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Repetitive urine and blood sampling in neonatal and weaned piglets for pharmacokinetic and pharmacodynamic modelling in drug discovery : a pilot study

Elke Gasthuys (UGent) , Stijn Schauvliege (UGent) , Thomas van Bergen (UGent) , Joske Millecam (UGent) , Ilaria Cerasoli, Ann Martens (UGent) , Frank Gasthuys (UGent) , Tim Vandecasteele (UGent) , Pieter Cornillie (UGent) , Wim Van Den Broeck (UGent) , et al.
(2017) LABORATORY ANIMALS. 51(5). p.498-508
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Abstract
Piglets are considered to be suitable animal models for predicting the pharmacokinetics and pharmacodynamics (PK/PD) of test drugs for potential use in the paediatric population. Such PK/PD studies require multiple blood and urine samplings. The goal of the present study was to determine a suitable blood collection strategy applicable in the youngest age categories of six days, four weeks and eight weeks of age, as well as a urine collection technique for male piglets in the same age categories. Blood was collected either by a surgically-placed jugular vein catheter (six days old [n = 4] and four weeks old [n = 2] piglets) or by direct venepuncture of the jugular vein (four weeks old [n = 2] and eight weeks old [n = 4] piglets). A non-invasive method for total volume urine collection in male piglets was also developed using a urine pouch. No specific complications were encountered during anaesthesia or surgery for jugular catheter placement. After a 24 h recovery period, urine and blood were easily collected without technical complications. One piglet was humanely killed at week 2 because of septicaemia. Histological analysis of both veins in all four piglets revealed negligible damage to the blood vessel wall. In conclusion, the presented techniques for blood (jugular catheter and direct venepuncture) and urine collection (pouches) are suitable for PK/PD studies in piglets.
Keywords
blood sampling, intravenous catheterization, neonate, piglet, urine collection, PARENTERAL-NUTRITION, NEWBORN PIGLETS, CATHETERIZATION, PIGS, COLLECTION, PLACEMENT, SWINE

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Chicago
Gasthuys, Elke, Stijn Schauvliege, Thomas van Bergen, Joske Millecam, Ilaria Cerasoli, Ann Martens, Frank Gasthuys, et al. 2017. “Repetitive Urine and Blood Sampling in Neonatal and Weaned Piglets for Pharmacokinetic and Pharmacodynamic Modelling in Drug Discovery : a Pilot Study.” Laboratory Animals 51 (5): 498–508.
APA
Gasthuys, E., Schauvliege, S., van Bergen, T., Millecam, J., Cerasoli, I., Martens, A., Gasthuys, F., et al. (2017). Repetitive urine and blood sampling in neonatal and weaned piglets for pharmacokinetic and pharmacodynamic modelling in drug discovery : a pilot study. LABORATORY ANIMALS, 51(5), 498–508.
Vancouver
1.
Gasthuys E, Schauvliege S, van Bergen T, Millecam J, Cerasoli I, Martens A, et al. Repetitive urine and blood sampling in neonatal and weaned piglets for pharmacokinetic and pharmacodynamic modelling in drug discovery : a pilot study. LABORATORY ANIMALS. 2017;51(5):498–508.
MLA
Gasthuys, Elke, Stijn Schauvliege, Thomas van Bergen, et al. “Repetitive Urine and Blood Sampling in Neonatal and Weaned Piglets for Pharmacokinetic and Pharmacodynamic Modelling in Drug Discovery : a Pilot Study.” LABORATORY ANIMALS 51.5 (2017): 498–508. Print.
@article{8508871,
  abstract     = {Piglets are considered to be suitable animal models for predicting the pharmacokinetics and pharmacodynamics (PK/PD) of test drugs for potential use in the paediatric population. Such PK/PD studies require multiple blood and urine samplings. The goal of the present study was to determine a suitable blood collection strategy applicable in the youngest age categories of six days, four weeks and eight weeks of age, as well as a urine collection technique for male piglets in the same age categories. Blood was collected either by a surgically-placed jugular vein catheter (six days old [n\,=\,4] and four weeks old [n\,=\,2] piglets) or by direct venepuncture of the jugular vein (four weeks old [n\,=\,2] and eight weeks old [n\,=\,4] piglets). A non-invasive method for total volume urine collection in male piglets was also developed using a urine pouch. No specific complications were encountered during anaesthesia or surgery for jugular catheter placement. After a 24\,h recovery period, urine and blood were easily collected without technical complications. One piglet was humanely killed at week 2 because of septicaemia. Histological analysis of both veins in all four piglets revealed negligible damage to the blood vessel wall. In conclusion, the presented techniques for blood (jugular catheter and direct venepuncture) and urine collection (pouches) are suitable for PK/PD studies in piglets.},
  author       = {Gasthuys, Elke and Schauvliege, Stijn and van Bergen, Thomas and Millecam, Joske and Cerasoli, Ilaria and Martens, Ann and Gasthuys, Frank and Vandecasteele, Tim and Cornillie, Pieter and Van Den Broeck, Wim and Boyen, Filip and Croubels, Siska and Devreese, Mathias},
  issn         = {0023-6772},
  journal      = {LABORATORY ANIMALS},
  language     = {eng},
  number       = {5},
  pages        = {498--508},
  title        = {Repetitive urine and blood sampling in neonatal and weaned piglets for pharmacokinetic and pharmacodynamic modelling in drug discovery : a pilot study},
  url          = {http://dx.doi.org/10.1177/0023677217692372},
  volume       = {51},
  year         = {2017},
}

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