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Fermentation assays reveal differences in sugar and (off-) flavor metabolism across different Brettanomyces bruxellensis strains

Sam Crauwels, Filip Van Opstaele, Barbara Jaskula-Goiris, Jan Steensels, Christel Verreth, Lien Bosmans, Caroline Paulussen, Beatriz Herrera-Malaver, Ronnie de Jonge UGent, Jessika De Clippeleer, et al. (2017) FEMS YEAST RESEARCH. 17(1).
abstract
Brettanomyces (Dekkera) bruxellensis is an ascomycetous yeast of major importance in the food, beverage and biofuel industry. It has been isolated from various man-made ecological niches that are typically characterized by harsh environmental conditions such as wine, beer, soft drink, etc. Recent comparative genomics studies revealed an immense intraspecific diversity, but it is still unclear whether this genetic diversity also leads to systematic differences in fermentation performance and (off-)flavor production, and to what extent strains have evolved to match their ecological niche. Here, we present an evaluation of the fermentation properties of eight genetically diverse B. bruxellensis strains originating from beer, wine and soft drinks. We show that sugar consumption and aroma production during fermentation are determined by both the yeast strain and composition of the medium. Furthermore, our results indicate a strong niche adaptation of B. bruxellensis, most clearly for wine strains. For example, only strains originally isolated from wine were able to thrive well and produce the typical Brettanomyces-related phenolic off-flavors 4-ethylguaiacol and 4-ethylphenol when inoculated in red wine. Sulfite tolerance was found as a key factor explaining the observed differences in fermentation performance and off-flavor production. Sequence analysis of genes related to phenolic off-flavor production, however, revealed only marginal differences between the isolates tested, especially at the amino acid level. Altogether, our study provides novel insights in the Brettanomyces metabolism of flavor production, and is highly relevant for both the wine and beer industry.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
IBCN, (Off-)flavors, ecological niche, phenolic acid decarboxylase, vinylphenol reductase, volatile phenols
journal title
FEMS YEAST RESEARCH
FEMS Yeast Res.
volume
17
issue
1
article number
fow105
pages
10 pages
Web of Science type
Article
Web of Science id
000405634600009
ISSN
1567-1356
1567-1364
DOI
10.1093/femsyr/fow105
project
Bioinformatics: from nucleotids to networks (N2N)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8510459
handle
http://hdl.handle.net/1854/LU-8510459
date created
2017-02-20 18:32:31
date last changed
2017-09-12 13:06:54
@article{8510459,
  abstract     = {Brettanomyces (Dekkera) bruxellensis is an ascomycetous yeast of major importance in the food, beverage and biofuel industry. It has been isolated from various man-made ecological niches that are typically characterized by harsh environmental conditions such as wine, beer, soft drink, etc. Recent comparative genomics studies revealed an immense intraspecific diversity, but it is still unclear whether this genetic diversity also leads to systematic differences in fermentation performance and (off-)flavor production, and to what extent strains have evolved to match their ecological niche. Here, we present an evaluation of the fermentation properties of eight genetically diverse B. bruxellensis strains originating from beer, wine and soft drinks. We show that sugar consumption and aroma production during fermentation are determined by both the yeast strain and composition of the medium. Furthermore, our results indicate a strong niche adaptation of B. bruxellensis, most clearly for wine strains. For example, only strains originally isolated from wine were able to thrive well and produce the typical Brettanomyces-related phenolic off-flavors 4-ethylguaiacol and 4-ethylphenol when inoculated in red wine. Sulfite tolerance was found as a key factor explaining the observed differences in fermentation performance and off-flavor production. Sequence analysis of genes related to phenolic off-flavor production, however, revealed only marginal differences between the isolates tested, especially at the amino acid level. Altogether, our study provides novel insights in the Brettanomyces metabolism of flavor production, and is highly relevant for both the wine and beer industry.},
  articleno    = {fow105},
  author       = {Crauwels, Sam and Van Opstaele, Filip and Jaskula-Goiris, Barbara and Steensels, Jan and Verreth, Christel and Bosmans, Lien and Paulussen, Caroline and Herrera-Malaver, Beatriz and de Jonge, Ronnie and De Clippeleer, Jessika and Marchal, Kathleen and De Samblanx, Gorik and Willems, Kris A and Verstrepen, Kevin J and Aerts, Guido and Lievens, Bart},
  issn         = {1567-1356},
  journal      = {FEMS YEAST RESEARCH},
  keyword      = {IBCN,(Off-)flavors,ecological niche,phenolic acid decarboxylase,vinylphenol reductase,volatile phenols},
  language     = {eng},
  number       = {1},
  pages        = {10},
  title        = {Fermentation assays reveal differences in sugar and (off-) flavor metabolism across different Brettanomyces bruxellensis strains},
  url          = {http://dx.doi.org/10.1093/femsyr/fow105},
  volume       = {17},
  year         = {2017},
}

Chicago
Crauwels, Sam, Filip Van Opstaele, Barbara Jaskula-Goiris, Jan Steensels, Christel Verreth, Lien Bosmans, Caroline Paulussen, et al. 2017. “Fermentation Assays Reveal Differences in Sugar and (off-) Flavor Metabolism Across Different Brettanomyces Bruxellensis Strains.” Fems Yeast Research 17 (1).
APA
Crauwels, Sam, Van Opstaele, F., Jaskula-Goiris, B., Steensels, J., Verreth, C., Bosmans, L., Paulussen, C., et al. (2017). Fermentation assays reveal differences in sugar and (off-) flavor metabolism across different Brettanomyces bruxellensis strains. FEMS YEAST RESEARCH, 17(1).
Vancouver
1.
Crauwels S, Van Opstaele F, Jaskula-Goiris B, Steensels J, Verreth C, Bosmans L, et al. Fermentation assays reveal differences in sugar and (off-) flavor metabolism across different Brettanomyces bruxellensis strains. FEMS YEAST RESEARCH. 2017;17(1).
MLA
Crauwels, Sam, Filip Van Opstaele, Barbara Jaskula-Goiris, et al. “Fermentation Assays Reveal Differences in Sugar and (off-) Flavor Metabolism Across Different Brettanomyces Bruxellensis Strains.” FEMS YEAST RESEARCH 17.1 (2017): n. pag. Print.