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Aggregate size and architecture determine microbial activity balance for one-stage partial nitritation and anammox

Siegfried Vlaeminck UGent, Akihiko Terada, Barth F Smets, Haydée De Clippeleir UGent, Thomas Schaubroeck UGent, Selin Bolca UGent, Lien Demeestere, Jan Mast, Nico Boon UGent and Marta Carballa, et al. (2010) APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 76(3). p.900-909
abstract
Aerobic ammonium-oxidizing bacteria (AerAOB) and anoxic ammonium-oxidizing bacteria (AnAOB) cooperate in partial nitritation/anammox systems to remove ammonium from wastewater. In this process, large granular microbial aggregates enhance the performance, but little is known about granulation so far. In this study, three suspended-growth oxygen-limited autotrophic nitrification-denitrification (OLAND) reactors with different inoculation and operation (mixing and aeration) conditions, designated reactors A, B, and C, were used. The test objectives were (i) to quantify the AerAOB and AnAOB abundance and the activity balance for the different aggregate sizes and (ii) to relate aggregate morphology, size distribution, and architecture putatively to the inoculation and operation of the three reactors. A nitrite accumulation rate ratio (NARR) was defined as the net aerobic nitrite production rate divided by the anoxic nitrite consumption rate. The smallest reactor A, B, and C aggregates were nitrite sources (NARR, > 1.7). Large reactor A and C aggregates were granules capable of autonomous nitrogen removal (NARR, 0.6 to 1.1) with internal AnAOB zones surrounded by an AerAOB rim. Around 50% of the autotrophic space in these granules consisted of AerAOB- and AnAOB-specific extracellular polymeric substances. Large reactor B aggregates were thin film-like nitrite sinks (NARR, < 0.5) in which AnAOB were not shielded by an AerAOB layer. Voids and channels occupied 13 to 17% of the anoxic zone of AnAOB-rich aggregates (reactors B and C). The hypothesized granulation pathways include granule replication by division and budding and are driven by growth and/or decay based on species-specific physiology and by hydrodynamic shear and mixing.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
NITROGEN REMOVAL, AEROBIC GRANULE, EXTRACELLULAR POLYMERIC SUBSTANCES, GAS-LIFT REACTOR, BACTERIA, CANON, WATER, BIOFILM, SLUDGE, CELLS
journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Appl. Environ. Microbiol.
volume
76
issue
3
pages
900 - 909
Web of Science type
Article
Web of Science id
000274017400032
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.778 (2010)
JCR rank
32/158 (2010)
JCR quartile
1 (2010)
ISSN
0099-2240
DOI
10.1128/AEM.02337-09
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
851057
handle
http://hdl.handle.net/1854/LU-851057
date created
2010-02-03 21:57:32
date last changed
2010-02-26 13:45:07
@article{851057,
  abstract     = {Aerobic ammonium-oxidizing bacteria (AerAOB) and anoxic ammonium-oxidizing bacteria (AnAOB) cooperate in partial nitritation/anammox systems to remove ammonium from wastewater. In this process, large granular microbial aggregates enhance the performance, but little is known about granulation so far. In this study, three suspended-growth oxygen-limited autotrophic nitrification-denitrification (OLAND) reactors with different inoculation and operation (mixing and aeration) conditions, designated reactors A, B, and C, were used. The test objectives were (i) to quantify the AerAOB and AnAOB abundance and the activity balance for the different aggregate sizes and (ii) to relate aggregate morphology, size distribution, and architecture putatively to the inoculation and operation of the three reactors. A nitrite accumulation rate ratio (NARR) was defined as the net aerobic nitrite production rate divided by the anoxic nitrite consumption rate. The smallest reactor A, B, and C aggregates were nitrite sources (NARR, {\textrangle} 1.7). Large reactor A and C aggregates were granules capable of autonomous nitrogen removal (NARR, 0.6 to 1.1) with internal AnAOB zones surrounded by an AerAOB rim. Around 50\% of the autotrophic space in these granules consisted of AerAOB- and AnAOB-specific extracellular polymeric substances. Large reactor B aggregates were thin film-like nitrite sinks (NARR, {\textlangle} 0.5) in which AnAOB were not shielded by an AerAOB layer. Voids and channels occupied 13 to 17\% of the anoxic zone of AnAOB-rich aggregates (reactors B and C). The hypothesized granulation pathways include granule replication by division and budding and are driven by growth and/or decay based on species-specific physiology and by hydrodynamic shear and mixing.},
  author       = {Vlaeminck, Siegfried and Terada, Akihiko and Smets, Barth F and De Clippeleir, Hayd{\'e}e and Schaubroeck, Thomas and Bolca, Selin and Demeestere, Lien and Mast, Jan and Boon, Nico and Carballa, Marta and Verstraete, Willy},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {NITROGEN REMOVAL,AEROBIC GRANULE,EXTRACELLULAR POLYMERIC SUBSTANCES,GAS-LIFT REACTOR,BACTERIA,CANON,WATER,BIOFILM,SLUDGE,CELLS},
  language     = {eng},
  number       = {3},
  pages        = {900--909},
  title        = {Aggregate size and architecture determine microbial activity balance for one-stage partial nitritation and anammox},
  url          = {http://dx.doi.org/10.1128/AEM.02337-09},
  volume       = {76},
  year         = {2010},
}

Chicago
Vlaeminck, Siegfried, Akihiko Terada, Barth F Smets, Haydée De Clippeleir, Thomas Schaubroeck, Selin Bolca, Lien Demeestere, et al. 2010. “Aggregate Size and Architecture Determine Microbial Activity Balance for One-stage Partial Nitritation and Anammox.” Applied and Environmental Microbiology 76 (3): 900–909.
APA
Vlaeminck, S., Terada, A., Smets, B. F., De Clippeleir, H., Schaubroeck, T., Bolca, S., Demeestere, L., et al. (2010). Aggregate size and architecture determine microbial activity balance for one-stage partial nitritation and anammox. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(3), 900–909.
Vancouver
1.
Vlaeminck S, Terada A, Smets BF, De Clippeleir H, Schaubroeck T, Bolca S, et al. Aggregate size and architecture determine microbial activity balance for one-stage partial nitritation and anammox. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2010;76(3):900–9.
MLA
Vlaeminck, Siegfried, Akihiko Terada, Barth F Smets, et al. “Aggregate Size and Architecture Determine Microbial Activity Balance for One-stage Partial Nitritation and Anammox.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 76.3 (2010): 900–909. Print.