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Surface colonization and activity of the 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. strain MSH1 at macro- and micropollutant BAM concentrations

(2016) ENVIRONMENTAL SCIENCE & TECHNOLOGY. 50(18). p.10123-10133
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Abstract
Aminobacter sp. MSH1 uses the groundwater micropollutant 2,6-dichlorobenzamide (BAM) as a C and N source and is a potential catalyst for biotreatment of BAM-contaminated groundwater in filtration units of drinking water treatment plants (DWTPs). The oligotrophic environment of DWTPs including trace pollutant concentrations, and the high flow rates impose challenges for micropollutant biodegradation in DWTPs. To understand how trace BAM concentrations affect MSH1 surface colonization and BAM degrading activity, MSH1 was cultivated in flow channels fed continuously with BAM macro- and microconcentrations in a N- and C-limiting medium. At all BAM concentrations, MSH1 colonized the flow channel. BAM degradation efficiencies were concentration-dependent, ranging between 70 and 95%. Similarly, BAM concentration affected surface colonization, but at 100 mu g/L BAM and lower, colonization was similar to that in systems without BAM, suggesting that assimilable organic carbon and nitrogen other than those supplied by BAM sustained colonization at BAM microconcentrations. Comparison of specific BAM degradation rates in flow channels and in cultures of suspended freshly grown cells indicated that starvation conditions in flow channels receiving BAM microconcentrations resulted into MSH1 biomasses with 10-100-times reduced BAM degrading activity and provided a kinetic model for predicting BAM degradation under continuous C and N starvation.
Keywords
PERSISTENT METABOLITE 2, 6-DICHLOROBENZAMIDE, ASSIMILABLE ORGANIC-CARBON, HERBICIDE DICHLOBENIL, DRINKING-WATER, PSEUDOMONAS-AERUGINOSA, BIOFILM, DEGRADATION, MINERALIZATION, BIODEGRADATION, SOILS

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Chicago
Sekhar, Aswini, Benjamin Horemans, Jens Aamand, Sebastian R Sørensen, Lynn Vanhaecke, Julie Vanden Bussche, Johan Hofkens, and Dirk Springael. 2016. “Surface Colonization and Activity of the 2,6-dichlorobenzamide (BAM) Degrading Aminobacter Sp. Strain MSH1 at Macro- and Micropollutant BAM Concentrations.” Environmental Science & Technology 50 (18): 10123–10133.
APA
Sekhar, A., Horemans, B., Aamand, J., Sørensen, S. R., Vanhaecke, L., Vanden Bussche, J., Hofkens, J., et al. (2016). Surface colonization and activity of the 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. strain MSH1 at macro- and micropollutant BAM concentrations. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 50(18), 10123–10133.
Vancouver
1.
Sekhar A, Horemans B, Aamand J, Sørensen SR, Vanhaecke L, Vanden Bussche J, et al. Surface colonization and activity of the 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. strain MSH1 at macro- and micropollutant BAM concentrations. ENVIRONMENTAL SCIENCE & TECHNOLOGY. 2016;50(18):10123–33.
MLA
Sekhar, Aswini, Benjamin Horemans, Jens Aamand, et al. “Surface Colonization and Activity of the 2,6-dichlorobenzamide (BAM) Degrading Aminobacter Sp. Strain MSH1 at Macro- and Micropollutant BAM Concentrations.” ENVIRONMENTAL SCIENCE & TECHNOLOGY 50.18 (2016): 10123–10133. Print.
@article{8508061,
  abstract     = {Aminobacter sp. MSH1 uses the groundwater micropollutant 2,6-dichlorobenzamide (BAM) as a C and N source and is a potential catalyst for biotreatment of BAM-contaminated groundwater in filtration units of drinking water treatment plants (DWTPs). The oligotrophic environment of DWTPs including trace pollutant concentrations, and the high flow rates impose challenges for micropollutant biodegradation in DWTPs. To understand how trace BAM concentrations affect MSH1 surface colonization and BAM degrading activity, MSH1 was cultivated in flow channels fed continuously with BAM macro- and microconcentrations in a N- and C-limiting medium. At all BAM concentrations, MSH1 colonized the flow channel. BAM degradation efficiencies were concentration-dependent, ranging between 70 and 95\%. Similarly, BAM concentration affected surface colonization, but at 100 mu g/L BAM and lower, colonization was similar to that in systems without BAM, suggesting that assimilable organic carbon and nitrogen other than those supplied by BAM sustained colonization at BAM microconcentrations. Comparison of specific BAM degradation rates in flow channels and in cultures of suspended freshly grown cells indicated that starvation conditions in flow channels receiving BAM microconcentrations resulted into MSH1 biomasses with 10-100-times reduced BAM degrading activity and provided a kinetic model for predicting BAM degradation under continuous C and N starvation.},
  author       = {Sekhar, Aswini and Horemans, Benjamin and Aamand, Jens and S{\o}rensen, Sebastian R and Vanhaecke, Lynn and Vanden Bussche, Julie and Hofkens, Johan and Springael, Dirk},
  issn         = {0013-936X},
  journal      = {ENVIRONMENTAL SCIENCE \& TECHNOLOGY},
  keyword      = {PERSISTENT METABOLITE 2,6-DICHLOROBENZAMIDE,ASSIMILABLE ORGANIC-CARBON,HERBICIDE DICHLOBENIL,DRINKING-WATER,PSEUDOMONAS-AERUGINOSA,BIOFILM,DEGRADATION,MINERALIZATION,BIODEGRADATION,SOILS},
  language     = {eng},
  number       = {18},
  pages        = {10123--10133},
  title        = {Surface colonization and activity of the 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. strain MSH1 at macro- and micropollutant BAM concentrations},
  url          = {http://dx.doi.org/10.1021/acs.est.6b01978},
  volume       = {50},
  year         = {2016},
}

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