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Forensic SNP genotyping using nanopore MinION sequencing

Senne Cornelis (UGent) , Yannick Gansemans (UGent) , Lieselot Deleye (UGent) , Dieter Deforce (UGent) and Filip Van Nieuwerburgh (UGent)
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Abstract
One of the latest developments in next generation sequencing is the Oxford Nanopore Technologies' (ONT) MinION nanopore sequencer. We studied the applicability of this system to perform forensic genotyping of the forensic female DNA standard 9947 A using the 52 SNP-plex assay developed by the SNPforID consortium. All but one of the loci were correctly genotyped. Several SNP loci were identified as problematic for correct and robust genotyping using nanopore sequencing. All these loci contained homopolymers in the sequence flanking the forensic SNP and most of them were already reported as problematic in studies using other sequencing technologies. When these problematic loci are avoided, correct forensic genotyping using nanopore sequencing is technically feasible.
Keywords
SINGLE NUCLEOTIDE POLYMORPHISMS, HUMAN IDENTIFICATION, ASSAY

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Citation

Please use this url to cite or link to this publication:

Chicago
Cornelis, Senne, Yannick Gansemans, Lieselot Deleye, Dieter Deforce, and Filip Van Nieuwerburgh. 2017. “Forensic SNP Genotyping Using Nanopore MinION Sequencing.” Scientific Reports 7.
APA
Cornelis, Senne, Gansemans, Y., Deleye, L., Deforce, D., & Van Nieuwerburgh, F. (2017). Forensic SNP genotyping using nanopore MinION sequencing. SCIENTIFIC REPORTS, 7.
Vancouver
1.
Cornelis S, Gansemans Y, Deleye L, Deforce D, Van Nieuwerburgh F. Forensic SNP genotyping using nanopore MinION sequencing. SCIENTIFIC REPORTS. 2017;7.
MLA
Cornelis, Senne, Yannick Gansemans, Lieselot Deleye, et al. “Forensic SNP Genotyping Using Nanopore MinION Sequencing.” SCIENTIFIC REPORTS 7 (2017): n. pag. Print.
@article{8512083,
  abstract     = {One of the latest developments in next generation sequencing is the Oxford Nanopore Technologies' (ONT) MinION nanopore sequencer. We studied the applicability of this system to perform forensic genotyping of the forensic female DNA standard 9947 A using the 52 SNP-plex assay developed by the SNPforID consortium. All but one of the loci were correctly genotyped. Several SNP loci were identified as problematic for correct and robust genotyping using nanopore sequencing. All these loci contained homopolymers in the sequence flanking the forensic SNP and most of them were already reported as problematic in studies using other sequencing technologies. When these problematic loci are avoided, correct forensic genotyping using nanopore sequencing is technically feasible.},
  articleno    = {41759},
  author       = {Cornelis, Senne and Gansemans, Yannick and Deleye, Lieselot and Deforce, Dieter and Van Nieuwerburgh, Filip},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keyword      = {SINGLE NUCLEOTIDE POLYMORPHISMS,HUMAN IDENTIFICATION,ASSAY},
  language     = {eng},
  pages        = {5},
  title        = {Forensic SNP genotyping using nanopore MinION sequencing},
  url          = {http://dx.doi.org/10.1038/srep41759},
  volume       = {7},
  year         = {2017},
}

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