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Emerging role of extracellular vesicles in communication of preimplantation embryos in vitro

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Abstract
In vitro, efficient communication between mammalian embryos in groups or between embryos and cocultured somatic cells implies that there is a sender, a message and a receiver that is able to decode the message. Embryos secrete a variety of autocrine and paracrine factors and, of these, extracellular vesicles have recently been implicated as putative messengers in embryo-embryo communication, as well as in communication of the embryo with the maternal tract. Extracellular vesicles (EVs) are membrane-bound vesicles that are found in biofluids and in culture media conditioned by the presence of embryos or cells. EVs carry and transfer regulatory molecules, such as microRNAs, mRNAs, lipids and proteins. We conducted a systematic search of the literature to review and present the currently available evidence regarding the possible roles of EVs in in vitro embryo communication and embryo development. It is important to note that there is limited information available on the molecular mechanisms and many of the biologically plausible functions of EVs in embryo communication have not yet been substantiated by conclusive experimental evidence. However, indirect evidence, such as the use of media conditioned by embryos or by somatic cells with improved embryo development as a result, may indicate that EVs can be an important asset for the development of tailor-made media, allowing better embryo development in vitro, even for single embryo culture.
Keywords
embryo culture, embryo-maternal communication, OVIDUCT EPITHELIAL-CELLS, TUMOR-DERIVED MICROVESICLES, FEMALE REPRODUCTIVE-TRACT, BOVINE EMBRYOS, CONDITIONED MEDIUM, CULTURE-CONDITIONS, BLASTOCYST STAGE, APOPTOTIC BODIES, PLASMA-MEMBRANE, GENE-EXPRESSION

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Citation

Please use this url to cite or link to this publication:

Chicago
Pavani, Krishna, Carmen Alminana, Eline Wydooghe, Maaike Catteeuw, Miguel A Ramírez, Pascal Mermillod, Dimitrios Rizos, and Ann Van Soom. 2017. “Emerging Role of Extracellular Vesicles in Communication of Preimplantation Embryos in Vitro.” Reproduction Fertility and Development 29 (1): 66–83.
APA
Pavani, K., Alminana, C., Wydooghe, E., Catteeuw, M., Ramírez, M. A., Mermillod, P., Rizos, D., et al. (2017). Emerging role of extracellular vesicles in communication of preimplantation embryos in vitro. REPRODUCTION FERTILITY AND DEVELOPMENT, 29(1), 66–83. Presented at the Annual conference of the International Embryo Technology Society (IETS).
Vancouver
1.
Pavani K, Alminana C, Wydooghe E, Catteeuw M, Ramírez MA, Mermillod P, et al. Emerging role of extracellular vesicles in communication of preimplantation embryos in vitro. REPRODUCTION FERTILITY AND DEVELOPMENT. 2017;29(1):66–83.
MLA
Pavani, Krishna, Carmen Alminana, Eline Wydooghe, et al. “Emerging Role of Extracellular Vesicles in Communication of Preimplantation Embryos in Vitro.” REPRODUCTION FERTILITY AND DEVELOPMENT 29.1 (2017): 66–83. Print.
@article{8508754,
  abstract     = {In vitro, efficient communication between mammalian embryos in groups or between embryos and cocultured somatic cells implies that there is a sender, a message and a receiver that is able to decode the message. Embryos secrete a variety of autocrine and paracrine factors and, of these, extracellular vesicles have recently been implicated as putative messengers in embryo-embryo communication, as well as in communication of the embryo with the maternal tract. Extracellular vesicles (EVs) are membrane-bound vesicles that are found in biofluids and in culture media conditioned by the presence of embryos or cells. EVs carry and transfer regulatory molecules, such as microRNAs, mRNAs, lipids and proteins. We conducted a systematic search of the literature to review and present the currently available evidence regarding the possible roles of EVs in in vitro embryo communication and embryo development. It is important to note that there is limited information available on the molecular mechanisms and many of the biologically plausible functions of EVs in embryo communication have not yet been substantiated by conclusive experimental evidence. However, indirect evidence, such as the use of media conditioned by embryos or by somatic cells with improved embryo development as a result, may indicate that EVs can be an important asset for the development of tailor-made media, allowing better embryo development in vitro, even for single embryo culture.},
  author       = {Pavani, Krishna and Alminana, Carmen and Wydooghe, Eline and Catteeuw, Maaike and Ram{\'i}rez, Miguel A and Mermillod, Pascal and Rizos, Dimitrios and Van Soom, Ann},
  issn         = {1031-3613},
  journal      = {REPRODUCTION FERTILITY AND DEVELOPMENT},
  language     = {eng},
  location     = {Austin, TX, USA},
  number       = {1},
  pages        = {66--83},
  title        = {Emerging role of extracellular vesicles in communication of preimplantation embryos in vitro},
  url          = {http://dx.doi.org/10.1071/rd16318},
  volume       = {29},
  year         = {2017},
}

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