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Effects of domestic chemical stressors on expression of allergen genes in the European house dust mite

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Abstract
The expression of allergen genes in house dust mites is influenced by temperature and relative humidity, but little is known of the impacts of other environmental factors that may alter the repertoire of allergens released by mites in home microhabitats. Bioassays were conducted in concave microscope slides in combination with real-time quantitative polymerase chain reaction (RT-qPCR) to analyse gene expression of 17 allergens of Dermatophagoides pteronyssinus (Acariformes: Pyroglyphidae) exposed to three chemical stressors that can be present in domestic environments. Short-term exposure (5–12 days) to diesel exhaust particles (DEPs) (1 µg/cm2), bacterial lipopolysaccharide endotoxin (0.1 µg/cm2) and benzyl benzoate (3.2 µg/cm2), at concentrations exceeding those expected in homes, had no significant effect on allergen transcription. A significant increase in the transcription of allergens Der p 3, Der p 8 and Der p 21 was observed only after exposing mites to a higher concentration of DEPs (10 µg/cm2) over a whole generation. In combination, the present results suggest that the analysed factors have low impact on allergen production. The methodology described here offers a sound and rapid approach to the broad-spectrum study of factors affecting allergen-related mite physiology, and allows the simultaneous screening of different factors in a relatively short period with consideration of the full spectrum of allergen genes.
Keywords
Acaricides, air pollution, allergy, bioassay, diesel exhaust particles, house dust mites, lipopolysaccharide, real-time quantitative PCR, EXPOSURE, POLLEN, ASTHMA

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MLA
Vidal-Quist, JC et al. “Effects of Domestic Chemical Stressors on Expression of Allergen Genes in the European House Dust Mite.” MEDICAL AND VETERINARY ENTOMOLOGY 31.1 (2017): 97–101. Print.
APA
Vidal-Quist, J., Ortego, F., Lambrecht, B., Castañera, P., & Hernández-Crespo, P. (2017). Effects of domestic chemical stressors on expression of allergen genes in the European house dust mite. MEDICAL AND VETERINARY ENTOMOLOGY, 31(1), 97–101.
Chicago author-date
Vidal-Quist, JC, F Ortego, Bart Lambrecht, P Castañera, and P Hernández-Crespo. 2017. “Effects of Domestic Chemical Stressors on Expression of Allergen Genes in the European House Dust Mite.” Medical and Veterinary Entomology 31 (1): 97–101.
Chicago author-date (all authors)
Vidal-Quist, JC, F Ortego, Bart Lambrecht, P Castañera, and P Hernández-Crespo. 2017. “Effects of Domestic Chemical Stressors on Expression of Allergen Genes in the European House Dust Mite.” Medical and Veterinary Entomology 31 (1): 97–101.
Vancouver
1.
Vidal-Quist J, Ortego F, Lambrecht B, Castañera P, Hernández-Crespo P. Effects of domestic chemical stressors on expression of allergen genes in the European house dust mite. MEDICAL AND VETERINARY ENTOMOLOGY. 2017;31(1):97–101.
IEEE
[1]
J. Vidal-Quist, F. Ortego, B. Lambrecht, P. Castañera, and P. Hernández-Crespo, “Effects of domestic chemical stressors on expression of allergen genes in the European house dust mite,” MEDICAL AND VETERINARY ENTOMOLOGY, vol. 31, no. 1, pp. 97–101, 2017.
@article{8511451,
  abstract     = {The expression of allergen genes in house dust mites is influenced by temperature and relative humidity, but little is known of the impacts of other environmental factors that may alter the repertoire of allergens released by mites in home microhabitats. Bioassays were conducted in concave microscope slides in combination with real-time quantitative polymerase chain reaction (RT-qPCR) to analyse gene expression of 17 allergens of Dermatophagoides pteronyssinus (Acariformes: Pyroglyphidae) exposed to three chemical stressors that can be present in domestic environments. Short-term exposure (5–12 days) to diesel exhaust particles (DEPs) (1 µg/cm2), bacterial lipopolysaccharide endotoxin (0.1 µg/cm2) and benzyl benzoate (3.2 µg/cm2), at concentrations exceeding those expected in homes, had no significant effect on allergen transcription. A significant increase in the transcription of allergens Der p 3, Der p 8 and Der p 21 was observed only after exposing mites to a higher concentration of DEPs (10 µg/cm2) over a whole generation. In combination, the present results suggest that the analysed factors have low impact on allergen production. The methodology described here offers a sound and rapid approach to the broad-spectrum study of factors affecting allergen-related mite physiology, and allows the simultaneous screening of different factors in a relatively short period with consideration of the full spectrum of allergen genes.},
  author       = {Vidal-Quist, JC and Ortego, F and Lambrecht, Bart and Castañera, P and Hernández-Crespo, P},
  issn         = {0269-283X},
  journal      = {MEDICAL AND VETERINARY ENTOMOLOGY},
  keywords     = {Acaricides,air pollution,allergy,bioassay,diesel exhaust particles,house dust mites,lipopolysaccharide,real-time quantitative PCR,EXPOSURE,POLLEN,ASTHMA},
  language     = {eng},
  number       = {1},
  pages        = {97--101},
  title        = {Effects of domestic chemical stressors on expression of allergen genes in the European house dust mite},
  url          = {http://dx.doi.org/10.1111/mve.12200},
  volume       = {31},
  year         = {2017},
}

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