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Performance of a TthPrimPol-based whole genome amplification kit for copy number alteration detection using massively parallel sequencing

Lieselot Deleye (UGent) , Dieter De Coninck (UGent) , Annelies Dheedene (UGent) , Petra De Sutter (UGent) , Björn Menten (UGent) , Dieter Deforce (UGent) and Filip Van Nieuwerburgh (UGent)
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Abstract
Starting from only a few cells, current whole genome amplification (WGA) methods provide enough DNA to perform massively parallel sequencing (MPS). Unfortunately, all current WGA methods introduce representation bias which limits detection of copy number aberrations (CNAs) smaller than 3 Mb. A recent WGA method, called TruePrime single cell WGA, uses a recently discovered DNA primase, TthPrimPol, instead of artificial primers to initiate DNA amplification. This method could lead to a lower representation bias, and consequently to a better detection of CNAs. The enzyme requires no complementarity and thus should generate random primers, equally distributed across the genome. The performance of TruePrime WGA was assessed for aneuploidy screening and CNA analysis after MPS, starting from 1, 3 or 5 cells. Although the method looks promising, the single cell TruePrime WGA kit v1 is not suited for high resolution CNA detection after MPS because too much representation bias is introduced.

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MLA
Deleye, Lieselot et al. “Performance of a TthPrimPol-based Whole Genome Amplification Kit for Copy Number Alteration Detection Using Massively Parallel Sequencing.” SCIENTIFIC REPORTS 6 (2016): n. pag. Print.
APA
Deleye, L., De Coninck, D., Dheedene, A., De Sutter, P., Menten, B., Deforce, D., & Van Nieuwerburgh, F. (2016). Performance of a TthPrimPol-based whole genome amplification kit for copy number alteration detection using massively parallel sequencing. SCIENTIFIC REPORTS, 6.
Chicago author-date
Deleye, Lieselot, Dieter De Coninck, Annelies Dheedene, Petra De Sutter, Björn Menten, Dieter Deforce, and Filip Van Nieuwerburgh. 2016. “Performance of a TthPrimPol-based Whole Genome Amplification Kit for Copy Number Alteration Detection Using Massively Parallel Sequencing.” Scientific Reports 6.
Chicago author-date (all authors)
Deleye, Lieselot, Dieter De Coninck, Annelies Dheedene, Petra De Sutter, Björn Menten, Dieter Deforce, and Filip Van Nieuwerburgh. 2016. “Performance of a TthPrimPol-based Whole Genome Amplification Kit for Copy Number Alteration Detection Using Massively Parallel Sequencing.” Scientific Reports 6.
Vancouver
1.
Deleye L, De Coninck D, Dheedene A, De Sutter P, Menten B, Deforce D, et al. Performance of a TthPrimPol-based whole genome amplification kit for copy number alteration detection using massively parallel sequencing. SCIENTIFIC REPORTS. 2016;6.
IEEE
[1]
L. Deleye et al., “Performance of a TthPrimPol-based whole genome amplification kit for copy number alteration detection using massively parallel sequencing,” SCIENTIFIC REPORTS, vol. 6, 2016.
@article{8511143,
  abstract     = {Starting from only a few cells, current whole genome amplification (WGA) methods provide enough DNA to perform massively parallel sequencing (MPS). Unfortunately, all current WGA methods introduce representation bias which limits detection of copy number aberrations (CNAs) smaller than 3 Mb. A recent WGA method, called TruePrime single cell WGA, uses a recently discovered DNA primase, TthPrimPol, instead of artificial primers to initiate DNA amplification. This method could lead to a lower representation bias, and consequently to a better detection of CNAs. The enzyme requires no complementarity and thus should generate random primers, equally distributed across the genome. The performance of TruePrime WGA was assessed for aneuploidy screening and CNA analysis after MPS, starting from 1, 3 or 5 cells. Although the method looks promising, the single cell TruePrime WGA kit v1 is not suited for high resolution CNA detection after MPS because too much representation bias is introduced.},
  articleno    = {31825},
  author       = {Deleye, Lieselot and De Coninck, Dieter and Dheedene, Annelies and De Sutter, Petra and Menten, Björn and Deforce, Dieter and Van Nieuwerburgh, Filip},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  language     = {eng},
  pages        = {9},
  title        = {Performance of a TthPrimPol-based whole genome amplification kit for copy number alteration detection using massively parallel sequencing},
  url          = {http://dx.doi.org/10.1038/srep31825},
  volume       = {6},
  year         = {2016},
}

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