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RBOH-mediated ROS production facilitates lateral root emergence in Arabidopsis

(2016) DEVELOPMENT. 143(18). p.3328-3339
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Lateral root (LR) emergence represents a highly coordinated process in which the plant hormone auxin plays a central role. Reactive oxygen species (ROS) have been proposed to function as important signals during auxin-regulated LR formation, however their mode of action is poorly understood. Here, we report that Arabidopsis roots exposed to ROS show increased LR numbers due to the activation of LR pre-branch sites and LR primordia (LRP). Strikingly, ROS treatment can also restore LR formation in pCASP1:shy2-2 and aux1 lax3 mutant lines in which auxin-mediated cell wall accommodation and remodeling in cells overlying the sites of LR formation is disrupted. Specifically, ROS are deposited in the apoplast of these cells during LR emergence, following a spatio-temporal pattern that overlaps the combined expression domains of extracellular ROS donors of the RESPIRATORY BURST OXIDASE HOMOLOGS (RBOH). We also show that disrupting (or enhancing) expression of RBOH in LRP and/or overlying root tissues decelerates (or accelerates) the development and emergence of LRs. We conclude that RBOH-mediated ROS production facilitates LR outgrowth by promoting cell wall remodeling of overlying parental tissues.
Keywords
Auxin, Auxin-mediated cell wall remodeling, Lateral root emergence, Reactive oxygen species, Respiratory burst oxidase homologs, OF-FUNCTION MUTATION, REACTIVE OXYGEN, ASCORBATE PEROXIDASE, CELL-SEPARATION, AUXIN, INITIATION, GROWTH, THALIANA, H2O2, STRESS

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Citation

Please use this url to cite or link to this publication:

Chicago
Orman-Ligeza, Beata, Boris Parizot, Riet De Rycke, Ana Fernandez Salina, Ellie Himschoot, Frank Van Breusegem, Malcolm J Bennett, Claire Périlleux, Tom Beeckman, and Xavier Draye. 2016. “RBOH-mediated ROS Production Facilitates Lateral Root Emergence in Arabidopsis.” Development 143 (18): 3328–3339.
APA
Orman-Ligeza, B., Parizot, B., De Rycke, R., Fernandez Salina, A., Himschoot, E., Van Breusegem, F., Bennett, M. J., et al. (2016). RBOH-mediated ROS production facilitates lateral root emergence in Arabidopsis. DEVELOPMENT, 143(18), 3328–3339.
Vancouver
1.
Orman-Ligeza B, Parizot B, De Rycke R, Fernandez Salina A, Himschoot E, Van Breusegem F, et al. RBOH-mediated ROS production facilitates lateral root emergence in Arabidopsis. DEVELOPMENT. 2016;143(18):3328–39.
MLA
Orman-Ligeza, Beata, Boris Parizot, Riet De Rycke, et al. “RBOH-mediated ROS Production Facilitates Lateral Root Emergence in Arabidopsis.” DEVELOPMENT 143.18 (2016): 3328–3339. Print.
@article{8510563,
  abstract     = {Lateral root (LR) emergence represents a highly coordinated process in which the plant hormone auxin plays a central role. Reactive oxygen species (ROS) have been proposed to function as important signals during auxin-regulated LR formation, however their mode of action is poorly understood. Here, we report that Arabidopsis roots exposed to ROS show increased LR numbers due to the activation of LR pre-branch sites and LR primordia (LRP). Strikingly, ROS treatment can also restore LR formation in pCASP1:shy2-2 and aux1 lax3 mutant lines in which auxin-mediated cell wall accommodation and remodeling in cells overlying the sites of LR formation is disrupted. Specifically, ROS are deposited in the apoplast of these cells during LR emergence, following a spatio-temporal pattern that overlaps the combined expression domains of extracellular ROS donors of the RESPIRATORY BURST OXIDASE HOMOLOGS (RBOH). We also show that disrupting (or enhancing) expression of RBOH in LRP and/or overlying root tissues decelerates (or accelerates) the development and emergence of LRs. We conclude that RBOH-mediated ROS production facilitates LR outgrowth by promoting cell wall remodeling of overlying parental tissues.},
  author       = {Orman-Ligeza, Beata and Parizot, Boris and De Rycke, Riet and Fernandez Salina, Ana and Himschoot, Ellie and Van Breusegem, Frank and Bennett, Malcolm J and Périlleux, Claire and Beeckman, Tom and Draye, Xavier},
  issn         = {0950-1991},
  journal      = {DEVELOPMENT},
  keywords     = {Auxin,Auxin-mediated cell wall remodeling,Lateral root emergence,Reactive oxygen species,Respiratory burst oxidase homologs,OF-FUNCTION MUTATION,REACTIVE OXYGEN,ASCORBATE PEROXIDASE,CELL-SEPARATION,AUXIN,INITIATION,GROWTH,THALIANA,H2O2,STRESS},
  language     = {eng},
  number       = {18},
  pages        = {3328--3339},
  title        = {RBOH-mediated ROS production facilitates lateral root emergence in Arabidopsis},
  url          = {http://dx.doi.org/10.1242/dev.136465},
  volume       = {143},
  year         = {2016},
}

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