi, a new pathogen of cherry. In H Ozakatan, A. Akkopru, & N. Hustun (Eds.), Acta Horticulturae (Vol. 1149, pp. 9–14). Presented at the 2nd International workshop on Bacterial Diseases of Stone Fruits and Nuts, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Kałużna M, Willems A, Pothier JF, Ruinelli M, Sobiczewski P, Puławska J. Characterization and genetic diversity of causal agent of stone fruit bacterial canker Pseudomonas cerasi, a new pathogen of cherry. In: Ozakatan H, Akkopru A, Hustun N, editors. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2016. p. 9–14.
MLA
Kałużna, Monika, Anne Willems, Joël F Pothier, et al. “Characterization and Genetic Diversity of Causal Agent of Stone Fruit Bacterial Canker Pseudomonas Cerasi, a New Pathogen of Cherry.” Acta Horticulturae. Ed. H Ozakatan, A Akkopru, & N Hustun. Vol. 1149. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2016. 9–14. Print.