Ghent University Academic Bibliography

Advanced

Forensic SNP genotyping using nanopore MinION sequencing

Senne Cornelis UGent, Yannick Gansemans UGent, Lieselot Deleye UGent, Dieter Deforce UGent and Filip Van Nieuwerburgh UGent (2017) SCIENTIFIC REPORTS. 7.
abstract
One of the latest developments in next generation sequencing is the Oxford Nanopore Technologies' (ONT) MinION nanopore sequencer. We studied the applicability of this system to perform forensic genotyping of the forensic female DNA standard 9947 A using the 52 SNP-plex assay developed by the SNPforID consortium. All but one of the loci were correctly genotyped. Several SNP loci were identified as problematic for correct and robust genotyping using nanopore sequencing. All these loci contained homopolymers in the sequence flanking the forensic SNP and most of them were already reported as problematic in studies using other sequencing technologies. When these problematic loci are avoided, correct forensic genotyping using nanopore sequencing is technically feasible.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SINGLE NUCLEOTIDE POLYMORPHISMS, HUMAN IDENTIFICATION, ASSAY
journal title
SCIENTIFIC REPORTS
Sci. Rep.
volume
7
article number
41759
pages
5 pages
Web of Science type
Article
Web of Science id
000393549400001
ISSN
2045-2322
DOI
10.1038/srep41759
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8512083
handle
http://hdl.handle.net/1854/LU-8512083
date created
2017-03-02 10:45:42
date last changed
2017-03-03 12:58:23
@article{8512083,
  abstract     = {One of the latest developments in next generation sequencing is the Oxford Nanopore Technologies' (ONT) MinION nanopore sequencer. We studied the applicability of this system to perform forensic genotyping of the forensic female DNA standard 9947 A using the 52 SNP-plex assay developed by the SNPforID consortium. All but one of the loci were correctly genotyped. Several SNP loci were identified as problematic for correct and robust genotyping using nanopore sequencing. All these loci contained homopolymers in the sequence flanking the forensic SNP and most of them were already reported as problematic in studies using other sequencing technologies. When these problematic loci are avoided, correct forensic genotyping using nanopore sequencing is technically feasible.},
  articleno    = {41759},
  author       = {Cornelis, Senne and Gansemans, Yannick and Deleye, Lieselot and Deforce, Dieter and Van Nieuwerburgh, Filip},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keyword      = {SINGLE NUCLEOTIDE POLYMORPHISMS,HUMAN IDENTIFICATION,ASSAY},
  language     = {eng},
  pages        = {5},
  title        = {Forensic SNP genotyping using nanopore MinION sequencing},
  url          = {http://dx.doi.org/10.1038/srep41759},
  volume       = {7},
  year         = {2017},
}

Chicago
Cornelis, Senne, Yannick Gansemans, Lieselot Deleye, Dieter Deforce, and Filip Van Nieuwerburgh. 2017. “Forensic SNP Genotyping Using Nanopore MinION Sequencing.” Scientific Reports 7.
APA
Cornelis, Senne, Gansemans, Y., Deleye, L., Deforce, D., & Van Nieuwerburgh, F. (2017). Forensic SNP genotyping using nanopore MinION sequencing. SCIENTIFIC REPORTS, 7.
Vancouver
1.
Cornelis S, Gansemans Y, Deleye L, Deforce D, Van Nieuwerburgh F. Forensic SNP genotyping using nanopore MinION sequencing. SCIENTIFIC REPORTS. 2017;7.
MLA
Cornelis, Senne, Yannick Gansemans, Lieselot Deleye, et al. “Forensic SNP Genotyping Using Nanopore MinION Sequencing.” SCIENTIFIC REPORTS 7 (2017): n. pag. Print.