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Untargeted metabolomics of colonic digests reveals kynurenine pathway metabolites, dityrosine and 3-dehydroxycarnitine as red versus white meat discriminating metabolites

Caroline Rombouts (UGent) , Lieselot Hemeryck (UGent) , Thomas Van Hecke (UGent) , Stefaan De Smet (UGent) , Winnok De Vos (UGent) and Lynn Vanhaecke (UGent)
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Abstract
Epidemiological research has demonstrated that the consumption of red meat is an important risk factor for the development of colorectal cancer (CRC), diabetes mellitus and cardiovascular diseases. However, there is no holistic insight in the (by-) products of meat digestion that may contribute to disease development. To address this hiatus, an untargeted mass spectrometry (MS)-based metabolomics approach was used to create red versus white meat associated metabolic fingerprints following in vitro colonic digestion using the fecal inocula of ten healthy volunteers. Twenty-two metabolites were unequivocally associated with simulated colonic digestion of red meat. Several of these metabolites could mechanistically be linked to red meat-associated pathways including N’-formylkynurenine, kynurenine and kynurenic acid (all involved in tryptophan metabolism), the oxidative stress marker dityrosine, and 3-dehydroxycarnitine. In conclusion, the used MS-based metabolomics platform proved to be a powerful platform for detection of specific metabolites that improve the understanding of the causal relationship between red meat consumption and associated diseases.
Keywords
Red meat, Metabolomics, Mass spectrometry, Colorectal cancer, In vitro digestion, HUMAN GUT MICROBIOTA, IN-VITRO DIGESTION, COLORECTAL-CANCER, INDOLEAMINE 2, 3-DIOXYGENASE, L-CARNITINE, OXIDATION, CELLS, INHIBITION, PROTEIN, ACID

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Chicago
Rombouts, Caroline, Lieselot Hemeryck, Thomas Van Hecke, Stefaan De Smet, Winnok De Vos, and Lynn Vanhaecke. 2017. “Untargeted Metabolomics of Colonic Digests Reveals Kynurenine Pathway Metabolites, Dityrosine and 3-dehydroxycarnitine as Red Versus White Meat Discriminating Metabolites.” Scientific Reports 7.
APA
Rombouts, Caroline, Hemeryck, L., Van Hecke, T., De Smet, S., De Vos, W., & Vanhaecke, L. (2017). Untargeted metabolomics of colonic digests reveals kynurenine pathway metabolites, dityrosine and 3-dehydroxycarnitine as red versus white meat discriminating metabolites. SCIENTIFIC REPORTS, 7.
Vancouver
1.
Rombouts C, Hemeryck L, Van Hecke T, De Smet S, De Vos W, Vanhaecke L. Untargeted metabolomics of colonic digests reveals kynurenine pathway metabolites, dityrosine and 3-dehydroxycarnitine as red versus white meat discriminating metabolites. SCIENTIFIC REPORTS. 2017;7.
MLA
Rombouts, Caroline, Lieselot Hemeryck, Thomas Van Hecke, et al. “Untargeted Metabolomics of Colonic Digests Reveals Kynurenine Pathway Metabolites, Dityrosine and 3-dehydroxycarnitine as Red Versus White Meat Discriminating Metabolites.” SCIENTIFIC REPORTS 7 (2017): n. pag. Print.
@article{8510558,
  abstract     = {Epidemiological research has demonstrated that the consumption of red meat is an important risk factor for the development of colorectal cancer (CRC), diabetes mellitus and cardiovascular diseases. However, there is no holistic insight in the (by-) products of meat digestion that may contribute to disease development. To address this hiatus, an untargeted mass spectrometry (MS)-based metabolomics approach was used to create red versus white meat associated metabolic fingerprints following in vitro colonic digestion using the fecal inocula of ten healthy volunteers. Twenty-two metabolites were unequivocally associated with simulated colonic digestion of red meat. Several of these metabolites could mechanistically be linked to red meat-associated pathways including N{\textquoteright}-formylkynurenine, kynurenine and kynurenic acid (all involved in tryptophan metabolism), the oxidative stress marker dityrosine, and 3-dehydroxycarnitine. In conclusion, the used MS-based metabolomics platform proved to be a powerful platform for detection of specific metabolites that improve the understanding of the causal relationship between red meat consumption and associated diseases. },
  articleno    = {42514},
  author       = {Rombouts, Caroline and Hemeryck, Lieselot and Van Hecke, Thomas and De Smet, Stefaan and De Vos, Winnok and Vanhaecke, Lynn},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keyword      = {Red meat,Metabolomics,Mass spectrometry,Colorectal cancer,In vitro digestion,HUMAN GUT MICROBIOTA,IN-VITRO DIGESTION,COLORECTAL-CANCER,INDOLEAMINE 2,3-DIOXYGENASE,L-CARNITINE,OXIDATION,CELLS,INHIBITION,PROTEIN,ACID},
  language     = {eng},
  pages        = {13},
  title        = {Untargeted metabolomics of colonic digests reveals kynurenine pathway metabolites, dityrosine and 3-dehydroxycarnitine as red versus white meat discriminating metabolites},
  url          = {http://dx.doi.org/10.1038/srep42514},
  volume       = {7},
  year         = {2017},
}

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