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Repetitive urine and blood sampling in neonatal and weaned piglets for pharmacokinetic and pharmacodynamic modelling in drug discovery : a pilot study

Elke Gasthuys UGent, Stijn Schauvliege UGent, Thomas van Bergen UGent, Joske Millecam UGent, Ilaria Cerasoli, Ann Martens UGent, Frank Gasthuys UGent, Tim Vandecasteele UGent, Pieter Cornillie UGent, Wim Van Den Broeck UGent, et al. (2017) LABORATORY ANIMALS.
abstract
Piglets are considered to be suitable animal models for predicting the pharmacokinetics and pharmacodynamics (PK/PD) of test drugs for potential use in the paediatric population. Such PK/PD studies require multiple blood and urine samplings. The goal of the present study was to determine a suitable blood collection strategy applicable in the youngest age categories of six days, four weeks and eight weeks of age, as well as a urine collection technique for male piglets in the same age categories. Blood was collected either by a surgically-placed jugular vein catheter (six days old [n = 4] and four weeks old [n = 2] piglets) or by direct venepuncture of the jugular vein (four weeks old [n = 2] and eight weeks old [n = 4] piglets). A non-invasive method for total volume urine collection in male piglets was also developed using a urine pouch. No specific complications were encountered during anaesthesia or surgery for jugular catheter placement. After a 24 h recovery period, urine and blood were easily collected without technical complications. One piglet was humanely killed at week 2 because of septicaemia. Histological analysis of both veins in all four piglets revealed negligible damage to the blood vessel wall. In conclusion, the presented techniques for blood (jugular catheter and direct venepuncture) and urine collection (pouches) are suitable for PK/PD studies in piglets.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
in press
subject
keyword
blood sampling, intravenous catheterization, neonate, piglet, urine collection
journal title
LABORATORY ANIMALS
Lab. Anim.
ISSN
0023-6772
DOI
10.1177/0023677217692372
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8508871
handle
http://hdl.handle.net/1854/LU-8508871
date created
2017-02-10 11:55:18
date last changed
2017-09-14 14:21:51
@article{8508871,
  abstract     = {Piglets are considered to be suitable animal models for predicting the pharmacokinetics and pharmacodynamics (PK/PD) of test drugs for potential use in the paediatric population. Such PK/PD studies require multiple blood and urine samplings. The goal of the present study was to determine a suitable blood collection strategy applicable in the youngest age categories of six days, four weeks and eight weeks of age, as well as a urine collection technique for male piglets in the same age categories. Blood was collected either by a surgically-placed jugular vein catheter (six days old [n\,=\,4] and four weeks old [n\,=\,2] piglets) or by direct venepuncture of the jugular vein (four weeks old [n\,=\,2] and eight weeks old [n\,=\,4] piglets). A non-invasive method for total volume urine collection in male piglets was also developed using a urine pouch. No specific complications were encountered during anaesthesia or surgery for jugular catheter placement. After a 24\,h recovery period, urine and blood were easily collected without technical complications. One piglet was humanely killed at week 2 because of septicaemia. Histological analysis of both veins in all four piglets revealed negligible damage to the blood vessel wall. In conclusion, the presented techniques for blood (jugular catheter and direct venepuncture) and urine collection (pouches) are suitable for PK/PD studies in piglets.},
  author       = {Gasthuys, Elke and Schauvliege, Stijn and van Bergen, Thomas and Millecam, Joske and Cerasoli, Ilaria and Martens, Ann and Gasthuys, Frank and Vandecasteele, Tim and Cornillie, Pieter and Van Den Broeck, Wim and Boyen, Filip and Croubels, Siska and Devreese, Mathias},
  issn         = {0023-6772},
  journal      = {LABORATORY ANIMALS},
  keyword      = {blood sampling,intravenous catheterization,neonate,piglet,urine collection},
  language     = {eng},
  title        = {Repetitive urine and blood sampling in neonatal and weaned piglets for pharmacokinetic and pharmacodynamic modelling in drug discovery : a pilot study},
  url          = {http://dx.doi.org/10.1177/0023677217692372},
  year         = {2017},
}

Chicago
Gasthuys, Elke, Stijn Schauvliege, Thomas van Bergen, Joske Millecam, Ilaria Cerasoli, Ann Martens, Frank Gasthuys, et al. 2017. “Repetitive Urine and Blood Sampling in Neonatal and Weaned Piglets for Pharmacokinetic and Pharmacodynamic Modelling in Drug Discovery : a Pilot Study.” Laboratory Animals.
APA
Gasthuys, E., Schauvliege, S., van Bergen, T., Millecam, J., Cerasoli, I., Martens, A., Gasthuys, F., et al. (2017). Repetitive urine and blood sampling in neonatal and weaned piglets for pharmacokinetic and pharmacodynamic modelling in drug discovery : a pilot study. LABORATORY ANIMALS.
Vancouver
1.
Gasthuys E, Schauvliege S, van Bergen T, Millecam J, Cerasoli I, Martens A, et al. Repetitive urine and blood sampling in neonatal and weaned piglets for pharmacokinetic and pharmacodynamic modelling in drug discovery : a pilot study. LABORATORY ANIMALS. 2017;
MLA
Gasthuys, Elke, Stijn Schauvliege, Thomas van Bergen, et al. “Repetitive Urine and Blood Sampling in Neonatal and Weaned Piglets for Pharmacokinetic and Pharmacodynamic Modelling in Drug Discovery : a Pilot Study.” LABORATORY ANIMALS (2017): n. pag. Print.