Murine models of allergic asthma
- Author
- Eline Haspeslagh (UGent) , Nincy Debeuf (UGent) , Hamida Hammad (UGent) and Bart Lambrecht (UGent)
- Organization
- Abstract
- Allergic asthma is a heterogeneous inflammatory lung disease affecting millions of people worldwide and with a steadily increasing incidence. Mouse models have been of utmost importance in uncovering key inflammatory cell types, cytokines, and pathways in the development and maintenance of allergic asthma. Historically, the mainstay in experimental asthma research was sensitizing rodents to the model protein antigen ovalbumin (OVA) with the pro-Th2 adjuvant aluminum hydroxide, followed by repetitive OVA exposures to the airways to initiate a Th2-skewed adaptive immune response leading to eosinophilic airway inflammation and airway hyperreactivity (AHR). In the last 5 years, OVA is often replaced by naturally occurring allergens such as house dust mite (HDM) or cockroach extracts, but the principle of first sensitizing and then repetitively challenging mice with the same antigen is unchanged. Here, we describe an often used and relevant HDM-based protocol to establish acute allergic asthma, and the methods we have developed to rapidly analyze inflammatory cell infiltration in the bronchalveolar lavage fluid by flow cytometry. Moreover, we explain the methods to restimulate T cells from lung-draining mediastinal lymph nodes with HDM to allow the measurement of cytokine secretion profiles of allergen reactive T cells.
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8511457
- MLA
- Haspeslagh, Eline, et al. “Murine Models of Allergic Asthma.” Inflammation : Methods and Protocols, edited by Björn E Clausen and Jon D Laman, vol. 1559, Springer, 2017, pp. 121–36, doi:10.1007/978-1-4939-6786-5_10.
- APA
- Haspeslagh, E., Debeuf, N., Hammad, H., & Lambrecht, B. (2017). Murine models of allergic asthma. In B. E. Clausen & J. D. Laman (Eds.), Inflammation : methods and protocols (Vol. 1559, pp. 121–136). https://doi.org/10.1007/978-1-4939-6786-5_10
- Chicago author-date
- Haspeslagh, Eline, Nincy Debeuf, Hamida Hammad, and Bart Lambrecht. 2017. “Murine Models of Allergic Asthma.” In Inflammation : Methods and Protocols, edited by Björn E Clausen and Jon D Laman, 1559:121–36. New York, NY, USA: Springer. https://doi.org/10.1007/978-1-4939-6786-5_10.
- Chicago author-date (all authors)
- Haspeslagh, Eline, Nincy Debeuf, Hamida Hammad, and Bart Lambrecht. 2017. “Murine Models of Allergic Asthma.” In Inflammation : Methods and Protocols, ed by. Björn E Clausen and Jon D Laman, 1559:121–136. New York, NY, USA: Springer. doi:10.1007/978-1-4939-6786-5_10.
- Vancouver
- 1.Haspeslagh E, Debeuf N, Hammad H, Lambrecht B. Murine models of allergic asthma. In: Clausen BE, Laman JD, editors. Inflammation : methods and protocols. New York, NY, USA: Springer; 2017. p. 121–36.
- IEEE
- [1]E. Haspeslagh, N. Debeuf, H. Hammad, and B. Lambrecht, “Murine models of allergic asthma,” in Inflammation : methods and protocols, vol. 1559, B. E. Clausen and J. D. Laman, Eds. New York, NY, USA: Springer, 2017, pp. 121–136.
@incollection{8511457,
abstract = {{Allergic asthma is a heterogeneous inflammatory lung disease affecting millions of people worldwide and with a steadily increasing incidence. Mouse models have been of utmost importance in uncovering key inflammatory cell types, cytokines, and pathways in the development and maintenance of allergic asthma. Historically, the mainstay in experimental asthma research was sensitizing rodents to the model protein antigen ovalbumin (OVA) with the pro-Th2 adjuvant aluminum hydroxide, followed by repetitive OVA exposures to the airways to initiate a Th2-skewed adaptive immune response leading to eosinophilic airway inflammation and airway hyperreactivity (AHR). In the last 5 years, OVA is often replaced by naturally occurring allergens such as house dust mite (HDM) or cockroach extracts, but the principle of first sensitizing and then repetitively challenging mice with the same antigen is unchanged. Here, we describe an often used and relevant HDM-based protocol to establish acute allergic asthma, and the methods we have developed to rapidly analyze inflammatory cell infiltration in the bronchalveolar lavage fluid by flow cytometry. Moreover, we explain the methods to restimulate T cells from lung-draining mediastinal lymph nodes with HDM to allow the measurement of cytokine secretion profiles of allergen reactive T cells.}},
author = {{Haspeslagh, Eline and Debeuf, Nincy and Hammad, Hamida and Lambrecht, Bart}},
booktitle = {{Inflammation : methods and protocols}},
editor = {{Clausen, Björn E and Laman, Jon D}},
isbn = {{9781493967841}},
issn = {{1064-3745}},
language = {{eng}},
pages = {{121--136}},
publisher = {{Springer}},
series = {{Methods in Molecular Biology}},
title = {{Murine models of allergic asthma}},
url = {{http://doi.org/10.1007/978-1-4939-6786-5_10}},
volume = {{1559}},
year = {{2017}},
}
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