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Cytokinin response factor 6 represses cytokinin-associated genes during oxidative stress

(2016) PLANT PHYSIOLOGY. 172(2). p.1249-1258
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Abstract
Cytokinin is a phytohormone that is well known for its roles in numerous plant growth and developmental processes, yet it has also been linked to abiotic stress response in a less defined manner. Arabidopsis (Arabidopsis thaliana) Cytokinin Response Factor 6 (CRF6) is a cytokinin-responsive AP2/ERF-family transcription factor that, through the cytokinin signaling pathway, plays a key role in the inhibition of dark-induced senescence. CRF6 expression is also induced by oxidative stress, and here we show a novel function for CRF6 in relation to oxidative stress and identify downstream transcriptional targets of CRF6 that are repressed in response to oxidative stress. Analysis of transcriptomic changes in wild-type and crf6 mutant plants treated with H2O2 identified CRF6-dependent differentially expressed transcripts, many of which were repressed rather than induced. Moreover, many repressed genes also show decreased expression in 35S:CRF6 overexpressing plants. Together, these findings suggest that CRF6 functions largely as a transcriptional repressor. Interestingly, among the H2O2 repressed CRF6-dependent transcripts was a set of five genes associated with cytokinin processes: (signaling) ARR6, ARR9, ARR11, (biosynthesis) LOG7, and (transport) ABCG14. We have examined mutants of these cytokinin-associated target genes to reveal novel connections to oxidative stress. Further examination of CRF6-DNA interactions indicated that CRF6 may regulate its targets both directly and indirectly. Together, this shows that CRF6 functions during oxidative stress as a negative regulator to control this cytokinin-associated module of CRF6-dependent genes and establishes a novel connection between cytokinin and oxidative stress response.
Keywords
NAC TRANSCRIPTION FACTOR, CHLOROPHYLL FLUORESCENCE, REGULATORS ARR1, ABSCISIC-ACID, B RESPONSE, ARABIDOPSIS, DROUGHT, TOLERANCE, PATHWAY, PERCEPTION

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MLA
Zwack, Paul J., et al. “Cytokinin Response Factor 6 Represses Cytokinin-Associated Genes during Oxidative Stress.” PLANT PHYSIOLOGY, vol. 172, no. 2, 2016, pp. 1249–58, doi:10.1104/pp.16.00415.
APA
Zwack, P. J., De Clercq, I., Howton, T. C., Hallmark, H. T., Hurny, A., Keshishian, E. A., … Rashotte, A. M. (2016). Cytokinin response factor 6 represses cytokinin-associated genes during oxidative stress. PLANT PHYSIOLOGY, 172(2), 1249–1258. https://doi.org/10.1104/pp.16.00415
Chicago author-date
Zwack, Paul J, Inge De Clercq, Timothy C Howton, H Tucker Hallmark, Andrej Hurny, Erika A Keshishian, Alyssa M Parish, et al. 2016. “Cytokinin Response Factor 6 Represses Cytokinin-Associated Genes during Oxidative Stress.” PLANT PHYSIOLOGY 172 (2): 1249–58. https://doi.org/10.1104/pp.16.00415.
Chicago author-date (all authors)
Zwack, Paul J, Inge De Clercq, Timothy C Howton, H Tucker Hallmark, Andrej Hurny, Erika A Keshishian, Alyssa M Parish, Eva Benkova, M Shahid Mukhtar, Frank Van Breusegem, and Aaron M Rashotte. 2016. “Cytokinin Response Factor 6 Represses Cytokinin-Associated Genes during Oxidative Stress.” PLANT PHYSIOLOGY 172 (2): 1249–1258. doi:10.1104/pp.16.00415.
Vancouver
1.
Zwack PJ, De Clercq I, Howton TC, Hallmark HT, Hurny A, Keshishian EA, et al. Cytokinin response factor 6 represses cytokinin-associated genes during oxidative stress. PLANT PHYSIOLOGY. 2016;172(2):1249–58.
IEEE
[1]
P. J. Zwack et al., “Cytokinin response factor 6 represses cytokinin-associated genes during oxidative stress,” PLANT PHYSIOLOGY, vol. 172, no. 2, pp. 1249–1258, 2016.
@article{8510547,
  abstract     = {{Cytokinin is a phytohormone that is well known for its roles in numerous plant growth and developmental processes, yet it has also been linked to abiotic stress response in a less defined manner. Arabidopsis (Arabidopsis thaliana) Cytokinin Response Factor 6 (CRF6) is a cytokinin-responsive AP2/ERF-family transcription factor that, through the cytokinin signaling pathway, plays a key role in the inhibition of dark-induced senescence. CRF6 expression is also induced by oxidative stress, and here we show a novel function for CRF6 in relation to oxidative stress and identify downstream transcriptional targets of CRF6 that are repressed in response to oxidative stress. Analysis of transcriptomic changes in wild-type and crf6 mutant plants treated with H2O2 identified CRF6-dependent differentially expressed transcripts, many of which were repressed rather than induced. Moreover, many repressed genes also show decreased expression in 35S:CRF6 overexpressing plants. Together, these findings suggest that CRF6 functions largely as a transcriptional repressor. Interestingly, among the H2O2 repressed CRF6-dependent transcripts was a set of five genes associated with cytokinin processes: (signaling) ARR6, ARR9, ARR11, (biosynthesis) LOG7, and (transport) ABCG14. We have examined mutants of these cytokinin-associated target genes to reveal novel connections to oxidative stress. Further examination of CRF6-DNA interactions indicated that CRF6 may regulate its targets both directly and indirectly. Together, this shows that CRF6 functions during oxidative stress as a negative regulator to control this cytokinin-associated module of CRF6-dependent genes and establishes a novel connection between cytokinin and oxidative stress response.}},
  author       = {{Zwack, Paul J and De Clercq, Inge and Howton, Timothy C and Hallmark, H Tucker and Hurny, Andrej and Keshishian, Erika A and Parish, Alyssa M and Benkova, Eva and Mukhtar, M Shahid and Van Breusegem, Frank and Rashotte, Aaron M}},
  issn         = {{0032-0889}},
  journal      = {{PLANT PHYSIOLOGY}},
  keywords     = {{NAC TRANSCRIPTION FACTOR,CHLOROPHYLL FLUORESCENCE,REGULATORS ARR1,ABSCISIC-ACID,B RESPONSE,ARABIDOPSIS,DROUGHT,TOLERANCE,PATHWAY,PERCEPTION}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{1249--1258}},
  title        = {{Cytokinin response factor 6 represses cytokinin-associated genes during oxidative stress}},
  url          = {{http://doi.org/10.1104/pp.16.00415}},
  volume       = {{172}},
  year         = {{2016}},
}

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