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Murine models of allergic asthma

Eline Haspeslagh UGent, Nincy Debeuf UGent, Hamida Hammad UGent and Bart Lambrecht UGent (2017) Inflammation : methods and protocols. In Methods in Molecular Biology 1559. p.121-136
abstract
Allergic asthma is a heterogeneous inflammatory lung disease affecting millions of people worldwide and with a steadily increasing incidence. Mouse models have been of utmost importance in uncovering key inflammatory cell types, cytokines, and pathways in the development and maintenance of allergic asthma. Historically, the mainstay in experimental asthma research was sensitizing rodents to the model protein antigen ovalbumin (OVA) with the pro-Th2 adjuvant aluminum hydroxide, followed by repetitive OVA exposures to the airways to initiate a Th2-skewed adaptive immune response leading to eosinophilic airway inflammation and airway hyperreactivity (AHR). In the last 5 years, OVA is often replaced by naturally occurring allergens such as house dust mite (HDM) or cockroach extracts, but the principle of first sensitizing and then repetitively challenging mice with the same antigen is unchanged. Here, we describe an often used and relevant HDM-based protocol to establish acute allergic asthma, and the methods we have developed to rapidly analyze inflammatory cell infiltration in the bronchalveolar lavage fluid by flow cytometry. Moreover, we explain the methods to restimulate T cells from lung-draining mediastinal lymph nodes with HDM to allow the measurement of cytokine secretion profiles of allergen reactive T cells.
Please use this url to cite or link to this publication:
author
organization
year
type
bookChapter
publication status
published
subject
book title
Inflammation : methods and protocols
editor
Björn E Clausen and Jon D Laman
series title
Methods in Molecular Biology
volume
1559
pages
121 - 136
publisher
Springer
place of publication
New York, NY, USA
ISSN
1064-3745
ISBN
9781493967841
9781493967865
DOI
10.1007/978-1-4939-6786-5_10
language
English
UGent publication?
yes
classification
B2
copyright statement
I have transferred the copyright for this publication to the publisher
id
8511457
handle
http://hdl.handle.net/1854/LU-8511457
date created
2017-02-27 11:29:27
date last changed
2017-02-28 10:25:32
@incollection{8511457,
  abstract     = {Allergic asthma is a heterogeneous inflammatory lung disease affecting millions of people worldwide and with a steadily increasing incidence. Mouse models have been of utmost importance in uncovering key inflammatory cell types, cytokines, and pathways in the development and maintenance of allergic asthma. Historically, the mainstay in experimental asthma research was sensitizing rodents to the model protein antigen ovalbumin (OVA) with the pro-Th2 adjuvant aluminum hydroxide, followed by repetitive OVA exposures to the airways to initiate a Th2-skewed adaptive immune response leading to eosinophilic airway inflammation and airway hyperreactivity (AHR). In the last 5 years, OVA is often replaced by naturally occurring allergens such as house dust mite (HDM) or cockroach extracts, but the principle of first sensitizing and then repetitively challenging mice with the same antigen is unchanged. Here, we describe an often used and relevant HDM-based protocol to establish acute allergic asthma, and the methods we have developed to rapidly analyze inflammatory cell infiltration in the bronchalveolar lavage fluid by flow cytometry. Moreover, we explain the methods to restimulate T cells from lung-draining mediastinal lymph nodes with HDM to allow the measurement of cytokine secretion profiles of allergen reactive T cells.},
  author       = {Haspeslagh, Eline and Debeuf, Nincy and Hammad, Hamida and Lambrecht, Bart},
  booktitle    = {Inflammation : methods and protocols},
  editor       = {Clausen, Bj{\"o}rn E and Laman, Jon D},
  isbn         = {9781493967841},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {121--136},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Murine models of allergic asthma},
  url          = {http://dx.doi.org/10.1007/978-1-4939-6786-5\_10},
  volume       = {1559},
  year         = {2017},
}

Chicago
Haspeslagh, Eline, Nincy Debeuf, Hamida Hammad, and Bart Lambrecht. 2017. “Murine Models of Allergic Asthma.” In Inflammation : Methods and Protocols, ed. Björn E Clausen and Jon D Laman, 1559:121–136. New York, NY, USA: Springer.
APA
Haspeslagh, E., Debeuf, N., Hammad, H., & Lambrecht, B. (2017). Murine models of allergic asthma. In B. E. Clausen & J. D. Laman (Eds.), Inflammation : methods and protocols (Vol. 1559, pp. 121–136). New York, NY, USA: Springer.
Vancouver
1.
Haspeslagh E, Debeuf N, Hammad H, Lambrecht B. Murine models of allergic asthma. In: Clausen BE, Laman JD, editors. Inflammation : methods and protocols. New York, NY, USA: Springer; 2017. p. 121–36.
MLA
Haspeslagh, Eline, Nincy Debeuf, Hamida Hammad, et al. “Murine Models of Allergic Asthma.” Inflammation : Methods and Protocols. Ed. Björn E Clausen & Jon D Laman. Vol. 1559. New York, NY, USA: Springer, 2017. 121–136. Print.