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Isolation and identification of conventional dendritic cell subsets from the intestine of mice and men

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Abstract
The identification of conventional dendritic cells (cDCs) in the intestinal mucosa has been hampered by the difficulties associated with isolating cells from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from other intestinal mononuclear phagocytes such as macrophages (MFs). Here we detail the protocols we have developed to isolate live leukocytes from both murine and human small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs in such preparations.

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Citation

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Chicago
Scott, Charlotte, Pamela B Wright, Simon WF Milling, and Allan Mowat. 2016. “Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men.” In Dendritic Cell Protocols, ed. Elodie Segura and Nobuyuki Onai, 1423:101–118. New York, NY, USA: Springer.
APA
Scott, C., Wright, P. B., Milling, S. W., & Mowat, A. (2016). Isolation and identification of conventional dendritic cell subsets from the intestine of mice and men. In E. Segura & N. Onai (Eds.), Dendritic cell protocols (Vol. 1423, pp. 101–118). New York, NY, USA: Springer.
Vancouver
1.
Scott C, Wright PB, Milling SW, Mowat A. Isolation and identification of conventional dendritic cell subsets from the intestine of mice and men. In: Segura E, Onai N, editors. Dendritic cell protocols. New York, NY, USA: Springer; 2016. p. 101–18.
MLA
Scott, Charlotte et al. “Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men.” Dendritic Cell Protocols. Ed. Elodie Segura & Nobuyuki Onai. Vol. 1423. New York, NY, USA: Springer, 2016. 101–118. Print.
@incollection{8511405,
  abstract     = {The identification of conventional dendritic cells (cDCs) in the intestinal mucosa has been hampered by the difficulties associated with isolating cells from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from other intestinal mononuclear phagocytes such as macrophages (MFs). Here we detail the protocols we have developed to isolate live leukocytes from both murine and human small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs in such preparations.},
  author       = {Scott, Charlotte and Wright, Pamela B and Milling, Simon WF and Mowat, Allan},
  booktitle    = {Dendritic cell protocols},
  editor       = {Segura, Elodie and Onai, Nobuyuki},
  isbn         = {9781493936045},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {101--118},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Isolation and identification of conventional dendritic cell subsets from the intestine of mice and men},
  url          = {http://dx.doi.org/10.1007/978-1-4939-3606-9_7},
  volume       = {1423},
  year         = {2016},
}

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