Ghent University Academic Bibliography

Advanced

Expression of key components of the RNAi machinery are suppressed in Apis mellifera that suffer a high virus infection

Lina De Smet UGent, Jorgen Ravoet, Tom Wenseleers and Dirk de Graaf UGent (2017) ENTOMOLOGICAL SCIENCE. 20(1). p.76-85
abstract
Viruses are one of the major threats for honeybees and until now more than 20 different viruses have been discovered. Viruses and their hosts are engaged in a continuous arms race in which viral defense mechanisms drive the adaptive evolution of host immune genes, which in turn results in counter-adaptations of the viral immune antagonists. The honeybee immune responses to non-viral pathogens have been extensively studied, but little is known about the antiviral responses. Recent evidence suggests that the main mechanism of antiviral defense in insects is the RNA interference (RNAi) pathway. Furthermore, there is evidence that some viruses suppress this RNAi pathway in order to evade antiviral immunity. In the present study, we test this hypothesis by comparing the gene expression levels of some key components of the RNAi response of honeybees that were naturally infected with at least five viruses with those who were infected with only two or three viruses using a colorimetric microarray developed in-house, called BeeClinic, and subsequently confirmed by quantitative reverse-transcription-polymerase chain reaction (qRT-PCR). Our results show that key components of the RNAi pathway are indeed downregulated in highly infected bees. We were able to show that high virus loads suppress key RNAi components, which results in a counteraction of the host RNAi antiviral defense. As the RNAi is a primary defense against viruses, these findings shed new light on pathogen-host interactions and can help mitigate escalating colony losses worldwide.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
antiviral, BeeClinic, colorimetric microarray, Dicer, gene expression, honeybee, COLONY COLLAPSE DISORDER, HONEY-BEE VIRUSES, ANTIVIRAL IMMUNITY, INNATE IMMUNE, PCR DATA, DROSOPHILA, DEFENSE, INTERFERENCE, REPLICATION, INGESTION
journal title
ENTOMOLOGICAL SCIENCE
Entomol. Sci.
volume
20
issue
1
pages
76 - 85
Web of Science type
Article
Web of Science id
000396406800010
ISSN
1343-8786
DOI
10.1111/ens.12227
language
English
UGent publication?
yes
classification
A1
additional info
the first two authors contributed equally to this work
copyright statement
I have transferred the copyright for this publication to the publisher
id
8509582
handle
http://hdl.handle.net/1854/LU-8509582
date created
2017-02-15 10:56:51
date last changed
2017-05-10 12:32:52
@article{8509582,
  abstract     = {Viruses are one of the major threats for honeybees and until now more than 20 different viruses have been discovered. Viruses and their hosts are engaged in a continuous arms race in which viral defense mechanisms drive the adaptive evolution of host immune genes, which in turn results in counter-adaptations of the viral immune antagonists. The honeybee immune responses to non-viral pathogens have been extensively studied, but little is known about the antiviral responses. Recent evidence suggests that the main mechanism of antiviral defense in insects is the RNA interference (RNAi) pathway. Furthermore, there is evidence that some viruses suppress this RNAi pathway in order to evade antiviral immunity. In the present study, we test this hypothesis by comparing the gene expression levels of some key components of the RNAi response of honeybees that were naturally infected with at least five viruses with those who were infected with only two or three viruses using a colorimetric microarray developed in-house, called BeeClinic, and subsequently confirmed by quantitative reverse-transcription-polymerase chain reaction (qRT-PCR). Our results show that key components of the RNAi pathway are indeed downregulated in highly infected bees. We were able to show that high virus loads suppress key RNAi components, which results in a counteraction of the host RNAi antiviral defense. As the RNAi is a primary defense against viruses, these findings shed new light on pathogen-host interactions and can help mitigate escalating colony losses worldwide.},
  author       = {De Smet, Lina and Ravoet, Jorgen and Wenseleers, Tom and de Graaf, Dirk},
  issn         = {1343-8786},
  journal      = {ENTOMOLOGICAL SCIENCE},
  keyword      = {antiviral,BeeClinic,colorimetric microarray,Dicer,gene expression,honeybee,COLONY COLLAPSE DISORDER,HONEY-BEE VIRUSES,ANTIVIRAL IMMUNITY,INNATE IMMUNE,PCR DATA,DROSOPHILA,DEFENSE,INTERFERENCE,REPLICATION,INGESTION},
  language     = {eng},
  number       = {1},
  pages        = {76--85},
  title        = {Expression of key components of the RNAi machinery are suppressed in Apis mellifera that suffer a high virus infection},
  url          = {http://dx.doi.org/10.1111/ens.12227},
  volume       = {20},
  year         = {2017},
}

Chicago
De Smet, Lina, Jorgen Ravoet, Tom Wenseleers, and Dirk de Graaf. 2017. “Expression of Key Components of the RNAi Machinery Are Suppressed in Apis Mellifera That Suffer a High Virus Infection.” Entomological Science 20 (1): 76–85.
APA
De Smet, Lina, Ravoet, J., Wenseleers, T., & de Graaf, D. (2017). Expression of key components of the RNAi machinery are suppressed in Apis mellifera that suffer a high virus infection. ENTOMOLOGICAL SCIENCE, 20(1), 76–85.
Vancouver
1.
De Smet L, Ravoet J, Wenseleers T, de Graaf D. Expression of key components of the RNAi machinery are suppressed in Apis mellifera that suffer a high virus infection. ENTOMOLOGICAL SCIENCE. 2017;20(1):76–85.
MLA
De Smet, Lina, Jorgen Ravoet, Tom Wenseleers, et al. “Expression of Key Components of the RNAi Machinery Are Suppressed in Apis Mellifera That Suffer a High Virus Infection.” ENTOMOLOGICAL SCIENCE 20.1 (2017): 76–85. Print.