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Characterization and genetic diversity of causal agent of stone fruit bacterial canker Pseudomonas cerasi, a new pathogen of cherry

(2016) Acta Horticulturae. 1149. p.9-14
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Abstract
Based on phenotypic tests, 49 out of 168 isolates of Pseudomonas syringae obtained from various organs of diseased tissue originating from various regions in Poland, were identified as P. syringae pv. morsprunorum race 1 (Psm1), 10 as race 2 of this pathovar (Psm2), 53 as pathovar syringae (Pss) and 56 as an atypical taxon. The pathogenicity test on immature sweet cherry fruits divided the tested strains into two groups: one with isolates causing black brown necrosis and the second containing isolates inducing water soaked superficial lesions. Phenotypic and genetic studies on toxins production by bacteria showed that all Pss produced syringomycin. However, only some Psm1 isolates have the gene for coronatine production. All strains belonging to Psm2 possessed genes encoding yersiniabactin. Results of genetic analyses (rep-PCRs, PCR MP) confirmed the homogeneity of isolates within pathovar morsprunorum and the atypical taxon and revealed diversity within pathovar syringae. A detailed polyphasic approach including phenotypic and genetic characterization applied for eight strains from the atypical taxon showed that the strains represent a novel species of the genus Pseudomonas for which Pseudomonas cerasi sp. nov. (non Griffin, 1911) is proposed.
Keywords
SYRINGAE PV SYRINGAE, GENUS PSEUDOMONAS, WILD CHERRY, PCR, STRAINS, PATHOVARS, IDENTIFICATION, DNA, DISCRIMINATION, MORSPRUNORUM, Pseudomonas sp., bacterial canker, diagnosis, diversity, DNA-DNA, hybridization, MLSA, MALDI TOF MS

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Chicago
Kałużna, Monika, Anne Willems, Joël F Pothier, Michela Ruinelli, Piotr Sobiczewski, and Joanna Puławska. 2016. “Characterization and Genetic Diversity of Causal Agent of Stone Fruit Bacterial Canker Pseudomonas Cerasi, a New Pathogen of Cherry.” In Acta Horticulturae, ed. H Ozakatan, A Akkopru, and N Hustun, 1149:9–14. Leuven, Belgium: International Society for Horticultural Science (ISHS).
APA
Kałużna, M., Willems, A., Pothier, J. F., Ruinelli, M., Sobiczewski, P., & Puławska, J. (2016). Characterization and genetic diversity of causal agent of stone fruit bacterial canker Pseudomonas cerasi, a new pathogen of cherry. In H Ozakatan, A. Akkopru, & N. Hustun (Eds.), Acta Horticulturae (Vol. 1149, pp. 9–14). Presented at the 2nd International workshop on Bacterial Diseases of Stone Fruits and Nuts, Leuven, Belgium: International Society for Horticultural Science (ISHS).
Vancouver
1.
Kałużna M, Willems A, Pothier JF, Ruinelli M, Sobiczewski P, Puławska J. Characterization and genetic diversity of causal agent of stone fruit bacterial canker Pseudomonas cerasi, a new pathogen of cherry. In: Ozakatan H, Akkopru A, Hustun N, editors. Acta Horticulturae. Leuven, Belgium: International Society for Horticultural Science (ISHS); 2016. p. 9–14.
MLA
Kałużna, Monika, Anne Willems, Joël F Pothier, et al. “Characterization and Genetic Diversity of Causal Agent of Stone Fruit Bacterial Canker Pseudomonas Cerasi, a New Pathogen of Cherry.” Acta Horticulturae. Ed. H Ozakatan, A Akkopru, & N Hustun. Vol. 1149. Leuven, Belgium: International Society for Horticultural Science (ISHS), 2016. 9–14. Print.
@inproceedings{8513451,
  abstract     = {Based on phenotypic tests, 49 out of 168 isolates of Pseudomonas syringae obtained from various organs of diseased tissue originating from various regions in Poland, were identified as P. syringae pv. morsprunorum race 1 (Psm1), 10 as race 2 of this pathovar (Psm2), 53 as pathovar syringae (Pss) and 56 as an atypical taxon. The pathogenicity test on immature sweet cherry fruits divided the tested strains into two groups: one with isolates causing black brown necrosis and the second containing isolates inducing water soaked superficial lesions. Phenotypic and genetic studies on toxins production by bacteria showed that all Pss produced syringomycin. However, only some Psm1 isolates have the gene for coronatine production. All strains belonging to Psm2 possessed genes encoding yersiniabactin. Results of genetic analyses (rep-PCRs, PCR MP) confirmed the homogeneity of isolates within pathovar morsprunorum and the atypical taxon and revealed diversity within pathovar syringae. A detailed polyphasic approach including phenotypic and genetic characterization applied for eight strains from the atypical taxon showed that the strains represent a novel species of the genus Pseudomonas for which Pseudomonas cerasi sp. nov. (non Griffin, 1911) is proposed.},
  author       = {Ka\unmatched{0142}u\.{z}na, Monika and Willems, Anne and Pothier, Jo{\"e}l F and Ruinelli, Michela and Sobiczewski, Piotr and Pu\unmatched{0142}awska, Joanna},
  booktitle    = {Acta Horticulturae},
  editor       = {Ozakatan, H and Akkopru, A and Hustun, N},
  isbn         = {9789462611405},
  issn         = {0567-7572},
  keyword      = {SYRINGAE PV SYRINGAE,GENUS PSEUDOMONAS,WILD CHERRY,PCR,STRAINS,PATHOVARS,IDENTIFICATION,DNA,DISCRIMINATION,MORSPRUNORUM,Pseudomonas sp.,bacterial canker,diagnosis,diversity,DNA-DNA,hybridization,MLSA,MALDI TOF MS},
  language     = {eng},
  location     = {Izmir, Turkey},
  pages        = {9--14},
  publisher    = {International Society for Horticultural Science (ISHS)},
  title        = {Characterization and genetic diversity of causal agent of stone fruit bacterial canker Pseudomonas cerasi, a new pathogen of cherry},
  url          = {http://dx.doi.org/10.17660/ActaHortic.2016.1149.2},
  volume       = {1149},
  year         = {2016},
}

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