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Silencing of two insulin receptor genes disrupts nymph-adult transition of alate brown citrus aphid

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Abstract
Insulin receptors play key roles in growth, development, and polymorphism in insects. Here, we report two insulin receptor genes (AcInR1 and AcInR2) from the brown citrus aphid, Aphis (Toxoptera) citricidus. Transcriptional analyses showed that AcInR1 increased during the nymph-adult transition in alate aphids, while AcInR2 had the highest expression level in second instar nymphs. AcInR1 is important in aphid development from fourth instar nymphs to adults as verified by dsRNA feeding mediated RNAi. The silencing of AcInR1 or/and AcInR2 produced a variety of phenotypes including adults with normal wings, malformed wings, under-developed wings, and aphids failing to develop beyond the nymphal stages. Silencing of AcInR1 or AcInR2 alone, and co-silencing of both genes, resulted in 73% or 60%, and 87% of aphids with problems in the transition from nymph to normal adult. The co-silencing of AcInR1 and AcInR2 resulted in 62% dead nymphs, but no mortality occurred by silencing of AcInR1 or AcInR2 alone. Phenotypes of adults in the dsInR1 and dsInR2 were similar. The results demonstrate that AcInR1 and AcInR2 are essential for successful nymph-adult transition in alate aphids and show that RNAi methods may be useful for the management of this pest.
Keywords
Aphis (Toxoptera) citricidus, insulin receptor genes, RNAi, development, TOXOPTERA-CITRICIDA, WING DEVELOPMENT, PEA APHID, DIFFERENTIAL EXPRESSION, TRIBOLIUM-CASTANEUM, SIGNALING PATHWAY, GROWTH-CONTROL, DROSOPHILA, PEPTIDES, MORPHS

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MLA
Ding, Bi-Yue et al. “Silencing of Two Insulin Receptor Genes Disrupts Nymph-adult Transition of Alate Brown Citrus Aphid.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.2 (2017): n. pag. Print.
APA
Ding, B.-Y., Shang, F., Zhang, Q., Xiong, Y., Yang, Q., Niu, J.-Z., Smagghe, G., et al. (2017). Silencing of two insulin receptor genes disrupts nymph-adult transition of alate brown citrus aphid. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 18(2).
Chicago author-date
Ding, Bi-Yue, Feng Shang, Qiang Zhang, Ying Xiong, Qun Yang, Jin-Zhi Niu, Guy Smagghe, and Jin-Jun Wang. 2017. “Silencing of Two Insulin Receptor Genes Disrupts Nymph-adult Transition of Alate Brown Citrus Aphid.” International Journal of Molecular Sciences 18 (2).
Chicago author-date (all authors)
Ding, Bi-Yue, Feng Shang, Qiang Zhang, Ying Xiong, Qun Yang, Jin-Zhi Niu, Guy Smagghe, and Jin-Jun Wang. 2017. “Silencing of Two Insulin Receptor Genes Disrupts Nymph-adult Transition of Alate Brown Citrus Aphid.” International Journal of Molecular Sciences 18 (2).
Vancouver
1.
Ding B-Y, Shang F, Zhang Q, Xiong Y, Yang Q, Niu J-Z, et al. Silencing of two insulin receptor genes disrupts nymph-adult transition of alate brown citrus aphid. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2017;18(2).
IEEE
[1]
B.-Y. Ding et al., “Silencing of two insulin receptor genes disrupts nymph-adult transition of alate brown citrus aphid,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 18, no. 2, 2017.
@article{8511409,
  abstract     = {{Insulin receptors play key roles in growth, development, and polymorphism in insects. Here, we report two insulin receptor genes (AcInR1 and AcInR2) from the brown citrus aphid, Aphis (Toxoptera) citricidus. Transcriptional analyses showed that AcInR1 increased during the nymph-adult transition in alate aphids, while AcInR2 had the highest expression level in second instar nymphs. AcInR1 is important in aphid development from fourth instar nymphs to adults as verified by dsRNA feeding mediated RNAi. The silencing of AcInR1 or/and AcInR2 produced a variety of phenotypes including adults with normal wings, malformed wings, under-developed wings, and aphids failing to develop beyond the nymphal stages. Silencing of AcInR1 or AcInR2 alone, and co-silencing of both genes, resulted in 73% or 60%, and 87% of aphids with problems in the transition from nymph to normal adult. The co-silencing of AcInR1 and AcInR2 resulted in 62% dead nymphs, but no mortality occurred by silencing of AcInR1 or AcInR2 alone. Phenotypes of adults in the dsInR1 and dsInR2 were similar. The results demonstrate that AcInR1 and AcInR2 are essential for successful nymph-adult transition in alate aphids and show that RNAi methods may be useful for the management of this pest.}},
  articleno    = {{357}},
  author       = {{Ding, Bi-Yue and Shang, Feng and Zhang, Qiang and Xiong, Ying and Yang, Qun and Niu, Jin-Zhi and Smagghe, Guy and Wang, Jin-Jun}},
  issn         = {{1422-0067}},
  journal      = {{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}},
  keywords     = {{Aphis (Toxoptera) citricidus,insulin receptor genes,RNAi,development,TOXOPTERA-CITRICIDA,WING DEVELOPMENT,PEA APHID,DIFFERENTIAL EXPRESSION,TRIBOLIUM-CASTANEUM,SIGNALING PATHWAY,GROWTH-CONTROL,DROSOPHILA,PEPTIDES,MORPHS}},
  language     = {{eng}},
  number       = {{2}},
  title        = {{Silencing of two insulin receptor genes disrupts nymph-adult transition of alate brown citrus aphid}},
  url          = {{http://dx.doi.org/10.3390/ijms18020357}},
  volume       = {{18}},
  year         = {{2017}},
}

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