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Mouse models of asthma

Nincy Debeuf (UGent) , Eline Haspeslagh (UGent) , Mary van Helden (UGent) , Hamida Hammad (UGent) and Bart Lambrecht (UGent)
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Abstract
Allergic asthma is a chronic inflammatory disease of the conducting airways characterized by the presence of allergen-specific IgE, Th2 cytokine production, eosinophilic airway inflammation, bronchial hyperreactivity, mucus overproduction, and structural changes in the airways. Investigators have tried to mimic these features of human allergic asthma in murine models. Whereas the surrogate allergen ovalbumin has been extremely valuable for unravelling underlying mechanisms of the disease, murine asthma models depend nowadays on naturally occurring allergens, such as house dust mite (HDM), cockroach, and Alternaria alternata. Here we describe a physiologically relevant model of acute allergic asthma based on sensitization and challenge with HDM extracts, and compare it with the ovalbumin/alum-induced asthma model. Moreover, we propose a detailed readout of the asthma phenotype, determining the degree of eosinophilia in bronchoalveolar lavage fluids by flow cytometry, visualizing goblet cell metaplasia, and measuring Th cytokine production by lung-draining mediastinal lymph node cells restimulated with HDM

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Chicago
Debeuf, Nincy, Eline Haspeslagh, Mary van Helden, Hamida Hammad, and Bart Lambrecht. 2016. “Mouse Models of Asthma.” In Current Protocols in Mouse Biology, ed. Johan Auwerx, Susan L Ackerman, Stephen D Brown, Yann Herault, Monica Justice, and Cheryl L Scudamore, 6:169–184. Hoboken, NJ, USA: Wiley.
APA
Debeuf, N., Haspeslagh, E., van Helden, M., Hammad, H., & Lambrecht, B. (2016). Mouse models of asthma. In J. Auwerx, S. L. Ackerman, S. D. Brown, Y. Herault, M. Justice, & C. L. Scudamore (Eds.), Current protocols in mouse biology (Vol. 6, pp. 169–184). Hoboken, NJ, USA: Wiley.
Vancouver
1.
Debeuf N, Haspeslagh E, van Helden M, Hammad H, Lambrecht B. Mouse models of asthma. In: Auwerx J, Ackerman SL, Brown SD, Herault Y, Justice M, Scudamore CL, editors. Current protocols in mouse biology. Hoboken, NJ, USA: Wiley; 2016. p. 169–84.
MLA
Debeuf, Nincy, Eline Haspeslagh, Mary van Helden, et al. “Mouse Models of Asthma.” Current Protocols in Mouse Biology. Ed. Johan Auwerx et al. Vol. 6. Hoboken, NJ, USA: Wiley, 2016. 169–184. Print.
@incollection{8511411,
  abstract     = {Allergic asthma is a chronic inflammatory disease of the conducting airways characterized by the presence of allergen-specific IgE, Th2 cytokine production, eosinophilic airway inflammation, bronchial hyperreactivity, mucus overproduction, and structural changes in the airways. Investigators have tried to mimic these features of human allergic asthma in murine models. Whereas the surrogate allergen ovalbumin has been extremely valuable for unravelling underlying mechanisms of the disease, murine asthma models depend nowadays on naturally occurring allergens, such as house dust mite (HDM), cockroach, and Alternaria alternata. Here we describe a physiologically relevant model of acute allergic asthma based on sensitization and challenge with HDM extracts, and compare it with the ovalbumin/alum-induced asthma model. Moreover, we propose a detailed readout of the asthma phenotype, determining the degree of eosinophilia in bronchoalveolar lavage fluids by flow cytometry, visualizing goblet cell metaplasia, and measuring Th cytokine production by lung-draining mediastinal lymph node cells restimulated with HDM},
  author       = {Debeuf, Nincy and Haspeslagh, Eline and van Helden, Mary and Hammad, Hamida and Lambrecht, Bart},
  booktitle    = {Current protocols in mouse biology},
  editor       = {Auwerx, Johan and Ackerman, Susan L and Brown, Stephen D and Herault, Yann and Justice, Monica and Scudamore, Cheryl L},
  isbn         = {9780470942390},
  language     = {eng},
  pages        = {169--184},
  publisher    = {Wiley},
  title        = {Mouse models of asthma},
  url          = {http://dx.doi.org/10.1002/cpmo.4},
  volume       = {6},
  year         = {2016},
}

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